DNA ploidy and S-phase fraction, but not p53 or NM23-H1 expression, predict outcome in colorectal cancer patients. Result of a 5-year prospective study

Sergio Salerno, Nicolo' Gebbia, Antonio Russo, Rossana Porcasi, Rosa Maria Tomasino, Vincenza Morello, Nello Grassi, Maria Rosaria Valerio, Antonella Amato, Fabio Fulfaro, Viviana Bazan, Nicola Gebbia, Russo, Dardanoni, Zanna, Salerno, Simona Corsale, Porcasi, Fabio Fulfaro, Manuela MigliavaccaGerbino, Nuara, Morello, Viviana Bazan, Grassi, Valentina Calò, Dusonchet, Sergio Salerno, Latteri, Maria Rosaria Valerio, Tomasino, Tubiolo, Antonella Amato, Patrizia Cammareri, Carla Tubiolo, Federica Latteri, Ines Zanna

Risultato della ricerca: Articlepeer review

41 Citazioni (Scopus)

Abstract

Purpose: The aim of this study was to determine TP53 and NM23-H1 immunoreactivity, DNA ploidy, and S-phase fraction (SPF) in a series of 160 patients undergoing resective surgery for primary operable colorectal cancer (CRC) and to establish whether these alterations have any clinical value in predicting CRC patients' prognosis. Methods: TP53 and NM23-H1 expressions were evaluated on paraffin-embedded tissue by immunohistochemistry and DNA-ploidy and SPF on frozen tissue by flow-cytometric analysis. Results: The median follow-up time in our study group was 71 months (range 34-115 months). P53 protein expression was associated with distal tumors (P < 0.05) and DNA aneuploid tumors (P < 0.05) tumors. DNA-aneuploidy was associated with distal tumors (P < 0.01), histological grade (G3) (P < 0.05), advanced Dukes' stage (C and D) (P < 0.01), lymph node metastases (P < 0.01) and high SPF (> 18.3%) (P < 0.01). The major significant predictors for both disease relapse and death were advanced Dukes' stage, DNA-aneuploidy, and high SPF, while lymphohematic invasion was the only independent factor for relapse and non-curative resection for death. Conclusions: Our results indicate that DNA aneuploidy and high SPF are associated in CRC with a poor clinical 5-year outcome, while in contrast the prognostic role of TP53 and NM23-H1 expression is still to be clarified.
Lingua originaleEnglish
pagine (da-a)650-658
Numero di pagine9
RivistaJournal of Cancer Research and Clinical Oncology
Volume128
Stato di pubblicazionePublished - 2002

All Science Journal Classification (ASJC) codes

  • ???subjectarea.asjc.2700.2730???
  • ???subjectarea.asjc.1300.1306???

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