We have previously reported a molecular and cytogenetic characterization of three different5S rDNA clusters in the sea urchin Paracentrotus lividus and recently, demonstrated the presence ofhigh heterogeneity in functional 5S rRNA. In this paper, we show some important distinctive data on 5SrRNA transcription for this organism. Using Single Strand Conformation Polymorphism (SSCP)analysis, we demonstrate the existence of two classes of 5S rRNA, one which is embryo-specific andencoded by the smallest (700bp) cluster and the other which is expressed at every stage and encodedby longer clusters (900 and 950bp). We also demonstrate that the embryo-specific class of 5S rRNA isexpressed in oocytes and embryonic stages and is silenced in adult tissue and that this phenomenonappears to be due exclusively to DNA methylation, as indicated by sensitivity to 5-azacytidine, unlikeXenopus where this mechanism is necessary but not sufficient to maintain the silenced status.
|Numero di pagine||6|
|Stato di pubblicazione||Published - 2013|
All Science Journal Classification (ASJC) codes