DNA interaction of CuII, NiII and ZnII functionalized salphen complexes: studies by linear dichroism, gel electrophoresis and PCR

Alessio Terenzi, Giampaolo Barone, Cosimo Ducani, Alessio Terenzi, Michael J. Hannon, Louise Male

Risultato della ricerca: Article

22 Citazioni (Scopus)

Abstract

The interaction of salphen-type NiII, CuII and ZnII complexes with native DNA was investigated by exploiting linear dichroism experiments. The NiII complex behaves as a typical intercalator, binding strongly and stiffening and unwinding the DNA. The strength of the DNA interaction is slightly weaker for the copper complex and much weaker for the zinc complex. Plasmid-DNA gel electrophoresis experiments indicated that while CuII and ZnII complexes do not induce the unwinding of supercoiled DNA, the NiII complex has a nuclease activity without the addition of external agents. On the other hand, as shown in the PCR assays, we demonstrate that, at the used concentrations, only the CuII complex is able to inhibit the DNA amplification mediated by Taq DNA polymerase. In this paper we have also reported a detailed characterization of the three compounds including 2D-NMR and ESI-mass experiments and X-ray single crystal structure of the copper and nickel compounds.
Lingua originaleEnglish
pagine (da-a)11220-11226
Numero di pagine7
RivistaDalton Transactions
Volume42
Stato di pubblicazionePublished - 2013

All Science Journal Classification (ASJC) codes

  • Inorganic Chemistry

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