Early asymmetric cues triggering the Dorsal/Ventral Gene Regulatory Network of the sea urchin embryo

Risultato della ricerca: Articlepeer review

20 Citazioni (Scopus)

Abstract

Dorsal/ventral (DV) patterning of the sea urchin embryo relies on a ventrally-localized organizer expressing Nodal, a pivotal regulator of the DV gene regulatory network. However, the inceptive mechanisms imposing the symmetry-breaking are incompletely understood. In Paracentrotus lividus, the Hbox12 homeodomain-containing repressor is expressed by prospective dorsal cells, spatially facing and preceding the onset of nodal transcription. We report that Hbox12 misexpression provokes DV abnormalities, attenuating nodal and nodal-dependent transcription. Reciprocally, impairing hbox12 function disrupts DV polarity by allowing ectopic expression of nodal. Clonal loss-of-function, inflicted by blastomere transplantation or gene-transfer assays, highlights that DV polarization requires Hbox12 action in dorsal cells. Remarkably, the localized knock-down of nodal restores DV polarity of embryos lacking hbox12 function. Finally, we show that hbox12 is a dorsal-specific negative modulator of the p38-MAPK activity, which is required for nodal expression. Altogether, our results suggest that Hbox12 function is essential for proper positioning of the DV organizer. -
Lingua originaleEnglish
Numero di pagine21
RivistaeLife
VolumeeLife 2014;3:e04664
Stato di pubblicazionePublished - 2014

All Science Journal Classification (ASJC) codes

  • ???subjectarea.asjc.2800.2800???
  • ???subjectarea.asjc.1300.1300???
  • ???subjectarea.asjc.2400.2400???

Fingerprint Entra nei temi di ricerca di 'Early asymmetric cues triggering the Dorsal/Ventral Gene Regulatory Network of the sea urchin embryo'. Insieme formano una fingerprint unica.

Cita questo