Ectopic expression of CXCL13, BAFF, APRIL and LT-ß is associated with artery tertiary lymphoid organs in giant cell arteritis

Giuliana Guggino, Francesco Ciccia, Riccardo Alessandro, Giovanni Triolo, Stefania Raimondo, Aroldo Rizzo, Alessandra Cannizzaro, Stefania Croci, Alberto Cavazza, Carlo Salvarani, Alessandra Cannizzaro

Risultato della ricerca: Article

21 Citazioni (Scopus)

Abstract

Objectives To investigate whether artery tertiary lymphoid organs (ATLOs) are present in giant cell arteritis (GCA) and that their formation is associated with the ectopic expression of constitutive lymphoid tissue-homing chemokines. Methods Reverse transcriptase PCR, immunohistochemical and immunofluorescence analysis were used to determine the presence of ectopic ATLOs in GCA and the expression of chemokines/chemokine receptors and cytokines involved in lymphoneogenesis in the temporal artery samples obtained from 50 patients with GCA and 30 controls. The presence of lymphatic conduits, of follicular dendritic cells (FDCs) precursors and lymphoid tissue inducer cells was also investigated. Finally, expression of CXCL13, B cell activating factor (BAFF), a proliferation-inducing ligand (APRIL) and CCL21 by isolated myofibroblasts was evaluated before and after stimulation with Toll-like receptors (TLRs) agonists and cytokines. Results ATLOs were observed in the media layer of 60% of patients with GCA in close proximity to high endothelial venules and independently by the age of patients and the presence of atherosclerosis. ATLO formation was also accompanied by the expression of CXCL13, BAFF, a proliferation-inducing ligand (APRIL), lymphotoxin (LT)-ß, interleukin (IL)-17 and IL-7, the presence of FDC precursors and of lymphoid conduits. Stimulation of myofibroblasts with TLR agonists and cytokines resulted in the upregulation of BAFF and CXCL13. Conclusions ATLOs occur in the inflamed arteries of patients with GCA possibly representing the immune sites where immune responses towards unknown arterial wall-derived antigens may be organised.
Lingua originaleEnglish
pagine (da-a)annrheumdis-2016-209217-
Numero di pagine9
RivistaDefault journal
Stato di pubblicazionePublished - 2016

Fingerprint

Lymphotoxin-alpha
Giant Cell Arteritis
Arteries
Toll-Like Receptors
Cytokines
Ligands
Chemokines
B-Cell Activating Factor
Follicular Dendritic Cells
Tissue
Interleukin-7
Myofibroblasts
Interleukin-17
Chemokine Receptors
RNA-Directed DNA Polymerase
Lymphoid Tissue
Antigens
Temporal Arteries
Venules
Helper-Inducer T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology and Allergy
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

Cita questo

Ectopic expression of CXCL13, BAFF, APRIL and LT-ß is associated with artery tertiary lymphoid organs in giant cell arteritis. / Guggino, Giuliana; Ciccia, Francesco; Alessandro, Riccardo; Triolo, Giovanni; Raimondo, Stefania; Rizzo, Aroldo; Cannizzaro, Alessandra; Croci, Stefania; Cavazza, Alberto; Salvarani, Carlo; Cannizzaro, Alessandra.

In: Default journal, 2016, pag. annrheumdis-2016-209217-.

Risultato della ricerca: Article

Guggino, Giuliana ; Ciccia, Francesco ; Alessandro, Riccardo ; Triolo, Giovanni ; Raimondo, Stefania ; Rizzo, Aroldo ; Cannizzaro, Alessandra ; Croci, Stefania ; Cavazza, Alberto ; Salvarani, Carlo ; Cannizzaro, Alessandra. / Ectopic expression of CXCL13, BAFF, APRIL and LT-ß is associated with artery tertiary lymphoid organs in giant cell arteritis. In: Default journal. 2016 ; pagg. annrheumdis-2016-209217-.
@article{a2a5c77c5f864fb9a35824756ef01d6e,
title = "Ectopic expression of CXCL13, BAFF, APRIL and LT-{\ss} is associated with artery tertiary lymphoid organs in giant cell arteritis",
abstract = "Objectives To investigate whether artery tertiary lymphoid organs (ATLOs) are present in giant cell arteritis (GCA) and that their formation is associated with the ectopic expression of constitutive lymphoid tissue-homing chemokines. Methods Reverse transcriptase PCR, immunohistochemical and immunofluorescence analysis were used to determine the presence of ectopic ATLOs in GCA and the expression of chemokines/chemokine receptors and cytokines involved in lymphoneogenesis in the temporal artery samples obtained from 50 patients with GCA and 30 controls. The presence of lymphatic conduits, of follicular dendritic cells (FDCs) precursors and lymphoid tissue inducer cells was also investigated. Finally, expression of CXCL13, B cell activating factor (BAFF), a proliferation-inducing ligand (APRIL) and CCL21 by isolated myofibroblasts was evaluated before and after stimulation with Toll-like receptors (TLRs) agonists and cytokines. Results ATLOs were observed in the media layer of 60{\%} of patients with GCA in close proximity to high endothelial venules and independently by the age of patients and the presence of atherosclerosis. ATLO formation was also accompanied by the expression of CXCL13, BAFF, a proliferation-inducing ligand (APRIL), lymphotoxin (LT)-{\ss}, interleukin (IL)-17 and IL-7, the presence of FDC precursors and of lymphoid conduits. Stimulation of myofibroblasts with TLR agonists and cytokines resulted in the upregulation of BAFF and CXCL13. Conclusions ATLOs occur in the inflamed arteries of patients with GCA possibly representing the immune sites where immune responses towards unknown arterial wall-derived antigens may be organised.",
author = "Giuliana Guggino and Francesco Ciccia and Riccardo Alessandro and Giovanni Triolo and Stefania Raimondo and Aroldo Rizzo and Alessandra Cannizzaro and Stefania Croci and Alberto Cavazza and Carlo Salvarani and Alessandra Cannizzaro",
year = "2016",
language = "English",
pages = "annrheumdis--2016--209217--",
journal = "Default journal",

}

TY - JOUR

T1 - Ectopic expression of CXCL13, BAFF, APRIL and LT-ß is associated with artery tertiary lymphoid organs in giant cell arteritis

AU - Guggino, Giuliana

AU - Ciccia, Francesco

AU - Alessandro, Riccardo

AU - Triolo, Giovanni

AU - Raimondo, Stefania

AU - Rizzo, Aroldo

AU - Cannizzaro, Alessandra

AU - Croci, Stefania

AU - Cavazza, Alberto

AU - Salvarani, Carlo

AU - Cannizzaro, Alessandra

PY - 2016

Y1 - 2016

N2 - Objectives To investigate whether artery tertiary lymphoid organs (ATLOs) are present in giant cell arteritis (GCA) and that their formation is associated with the ectopic expression of constitutive lymphoid tissue-homing chemokines. Methods Reverse transcriptase PCR, immunohistochemical and immunofluorescence analysis were used to determine the presence of ectopic ATLOs in GCA and the expression of chemokines/chemokine receptors and cytokines involved in lymphoneogenesis in the temporal artery samples obtained from 50 patients with GCA and 30 controls. The presence of lymphatic conduits, of follicular dendritic cells (FDCs) precursors and lymphoid tissue inducer cells was also investigated. Finally, expression of CXCL13, B cell activating factor (BAFF), a proliferation-inducing ligand (APRIL) and CCL21 by isolated myofibroblasts was evaluated before and after stimulation with Toll-like receptors (TLRs) agonists and cytokines. Results ATLOs were observed in the media layer of 60% of patients with GCA in close proximity to high endothelial venules and independently by the age of patients and the presence of atherosclerosis. ATLO formation was also accompanied by the expression of CXCL13, BAFF, a proliferation-inducing ligand (APRIL), lymphotoxin (LT)-ß, interleukin (IL)-17 and IL-7, the presence of FDC precursors and of lymphoid conduits. Stimulation of myofibroblasts with TLR agonists and cytokines resulted in the upregulation of BAFF and CXCL13. Conclusions ATLOs occur in the inflamed arteries of patients with GCA possibly representing the immune sites where immune responses towards unknown arterial wall-derived antigens may be organised.

AB - Objectives To investigate whether artery tertiary lymphoid organs (ATLOs) are present in giant cell arteritis (GCA) and that their formation is associated with the ectopic expression of constitutive lymphoid tissue-homing chemokines. Methods Reverse transcriptase PCR, immunohistochemical and immunofluorescence analysis were used to determine the presence of ectopic ATLOs in GCA and the expression of chemokines/chemokine receptors and cytokines involved in lymphoneogenesis in the temporal artery samples obtained from 50 patients with GCA and 30 controls. The presence of lymphatic conduits, of follicular dendritic cells (FDCs) precursors and lymphoid tissue inducer cells was also investigated. Finally, expression of CXCL13, B cell activating factor (BAFF), a proliferation-inducing ligand (APRIL) and CCL21 by isolated myofibroblasts was evaluated before and after stimulation with Toll-like receptors (TLRs) agonists and cytokines. Results ATLOs were observed in the media layer of 60% of patients with GCA in close proximity to high endothelial venules and independently by the age of patients and the presence of atherosclerosis. ATLO formation was also accompanied by the expression of CXCL13, BAFF, a proliferation-inducing ligand (APRIL), lymphotoxin (LT)-ß, interleukin (IL)-17 and IL-7, the presence of FDC precursors and of lymphoid conduits. Stimulation of myofibroblasts with TLR agonists and cytokines resulted in the upregulation of BAFF and CXCL13. Conclusions ATLOs occur in the inflamed arteries of patients with GCA possibly representing the immune sites where immune responses towards unknown arterial wall-derived antigens may be organised.

UR - http://hdl.handle.net/10447/206673

UR - http://ard.bmj.com/

M3 - Article

SP - annrheumdis-2016-209217-

JO - Default journal

JF - Default journal

ER -