Effects of dietary administration of fenugreek seeds, alone or in combination with probiotics, on growth performance parameters, humoral immune response and gene expression of gilthead seabream (Sparus aurata L.)

Andrea Santulli, Concetta Maria Messina, Amina Bakhrouf, Cerezuela, María Ángeles Esteban, Abdelkarim Mahdhi, Guardiola, Bahi, Francisco A. Guardiola

Risultato della ricerca: Articlepeer review

40 Citazioni (Scopus)

Abstract

The use of immunostimulants is considered a promising preventive practice that may help to maintainanimal welfare and a healthy environment, while increasing production and providing higher profits. Thepurpose of this study was to evaluate the effects on gilthead seabream (Sparus aurata L.) of the dietaryadministration of fenugreek (Trigonella foenum graecum) seeds, alone or combined with one of thefollowing probiotic strains: Bacillus licheniformis (TSB27), Lactobacillus plantarum or Bacillus subtilis (B46).Gilthead seabream were fed a control or one of the supplemented diets for 3 weeks. The effects of thesesupplemented diets on growth performance parameters and the humoral immune response (naturalhaemolytic complement, peroxidase, total IgM levels, proteases and antiproteases activities) wereevaluated after 2 and 3 weeks of feeding. Simultaneously, the expression levels of some immunerelevantgenes (igm, tcr-b, csfr1 and bd) were measured in the head-kidney. Interestingly, all probioticsupplemented diets increased seabream growth rates, especially the B. licheniformis supplemented diet.Generally, humoral immune parameters were enhanced by the dietary supplementation at the differenttime points measured. The results showed a significant increases in the immune parameters, principallyin fish fed only fenugreek or fenugreek combined with B. subtilis. Furthermore, real time qPCR revealedthat dietary supplementation significantly enhances the expression of immune-associated genes in thehead-kidney, particularly igm gene expression. These results suggest that fenugreek alone or combinedwith one of the probiotic strains mentioned enhances the immune response of gilthead seabream, aspecies with one of the highest rates of production in marine aquaculture.
Lingua originaleEnglish
pagine (da-a)50-58
Numero di pagine9
RivistaFISH AND SHELLFISH IMMUNOLOGY
Volume60
Stato di pubblicazionePublished - 2017

All Science Journal Classification (ASJC) codes

  • ???subjectarea.asjc.2300.2304???
  • ???subjectarea.asjc.1100.1104???

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