Downregulation of miRNA17-92 cluster marks Vγ9Vδ2 T cells from patients with rheumatoid arthritis

Giuliana Guggino, Francesco Dieli, Riccardo Alessandro, Laura Saieva, Francesco Ciccia, Marco Pio La Manna, Nadia Rosalia Caccamo, Marco Pio La Manna, Valentina Orlando, Piero Ruscitti, Francesco Dieli, Nadia Caccamo, Roberto Giacomelli, Paola Cipriani, Valentina Orlando

Risultato della ricerca: Article

1 Citazione (Scopus)

Abstract

Background: We aimed to evaluate the phenotype, function, and microRNA (miRNA)17-92 cluster expression in Vγ9Vδ2 T-cell subsets and the correlation with immune response in rheumatoid arthritis (RA) patients. Methods: Peripheral blood from 10 early RA untreated patients and 10 healthy donors (HD) was obtained. Polyclonal Vγ9Vδ2 T-cell lines were generated and analysed by flow cytometry. Analysis of miRNA17-92 cluster expression was performed by real-time polymerase chain reaction (RT-PCR), and expression of mRNA target genes was also studied. Results: A remarkable change in the distribution of Vγ9Vδ2 T-cell functional subsets was observed in the peripheral blood of RA patients compared with HD, with an expansion of effector subsets and reduction of naive cells which was accompanied by modifications in proinflammatory cytokine expression. Vγ9Vδ2 T cells with a TEM (effector memory) phenotype and producing proinflammatory cytokines were correlated with disease activity score (DAS28). The comparison of miRNA expression among Vγ9Vδ2 T-cell subsets from RA patients and HD showed a lower level of miR-106a-5p and miR-20a-5p, and a higher level of miR-21a-5p, among Vγ9Vδ2 TEM cells, and a lower level of miR-19b-3p among Vγ9Vδ2 TCM (central memory) cells was also found. These differentially expressed miRNAs correlated with higher levels of expression of interleukin (IL)-8, IL-6, and PDCD4 genes. Conclusions: Our results provide evidence for a role of miR-106a, miR-19-3p, miR-20a, and miR-21a in the regulation of Vγ9Vδ2 T-cell function in RA patients and suggest the possibility that the miRNA17-92 family and Vγ9Vδ2 T cells contribute to the pathogenesis of RA.
Lingua originaleEnglish
pagine (da-a)236-
Numero di pagine10
RivistaARTHRITIS RESEARCH & THERAPY
Volume20
Stato di pubblicazionePublished - 2018

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Rheumatoid Arthritis
Down-Regulation
T-Lymphocytes
T-Lymphocyte Subsets
MicroRNAs
Tissue Donors
Cytokines
Phenotype
Interleukin-8
Genes
Real-Time Polymerase Chain Reaction
Interleukin-6
Flow Cytometry
Cell Line
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology and Allergy
  • Immunology

Cita questo

Downregulation of miRNA17-92 cluster marks Vγ9Vδ2 T cells from patients with rheumatoid arthritis. / Guggino, Giuliana; Dieli, Francesco; Alessandro, Riccardo; Saieva, Laura; Ciccia, Francesco; La Manna, Marco Pio; Caccamo, Nadia Rosalia; La Manna, Marco Pio; Orlando, Valentina; Ruscitti, Piero; Dieli, Francesco; Caccamo, Nadia; Giacomelli, Roberto; Cipriani, Paola; Orlando, Valentina.

In: ARTHRITIS RESEARCH & THERAPY, Vol. 20, 2018, pag. 236-.

Risultato della ricerca: Article

Guggino, Giuliana ; Dieli, Francesco ; Alessandro, Riccardo ; Saieva, Laura ; Ciccia, Francesco ; La Manna, Marco Pio ; Caccamo, Nadia Rosalia ; La Manna, Marco Pio ; Orlando, Valentina ; Ruscitti, Piero ; Dieli, Francesco ; Caccamo, Nadia ; Giacomelli, Roberto ; Cipriani, Paola ; Orlando, Valentina. / Downregulation of miRNA17-92 cluster marks Vγ9Vδ2 T cells from patients with rheumatoid arthritis. In: ARTHRITIS RESEARCH & THERAPY. 2018 ; Vol. 20. pagg. 236-.
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title = "Downregulation of miRNA17-92 cluster marks Vγ9Vδ2 T cells from patients with rheumatoid arthritis",
abstract = "Background: We aimed to evaluate the phenotype, function, and microRNA (miRNA)17-92 cluster expression in Vγ9Vδ2 T-cell subsets and the correlation with immune response in rheumatoid arthritis (RA) patients. Methods: Peripheral blood from 10 early RA untreated patients and 10 healthy donors (HD) was obtained. Polyclonal Vγ9Vδ2 T-cell lines were generated and analysed by flow cytometry. Analysis of miRNA17-92 cluster expression was performed by real-time polymerase chain reaction (RT-PCR), and expression of mRNA target genes was also studied. Results: A remarkable change in the distribution of Vγ9Vδ2 T-cell functional subsets was observed in the peripheral blood of RA patients compared with HD, with an expansion of effector subsets and reduction of naive cells which was accompanied by modifications in proinflammatory cytokine expression. Vγ9Vδ2 T cells with a TEM (effector memory) phenotype and producing proinflammatory cytokines were correlated with disease activity score (DAS28). The comparison of miRNA expression among Vγ9Vδ2 T-cell subsets from RA patients and HD showed a lower level of miR-106a-5p and miR-20a-5p, and a higher level of miR-21a-5p, among Vγ9Vδ2 TEM cells, and a lower level of miR-19b-3p among Vγ9Vδ2 TCM (central memory) cells was also found. These differentially expressed miRNAs correlated with higher levels of expression of interleukin (IL)-8, IL-6, and PDCD4 genes. Conclusions: Our results provide evidence for a role of miR-106a, miR-19-3p, miR-20a, and miR-21a in the regulation of Vγ9Vδ2 T-cell function in RA patients and suggest the possibility that the miRNA17-92 family and Vγ9Vδ2 T cells contribute to the pathogenesis of RA.",
keywords = "Immunology, Immunology and Allergy, Inflammatory cytokines, Rheumatoid arthritis, Rheumatology, miRNA17-92, γδ T cells",
author = "Giuliana Guggino and Francesco Dieli and Riccardo Alessandro and Laura Saieva and Francesco Ciccia and {La Manna}, {Marco Pio} and Caccamo, {Nadia Rosalia} and {La Manna}, {Marco Pio} and Valentina Orlando and Piero Ruscitti and Francesco Dieli and Nadia Caccamo and Roberto Giacomelli and Paola Cipriani and Valentina Orlando",
year = "2018",
language = "English",
volume = "20",
pages = "236--",
journal = "ARTHRITIS RESEARCH & THERAPY",
issn = "1478-6354",

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TY - JOUR

T1 - Downregulation of miRNA17-92 cluster marks Vγ9Vδ2 T cells from patients with rheumatoid arthritis

AU - Guggino, Giuliana

AU - Dieli, Francesco

AU - Alessandro, Riccardo

AU - Saieva, Laura

AU - Ciccia, Francesco

AU - La Manna, Marco Pio

AU - Caccamo, Nadia Rosalia

AU - La Manna, Marco Pio

AU - Orlando, Valentina

AU - Ruscitti, Piero

AU - Dieli, Francesco

AU - Caccamo, Nadia

AU - Giacomelli, Roberto

AU - Cipriani, Paola

AU - Orlando, Valentina

PY - 2018

Y1 - 2018

N2 - Background: We aimed to evaluate the phenotype, function, and microRNA (miRNA)17-92 cluster expression in Vγ9Vδ2 T-cell subsets and the correlation with immune response in rheumatoid arthritis (RA) patients. Methods: Peripheral blood from 10 early RA untreated patients and 10 healthy donors (HD) was obtained. Polyclonal Vγ9Vδ2 T-cell lines were generated and analysed by flow cytometry. Analysis of miRNA17-92 cluster expression was performed by real-time polymerase chain reaction (RT-PCR), and expression of mRNA target genes was also studied. Results: A remarkable change in the distribution of Vγ9Vδ2 T-cell functional subsets was observed in the peripheral blood of RA patients compared with HD, with an expansion of effector subsets and reduction of naive cells which was accompanied by modifications in proinflammatory cytokine expression. Vγ9Vδ2 T cells with a TEM (effector memory) phenotype and producing proinflammatory cytokines were correlated with disease activity score (DAS28). The comparison of miRNA expression among Vγ9Vδ2 T-cell subsets from RA patients and HD showed a lower level of miR-106a-5p and miR-20a-5p, and a higher level of miR-21a-5p, among Vγ9Vδ2 TEM cells, and a lower level of miR-19b-3p among Vγ9Vδ2 TCM (central memory) cells was also found. These differentially expressed miRNAs correlated with higher levels of expression of interleukin (IL)-8, IL-6, and PDCD4 genes. Conclusions: Our results provide evidence for a role of miR-106a, miR-19-3p, miR-20a, and miR-21a in the regulation of Vγ9Vδ2 T-cell function in RA patients and suggest the possibility that the miRNA17-92 family and Vγ9Vδ2 T cells contribute to the pathogenesis of RA.

AB - Background: We aimed to evaluate the phenotype, function, and microRNA (miRNA)17-92 cluster expression in Vγ9Vδ2 T-cell subsets and the correlation with immune response in rheumatoid arthritis (RA) patients. Methods: Peripheral blood from 10 early RA untreated patients and 10 healthy donors (HD) was obtained. Polyclonal Vγ9Vδ2 T-cell lines were generated and analysed by flow cytometry. Analysis of miRNA17-92 cluster expression was performed by real-time polymerase chain reaction (RT-PCR), and expression of mRNA target genes was also studied. Results: A remarkable change in the distribution of Vγ9Vδ2 T-cell functional subsets was observed in the peripheral blood of RA patients compared with HD, with an expansion of effector subsets and reduction of naive cells which was accompanied by modifications in proinflammatory cytokine expression. Vγ9Vδ2 T cells with a TEM (effector memory) phenotype and producing proinflammatory cytokines were correlated with disease activity score (DAS28). The comparison of miRNA expression among Vγ9Vδ2 T-cell subsets from RA patients and HD showed a lower level of miR-106a-5p and miR-20a-5p, and a higher level of miR-21a-5p, among Vγ9Vδ2 TEM cells, and a lower level of miR-19b-3p among Vγ9Vδ2 TCM (central memory) cells was also found. These differentially expressed miRNAs correlated with higher levels of expression of interleukin (IL)-8, IL-6, and PDCD4 genes. Conclusions: Our results provide evidence for a role of miR-106a, miR-19-3p, miR-20a, and miR-21a in the regulation of Vγ9Vδ2 T-cell function in RA patients and suggest the possibility that the miRNA17-92 family and Vγ9Vδ2 T cells contribute to the pathogenesis of RA.

KW - Immunology

KW - Immunology and Allergy

KW - Inflammatory cytokines

KW - Rheumatoid arthritis

KW - Rheumatology

KW - miRNA17-92

KW - γδ T cells

UR - http://hdl.handle.net/10447/358967

UR - http://arthritis-research.com/

M3 - Article

VL - 20

SP - 236-

JO - ARTHRITIS RESEARCH & THERAPY

JF - ARTHRITIS RESEARCH & THERAPY

SN - 1478-6354

ER -