Draft genome of a novel methanotrophic Methylobacter sp. from the volcanic soils of Pantelleria Island

Antonina Lisa Gagliano, Paola Quatrini, Jeroen Frank, Antonia L. Gagliano, Walter D’Alessandro, Lianna Poghosyan, Carmen Hogendoorn, Nunzia Picone, Femke Van Hout, Sophie Vijverberg, Theo A. Van Alen, Huub J. M. Op Den Camp, Mike S. M. Jetten, Arjan Pol

Risultato della ricerca: Articlepeer review

2 Citazioni (Scopus)

Abstract

The genus Methylobacter is considered an important and often dominant group of aerobic methane-oxidizing bacteria in many oxic ecosystems, where members of this genus contribute to the reduction of CH4 emissions. Metagenomic studies of the upper oxic layers of geothermal soils of the Favara Grande, Pantelleria, Italy, revealed the presence of various methane-oxidizing bacteria, and resulted in a near complete metagenome assembled genome (MAG) of an aerobic methanotroph, which was classified as a Methylobacter species. In this study, the Methylobacter sp. B2 MAG was used to investigate its metabolic potential and phylogenetic affiliation. The MAG has a size of 4,086,539 bp, consists of 134 contigs and 3955 genes were found, of which 3902 were protein coding genes. All genes for CH4 oxidation to CO2 were detected, including pmoCAB encoding particulate methane monooxygenase (pMMO) and xoxF encoding a methanol dehydrogenase. No gene encoding a formaldehyde dehydrogenase was present and the formaldehyde to formate conversion follows the tetrahydromethanopterin (H4MPT) pathway. “Ca. Methylobacter favarea” B2 uses the Ribulose-Mono-Phosphate (RuMP) pathway for carbon fixation. Analysis of the MAG indicates that Na+/H+ antiporters and the urease system might be important in the maintenance of pH homeostasis of this strain to cope with acidic conditions. So far, thermoacidophilic Methylobacter species have not been isolated, however this study indicates that members of the genus Methylobacter can be found in distinct ecosystems and their presence is not restricted to freshwater or marine sediments.
Lingua originaleEnglish
pagine (da-a)313-324
Numero di pagine12
RivistaAntonie van Leeuwenhoek
Volume114
Stato di pubblicazionePublished - 2021

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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