Effects of a GH polymorphism on milk production traits in Modicana and Cinisara cows reared in different feeding systems.

Adriana Bonanno, Antonino Di Grigoli, Dégi, Agnieszka Kolacinska, Selby, Danica Rotar Pavlic, Ian Banks, Sébastien Moine, Malas, Marc Beishon, Weller, Aapro, Richard Price, Marije Bolt, Claire Champeix, Bonanno, Lisa Jelf Eneqvist, Joanna Kazmierska, Richard Price, SelbyMehmet Ungan, Wyld, Walter, Joanna Kazmierska

Risultato della ricerca: Meeting Abstract

1 Citazione (Scopus)

Abstract

Growth hormone, a polypeptide encoded by the GH gene, is a member of the somatotropin/prolactin family of hormones, which plays an important role on milk production and metabolism. In bovine, a single nucleotide polymorphism (SNP) in exon 5 changes leucine to valine (CTG to GTG) in the mature GH molecule. The purpose of this study was to evaluate, in Modicana and Cinisara cows, the effects of genetic polymorphism at GH locus and its interaction with feeding system on milk traits. A total of 97 individual Modicana blood samples were collected, 65 from a semi extensive and 32 from an extensive farm. A total of 58 Cinisara individual milk samples were collected, 32 from 7 extensive farms and 26 from 6 semi-extensive farms. In the extensive farms the cows were fed exclusively with pasture; in the semi-extensive farms cows were fed with pasture, hay and concentrate. DNA was extracted from Modicana white blood cells and from Cinisara milk somatic cells. Bovine Growth Hormone (bGH) was analyzed at the 5th exon according to Komisarek et al (2011). In Modicana farms, monthly, individual milk yield was recorded and individual milk samples collected. In Cinisara farms milk production was recorded once, the day of samples collection. Fat and protein were determined (Combi-foss 6000, Foss Electric, Hillerød, Denmark). In Modicana, milk yield, fat and protein were analysed using the GLM procedure for repeated measures of SPSS (SPSS for Windows, SPSS Inc., Chicago IL, USA). In Cinisara, milk yield, fat and protein were analysed using the univariate GLM procedure of SPSS. The analyses included main effects of GH genotype, feeding system and the interaction genotype × feeding system. Three genotypes were found:: LL (236, 132, 36bp), LV (236, 185, 132, 51, 36 bp) and VV (185, 132, 51, 36 bp). Genotype frequencies, respectively in Modicana and Cinisara breeds were: LL, 0.61 and 0.34; LV, 0.37 and 0.57; VV, 0.02 and 0.09. Milk production and gross composition were not influenzed by GH genotype (respectively in Modicana and Cinisara: milk yield, LL 9.06 and 12.3, LV 9.24 and 11.4, VV 7.61 and 8.33; fat, LL 4.08 and 3.32, LV 3.91 and 3.13, VV 4.04 and 3.62; protein, LL 3.67 and 3.51, LV 3.66 and 3.56, VV 3.64 and 3.71). These results are in line with different authors. The higher energy level of the diets offered in the semi extensive farms did not interfere with GH genotype effect on milk traits: no significant interaction was found between genotype and feeding system.
Lingua originaleEnglish
pagine (da-a)187-188
Numero di pagine2
RivistaITALIAN JOURNAL OF ANIMAL SCIENCE
Volumevolume 18
Stato di pubblicazionePublished - 2019

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Milk
Genotype
Fats
Growth Hormone
Exons
Proteins
Farms
Valine
Genetic Polymorphisms
Denmark
Leucine
Prolactin
Single Nucleotide Polymorphism
Leukocytes
Hormones
Diet
Peptides
DNA

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology

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Effects of a GH polymorphism on milk production traits in Modicana and Cinisara cows reared in different feeding systems. / Bonanno, Adriana; Di Grigoli, Antonino; Dégi; Kolacinska, Agnieszka; Selby; Pavlic, Danica Rotar; Banks, Ian; Moine, Sébastien; Malas; Beishon, Marc; Weller; Aapro; Price, Richard; Bolt, Marije; Champeix, Claire; Bonanno; Eneqvist, Lisa Jelf; Kazmierska, Joanna; Price, Richard; Selby; Ungan, Mehmet; Wyld; Walter; Kazmierska, Joanna.

In: ITALIAN JOURNAL OF ANIMAL SCIENCE, Vol. volume 18, 2019, pag. 187-188.

Risultato della ricerca: Meeting Abstract

Bonanno, A, Di Grigoli, A, Dégi, Kolacinska, A, Selby, Pavlic, DR, Banks, I, Moine, S, Malas, Beishon, M, Weller, Aapro, Price, R, Bolt, M, Champeix, C, Bonanno, Eneqvist, LJ, Kazmierska, J, Price, R, Selby, Ungan, M, Wyld, Walter & Kazmierska, J 2019, 'Effects of a GH polymorphism on milk production traits in Modicana and Cinisara cows reared in different feeding systems.', ITALIAN JOURNAL OF ANIMAL SCIENCE, vol. volume 18, pagg. 187-188.
Bonanno, Adriana ; Di Grigoli, Antonino ; Dégi ; Kolacinska, Agnieszka ; Selby ; Pavlic, Danica Rotar ; Banks, Ian ; Moine, Sébastien ; Malas ; Beishon, Marc ; Weller ; Aapro ; Price, Richard ; Bolt, Marije ; Champeix, Claire ; Bonanno ; Eneqvist, Lisa Jelf ; Kazmierska, Joanna ; Price, Richard ; Selby ; Ungan, Mehmet ; Wyld ; Walter ; Kazmierska, Joanna. / Effects of a GH polymorphism on milk production traits in Modicana and Cinisara cows reared in different feeding systems. In: ITALIAN JOURNAL OF ANIMAL SCIENCE. 2019 ; Vol. volume 18. pagg. 187-188.
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title = "Effects of a GH polymorphism on milk production traits in Modicana and Cinisara cows reared in different feeding systems.",
abstract = "Growth hormone, a polypeptide encoded by the GH gene, is a member of the somatotropin/prolactin family of hormones, which plays an important role on milk production and metabolism. In bovine, a single nucleotide polymorphism (SNP) in exon 5 changes leucine to valine (CTG to GTG) in the mature GH molecule. The purpose of this study was to evaluate, in Modicana and Cinisara cows, the effects of genetic polymorphism at GH locus and its interaction with feeding system on milk traits. A total of 97 individual Modicana blood samples were collected, 65 from a semi extensive and 32 from an extensive farm. A total of 58 Cinisara individual milk samples were collected, 32 from 7 extensive farms and 26 from 6 semi-extensive farms. In the extensive farms the cows were fed exclusively with pasture; in the semi-extensive farms cows were fed with pasture, hay and concentrate. DNA was extracted from Modicana white blood cells and from Cinisara milk somatic cells. Bovine Growth Hormone (bGH) was analyzed at the 5th exon according to Komisarek et al (2011). In Modicana farms, monthly, individual milk yield was recorded and individual milk samples collected. In Cinisara farms milk production was recorded once, the day of samples collection. Fat and protein were determined (Combi-foss 6000, Foss Electric, Hiller{\o}d, Denmark). In Modicana, milk yield, fat and protein were analysed using the GLM procedure for repeated measures of SPSS (SPSS for Windows, SPSS Inc., Chicago IL, USA). In Cinisara, milk yield, fat and protein were analysed using the univariate GLM procedure of SPSS. The analyses included main effects of GH genotype, feeding system and the interaction genotype × feeding system. Three genotypes were found:: LL (236, 132, 36bp), LV (236, 185, 132, 51, 36 bp) and VV (185, 132, 51, 36 bp). Genotype frequencies, respectively in Modicana and Cinisara breeds were: LL, 0.61 and 0.34; LV, 0.37 and 0.57; VV, 0.02 and 0.09. Milk production and gross composition were not influenzed by GH genotype (respectively in Modicana and Cinisara: milk yield, LL 9.06 and 12.3, LV 9.24 and 11.4, VV 7.61 and 8.33; fat, LL 4.08 and 3.32, LV 3.91 and 3.13, VV 4.04 and 3.62; protein, LL 3.67 and 3.51, LV 3.66 and 3.56, VV 3.64 and 3.71). These results are in line with different authors. The higher energy level of the diets offered in the semi extensive farms did not interfere with GH genotype effect on milk traits: no significant interaction was found between genotype and feeding system.",
author = "Adriana Bonanno and {Di Grigoli}, Antonino and D{\'e}gi and Agnieszka Kolacinska and Selby and Pavlic, {Danica Rotar} and Ian Banks and S{\'e}bastien Moine and Malas and Marc Beishon and Weller and Aapro and Richard Price and Marije Bolt and Claire Champeix and Bonanno and Eneqvist, {Lisa Jelf} and Joanna Kazmierska and Richard Price and Selby and Mehmet Ungan and Wyld and Walter and Joanna Kazmierska",
year = "2019",
language = "English",
volume = "volume 18",
pages = "187--188",
journal = "ITALIAN JOURNAL OF ANIMAL SCIENCE",
issn = "1828-051X",

}

TY - JOUR

T1 - Effects of a GH polymorphism on milk production traits in Modicana and Cinisara cows reared in different feeding systems.

AU - Bonanno, Adriana

AU - Di Grigoli, Antonino

AU - Dégi, null

AU - Kolacinska, Agnieszka

AU - Selby, null

AU - Pavlic, Danica Rotar

AU - Banks, Ian

AU - Moine, Sébastien

AU - Malas, null

AU - Beishon, Marc

AU - Weller, null

AU - Aapro, null

AU - Price, Richard

AU - Bolt, Marije

AU - Champeix, Claire

AU - Bonanno, null

AU - Eneqvist, Lisa Jelf

AU - Kazmierska, Joanna

AU - Price, Richard

AU - Selby, null

AU - Ungan, Mehmet

AU - Wyld, null

AU - Walter, null

AU - Kazmierska, Joanna

PY - 2019

Y1 - 2019

N2 - Growth hormone, a polypeptide encoded by the GH gene, is a member of the somatotropin/prolactin family of hormones, which plays an important role on milk production and metabolism. In bovine, a single nucleotide polymorphism (SNP) in exon 5 changes leucine to valine (CTG to GTG) in the mature GH molecule. The purpose of this study was to evaluate, in Modicana and Cinisara cows, the effects of genetic polymorphism at GH locus and its interaction with feeding system on milk traits. A total of 97 individual Modicana blood samples were collected, 65 from a semi extensive and 32 from an extensive farm. A total of 58 Cinisara individual milk samples were collected, 32 from 7 extensive farms and 26 from 6 semi-extensive farms. In the extensive farms the cows were fed exclusively with pasture; in the semi-extensive farms cows were fed with pasture, hay and concentrate. DNA was extracted from Modicana white blood cells and from Cinisara milk somatic cells. Bovine Growth Hormone (bGH) was analyzed at the 5th exon according to Komisarek et al (2011). In Modicana farms, monthly, individual milk yield was recorded and individual milk samples collected. In Cinisara farms milk production was recorded once, the day of samples collection. Fat and protein were determined (Combi-foss 6000, Foss Electric, Hillerød, Denmark). In Modicana, milk yield, fat and protein were analysed using the GLM procedure for repeated measures of SPSS (SPSS for Windows, SPSS Inc., Chicago IL, USA). In Cinisara, milk yield, fat and protein were analysed using the univariate GLM procedure of SPSS. The analyses included main effects of GH genotype, feeding system and the interaction genotype × feeding system. Three genotypes were found:: LL (236, 132, 36bp), LV (236, 185, 132, 51, 36 bp) and VV (185, 132, 51, 36 bp). Genotype frequencies, respectively in Modicana and Cinisara breeds were: LL, 0.61 and 0.34; LV, 0.37 and 0.57; VV, 0.02 and 0.09. Milk production and gross composition were not influenzed by GH genotype (respectively in Modicana and Cinisara: milk yield, LL 9.06 and 12.3, LV 9.24 and 11.4, VV 7.61 and 8.33; fat, LL 4.08 and 3.32, LV 3.91 and 3.13, VV 4.04 and 3.62; protein, LL 3.67 and 3.51, LV 3.66 and 3.56, VV 3.64 and 3.71). These results are in line with different authors. The higher energy level of the diets offered in the semi extensive farms did not interfere with GH genotype effect on milk traits: no significant interaction was found between genotype and feeding system.

AB - Growth hormone, a polypeptide encoded by the GH gene, is a member of the somatotropin/prolactin family of hormones, which plays an important role on milk production and metabolism. In bovine, a single nucleotide polymorphism (SNP) in exon 5 changes leucine to valine (CTG to GTG) in the mature GH molecule. The purpose of this study was to evaluate, in Modicana and Cinisara cows, the effects of genetic polymorphism at GH locus and its interaction with feeding system on milk traits. A total of 97 individual Modicana blood samples were collected, 65 from a semi extensive and 32 from an extensive farm. A total of 58 Cinisara individual milk samples were collected, 32 from 7 extensive farms and 26 from 6 semi-extensive farms. In the extensive farms the cows were fed exclusively with pasture; in the semi-extensive farms cows were fed with pasture, hay and concentrate. DNA was extracted from Modicana white blood cells and from Cinisara milk somatic cells. Bovine Growth Hormone (bGH) was analyzed at the 5th exon according to Komisarek et al (2011). In Modicana farms, monthly, individual milk yield was recorded and individual milk samples collected. In Cinisara farms milk production was recorded once, the day of samples collection. Fat and protein were determined (Combi-foss 6000, Foss Electric, Hillerød, Denmark). In Modicana, milk yield, fat and protein were analysed using the GLM procedure for repeated measures of SPSS (SPSS for Windows, SPSS Inc., Chicago IL, USA). In Cinisara, milk yield, fat and protein were analysed using the univariate GLM procedure of SPSS. The analyses included main effects of GH genotype, feeding system and the interaction genotype × feeding system. Three genotypes were found:: LL (236, 132, 36bp), LV (236, 185, 132, 51, 36 bp) and VV (185, 132, 51, 36 bp). Genotype frequencies, respectively in Modicana and Cinisara breeds were: LL, 0.61 and 0.34; LV, 0.37 and 0.57; VV, 0.02 and 0.09. Milk production and gross composition were not influenzed by GH genotype (respectively in Modicana and Cinisara: milk yield, LL 9.06 and 12.3, LV 9.24 and 11.4, VV 7.61 and 8.33; fat, LL 4.08 and 3.32, LV 3.91 and 3.13, VV 4.04 and 3.62; protein, LL 3.67 and 3.51, LV 3.66 and 3.56, VV 3.64 and 3.71). These results are in line with different authors. The higher energy level of the diets offered in the semi extensive farms did not interfere with GH genotype effect on milk traits: no significant interaction was found between genotype and feeding system.

UR - http://hdl.handle.net/10447/362272

M3 - Meeting Abstract

VL - volume 18

SP - 187

EP - 188

JO - ITALIAN JOURNAL OF ANIMAL SCIENCE

JF - ITALIAN JOURNAL OF ANIMAL SCIENCE

SN - 1828-051X

ER -