Double negative (CD19(+)IgG(+)IgD(-)CD27(-)) B lymphocytes: A new insight from telomerase in healthy elderly, in centenarian offspring and in Alzheimer's disease patients.

Azzarello, D.

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15 Citazioni (Scopus)

Abstract

t. Immunosenescence is characterized by the impairment of humoral immunity with changes in the mem-ory/naive B cell compartment. In particular we have previously reported the percentage increase of aMemory IgD−CD27−(Double Negative, DN) B cell population in aged people. In this study, we have fur-ther characterized DN B cells with the aim to better understand their contribution to immunosenescence.As DN B cells show a poor ability to proliferate in vitro, we have evaluated the expression of the inhibitoryreceptors CD307d and CD22 on these cells from young and old individuals. In addition we have evalu-ated the ability to activate DN B cells by the simultaneous use of innate (CpG) and adaptive ( -Ig/CD40)ligands. Our data demonstrate that the refractoriness to proliferate of DN B cells does not depend onthe expression of inhibitory receptors, but it is due to the kind of stimulation. Indeed, when DN B cellsare stimulated engaging both BCR and TLR9, they become able to proliferate and reactivate the telome-rase enzyme. In the present study, we have also compared the telomerase activity in a group of peoplegenetically advantaged for longevity as centenarian offspring (CO) and in a group of moderate–severeAlzheimer’s disease (AD) patients, who represent a model of unsuccessful aging. Our study suggests thattelomerase reactivation of DN B cells, as well as their number and ability in activating, depend essentiallyby the biological age of the subjects studied, so the evaluation of DN B cells might allow to gain insightto healthy and unsuccessful aging.
Lingua originaleEnglish
pagine (da-a)303-309
Numero di pagine7
RivistaImmunology Letters
Volume162
Stato di pubblicazionePublished - 2014

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Immunoglobulin D
Telomerase
Alzheimer Disease
B-Lymphocytes
Immunoglobulin G
Aptitude
CD40 Ligand
Humoral Immunity
Enzymes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cita questo

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title = "Double negative (CD19(+)IgG(+)IgD(-)CD27(-)) B lymphocytes: A new insight from telomerase in healthy elderly, in centenarian offspring and in Alzheimer's disease patients.",
abstract = "t. Immunosenescence is characterized by the impairment of humoral immunity with changes in the mem-ory/naive B cell compartment. In particular we have previously reported the percentage increase of aMemory IgD−CD27−(Double Negative, DN) B cell population in aged people. In this study, we have fur-ther characterized DN B cells with the aim to better understand their contribution to immunosenescence.As DN B cells show a poor ability to proliferate in vitro, we have evaluated the expression of the inhibitoryreceptors CD307d and CD22 on these cells from young and old individuals. In addition we have evalu-ated the ability to activate DN B cells by the simultaneous use of innate (CpG) and adaptive ( -Ig/CD40)ligands. Our data demonstrate that the refractoriness to proliferate of DN B cells does not depend onthe expression of inhibitory receptors, but it is due to the kind of stimulation. Indeed, when DN B cellsare stimulated engaging both BCR and TLR9, they become able to proliferate and reactivate the telome-rase enzyme. In the present study, we have also compared the telomerase activity in a group of peoplegenetically advantaged for longevity as centenarian offspring (CO) and in a group of moderate–severeAlzheimer’s disease (AD) patients, who represent a model of unsuccessful aging. Our study suggests thattelomerase reactivation of DN B cells, as well as their number and ability in activating, depend essentiallyby the biological age of the subjects studied, so the evaluation of DN B cells might allow to gain insightto healthy and unsuccessful aging.",
author = "{Azzarello, D.} and Calogero Caruso and {Colonna Romano}, Giuseppina and Cecilia Camarda and Balistreri, {Carmela Rita} and Matteo Bulati and Silvio Buffa and Roberto Monastero and Adriana Martorana",
year = "2014",
language = "English",
volume = "162",
pages = "303--309",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier",

}

TY - JOUR

T1 - Double negative (CD19(+)IgG(+)IgD(-)CD27(-)) B lymphocytes: A new insight from telomerase in healthy elderly, in centenarian offspring and in Alzheimer's disease patients.

AU - Azzarello, D.

AU - Caruso, Calogero

AU - Colonna Romano, Giuseppina

AU - Camarda, Cecilia

AU - Balistreri, Carmela Rita

AU - Bulati, Matteo

AU - Buffa, Silvio

AU - Monastero, Roberto

AU - Martorana, Adriana

PY - 2014

Y1 - 2014

N2 - t. Immunosenescence is characterized by the impairment of humoral immunity with changes in the mem-ory/naive B cell compartment. In particular we have previously reported the percentage increase of aMemory IgD−CD27−(Double Negative, DN) B cell population in aged people. In this study, we have fur-ther characterized DN B cells with the aim to better understand their contribution to immunosenescence.As DN B cells show a poor ability to proliferate in vitro, we have evaluated the expression of the inhibitoryreceptors CD307d and CD22 on these cells from young and old individuals. In addition we have evalu-ated the ability to activate DN B cells by the simultaneous use of innate (CpG) and adaptive ( -Ig/CD40)ligands. Our data demonstrate that the refractoriness to proliferate of DN B cells does not depend onthe expression of inhibitory receptors, but it is due to the kind of stimulation. Indeed, when DN B cellsare stimulated engaging both BCR and TLR9, they become able to proliferate and reactivate the telome-rase enzyme. In the present study, we have also compared the telomerase activity in a group of peoplegenetically advantaged for longevity as centenarian offspring (CO) and in a group of moderate–severeAlzheimer’s disease (AD) patients, who represent a model of unsuccessful aging. Our study suggests thattelomerase reactivation of DN B cells, as well as their number and ability in activating, depend essentiallyby the biological age of the subjects studied, so the evaluation of DN B cells might allow to gain insightto healthy and unsuccessful aging.

AB - t. Immunosenescence is characterized by the impairment of humoral immunity with changes in the mem-ory/naive B cell compartment. In particular we have previously reported the percentage increase of aMemory IgD−CD27−(Double Negative, DN) B cell population in aged people. In this study, we have fur-ther characterized DN B cells with the aim to better understand their contribution to immunosenescence.As DN B cells show a poor ability to proliferate in vitro, we have evaluated the expression of the inhibitoryreceptors CD307d and CD22 on these cells from young and old individuals. In addition we have evalu-ated the ability to activate DN B cells by the simultaneous use of innate (CpG) and adaptive ( -Ig/CD40)ligands. Our data demonstrate that the refractoriness to proliferate of DN B cells does not depend onthe expression of inhibitory receptors, but it is due to the kind of stimulation. Indeed, when DN B cellsare stimulated engaging both BCR and TLR9, they become able to proliferate and reactivate the telome-rase enzyme. In the present study, we have also compared the telomerase activity in a group of peoplegenetically advantaged for longevity as centenarian offspring (CO) and in a group of moderate–severeAlzheimer’s disease (AD) patients, who represent a model of unsuccessful aging. Our study suggests thattelomerase reactivation of DN B cells, as well as their number and ability in activating, depend essentiallyby the biological age of the subjects studied, so the evaluation of DN B cells might allow to gain insightto healthy and unsuccessful aging.

UR - http://hdl.handle.net/10447/99719

M3 - Article

VL - 162

SP - 303

EP - 309

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

ER -