Duodenal contractile activity in dystrophic (mdx) mice: Reduction of nitric oxide influence

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Abstract

The present study was undertaken to analyse duodenal contractility in adult dystrophic (mdx) mice. The spontaneous changes of the isometric tension and the responses of longitudinal duodenal muscle to nonadrenergic, noncholinergic (NANC) nerve stimulation and to exogenous drugs were compared between normal and mdx mice. Duodenal segments from mdx mice displayed spontaneous contractions with higher frequency than normals. Nω-nitro-L-arginine methyl ester (L-NAME) increased the frequency of contractions in normals without affecting that in mdx mice. In normals, NANC nerve stimulation elicited a transient relaxation abolished by L-NAME. In mdx mice a frank relaxation was not observed, the inhibitory response consisted just in the suppression of the phasic activity. This response was reduced by L-NAME and abolished by the subsequent addition of α-chymotrypsin. In normals, α-chymo-trypsin hardly affected NANC relaxation, whilst it significantly antagonised that in mdx mice. Mdx duodenal muscle also showed a reduced responsiveness to sodium nitroprusside, and to 8-bromoguanosine 3′, 5′-cyclic monophosphate in comparison with normal preparations. The results indicate that mdx mice experience duodenal contractile disturbances due to an impairment of NO function with defective responsiveness of the muscle to NO. The reduction in NO influence is functionally compensated by the peptidergic system.
Lingua originaleEnglish
pagine (da-a)559-565
Numero di pagine7
RivistaNeurogastroenterology and Motility
Volume15
Stato di pubblicazionePublished - 2003

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Inbred mdx Mouse
Nitric Oxide
NG-Nitroarginine Methyl Ester
Muscles
Chymotrypsin
Nitroprusside
Trypsin

All Science Journal Classification (ASJC) codes

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

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title = "Duodenal contractile activity in dystrophic (mdx) mice: Reduction of nitric oxide influence",
abstract = "The present study was undertaken to analyse duodenal contractility in adult dystrophic (mdx) mice. The spontaneous changes of the isometric tension and the responses of longitudinal duodenal muscle to nonadrenergic, noncholinergic (NANC) nerve stimulation and to exogenous drugs were compared between normal and mdx mice. Duodenal segments from mdx mice displayed spontaneous contractions with higher frequency than normals. Nω-nitro-L-arginine methyl ester (L-NAME) increased the frequency of contractions in normals without affecting that in mdx mice. In normals, NANC nerve stimulation elicited a transient relaxation abolished by L-NAME. In mdx mice a frank relaxation was not observed, the inhibitory response consisted just in the suppression of the phasic activity. This response was reduced by L-NAME and abolished by the subsequent addition of α-chymotrypsin. In normals, α-chymo-trypsin hardly affected NANC relaxation, whilst it significantly antagonised that in mdx mice. Mdx duodenal muscle also showed a reduced responsiveness to sodium nitroprusside, and to 8-bromoguanosine 3′, 5′-cyclic monophosphate in comparison with normal preparations. The results indicate that mdx mice experience duodenal contractile disturbances due to an impairment of NO function with defective responsiveness of the muscle to NO. The reduction in NO influence is functionally compensated by the peptidergic system.",
keywords = "Dystrophin, Gastroenterology, Inhibitory pathways, Intestinal relaxation, Neuroscience (all), Nitric oxide, Physiology, Smooth muscle, mdx mice",
author = "Zizzo, {Maria Grazia} and Flavia Mule' and Serio, {Rosa Maria}",
year = "2003",
language = "English",
volume = "15",
pages = "559--565",
journal = "Neurogastroenterology and Motility",
issn = "1350-1925",
publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Duodenal contractile activity in dystrophic (mdx) mice: Reduction of nitric oxide influence

AU - Zizzo, Maria Grazia

AU - Mule', Flavia

AU - Serio, Rosa Maria

PY - 2003

Y1 - 2003

N2 - The present study was undertaken to analyse duodenal contractility in adult dystrophic (mdx) mice. The spontaneous changes of the isometric tension and the responses of longitudinal duodenal muscle to nonadrenergic, noncholinergic (NANC) nerve stimulation and to exogenous drugs were compared between normal and mdx mice. Duodenal segments from mdx mice displayed spontaneous contractions with higher frequency than normals. Nω-nitro-L-arginine methyl ester (L-NAME) increased the frequency of contractions in normals without affecting that in mdx mice. In normals, NANC nerve stimulation elicited a transient relaxation abolished by L-NAME. In mdx mice a frank relaxation was not observed, the inhibitory response consisted just in the suppression of the phasic activity. This response was reduced by L-NAME and abolished by the subsequent addition of α-chymotrypsin. In normals, α-chymo-trypsin hardly affected NANC relaxation, whilst it significantly antagonised that in mdx mice. Mdx duodenal muscle also showed a reduced responsiveness to sodium nitroprusside, and to 8-bromoguanosine 3′, 5′-cyclic monophosphate in comparison with normal preparations. The results indicate that mdx mice experience duodenal contractile disturbances due to an impairment of NO function with defective responsiveness of the muscle to NO. The reduction in NO influence is functionally compensated by the peptidergic system.

AB - The present study was undertaken to analyse duodenal contractility in adult dystrophic (mdx) mice. The spontaneous changes of the isometric tension and the responses of longitudinal duodenal muscle to nonadrenergic, noncholinergic (NANC) nerve stimulation and to exogenous drugs were compared between normal and mdx mice. Duodenal segments from mdx mice displayed spontaneous contractions with higher frequency than normals. Nω-nitro-L-arginine methyl ester (L-NAME) increased the frequency of contractions in normals without affecting that in mdx mice. In normals, NANC nerve stimulation elicited a transient relaxation abolished by L-NAME. In mdx mice a frank relaxation was not observed, the inhibitory response consisted just in the suppression of the phasic activity. This response was reduced by L-NAME and abolished by the subsequent addition of α-chymotrypsin. In normals, α-chymo-trypsin hardly affected NANC relaxation, whilst it significantly antagonised that in mdx mice. Mdx duodenal muscle also showed a reduced responsiveness to sodium nitroprusside, and to 8-bromoguanosine 3′, 5′-cyclic monophosphate in comparison with normal preparations. The results indicate that mdx mice experience duodenal contractile disturbances due to an impairment of NO function with defective responsiveness of the muscle to NO. The reduction in NO influence is functionally compensated by the peptidergic system.

KW - Dystrophin

KW - Gastroenterology

KW - Inhibitory pathways

KW - Intestinal relaxation

KW - Neuroscience (all)

KW - Nitric oxide

KW - Physiology

KW - Smooth muscle

KW - mdx mice

UR - http://hdl.handle.net/10447/199030

M3 - Article

VL - 15

SP - 559

EP - 565

JO - Neurogastroenterology and Motility

JF - Neurogastroenterology and Motility

SN - 1350-1925

ER -