DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection

Salvatore Feo, Claudia Pellerito, Patrizia Cancemi, Bruno Giuseppe Pignataro, Giuseppe Domenico Arrabito, Hede, Desideri, Ottaviani, Ho, Giovanni Marletta, Vittorio Ferrara, Knudsen, Felicia Cavaleri

Risultato della ricerca: Chapter

Abstract

Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.
Lingua originaleEnglish
Titolo della pubblicazione ospiteLecture Notes in Electrical Engineering
Pagine309-316
Numero di pagine8
Stato di pubblicazionePublished - 2019

Serie di pubblicazioni

NomeLECTURE NOTES IN ELECTRICAL ENGINEERING

Fingerprint

Biosensors
Lithography
DNA
Oligonucleotides
Substrates
Screening
Molecules
Sensors

All Science Journal Classification (ASJC) codes

  • Industrial and Manufacturing Engineering

Cita questo

Feo, S., Pellerito, C., Cancemi, P., Pignataro, B. G., Arrabito, G. D., Hede, ... Cavaleri, F. (2019). DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. In Lecture Notes in Electrical Engineering (pagg. 309-316). (LECTURE NOTES IN ELECTRICAL ENGINEERING).

DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. / Feo, Salvatore; Pellerito, Claudia; Cancemi, Patrizia; Pignataro, Bruno Giuseppe; Arrabito, Giuseppe Domenico; Hede; Desideri; Ottaviani; Ho; Marletta, Giovanni; Ferrara, Vittorio; Knudsen; Cavaleri, Felicia.

Lecture Notes in Electrical Engineering. 2019. pag. 309-316 (LECTURE NOTES IN ELECTRICAL ENGINEERING).

Risultato della ricerca: Chapter

Feo, S, Pellerito, C, Cancemi, P, Pignataro, BG, Arrabito, GD, Hede, Desideri, Ottaviani, Ho, Marletta, G, Ferrara, V, Knudsen & Cavaleri, F 2019, DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. in Lecture Notes in Electrical Engineering. LECTURE NOTES IN ELECTRICAL ENGINEERING, pagg. 309-316.
Feo S, Pellerito C, Cancemi P, Pignataro BG, Arrabito GD, Hede e altri. DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. In Lecture Notes in Electrical Engineering. 2019. pag. 309-316. (LECTURE NOTES IN ELECTRICAL ENGINEERING).
Feo, Salvatore ; Pellerito, Claudia ; Cancemi, Patrizia ; Pignataro, Bruno Giuseppe ; Arrabito, Giuseppe Domenico ; Hede ; Desideri ; Ottaviani ; Ho ; Marletta, Giovanni ; Ferrara, Vittorio ; Knudsen ; Cavaleri, Felicia. / DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. Lecture Notes in Electrical Engineering. 2019. pagg. 309-316 (LECTURE NOTES IN ELECTRICAL ENGINEERING).
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abstract = "Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.",
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AU - Feo, Salvatore

AU - Pellerito, Claudia

AU - Cancemi, Patrizia

AU - Pignataro, Bruno Giuseppe

AU - Arrabito, Giuseppe Domenico

AU - Hede, null

AU - Desideri, null

AU - Ottaviani, null

AU - Ho, null

AU - Marletta, Giovanni

AU - Ferrara, Vittorio

AU - Knudsen, null

AU - Cavaleri, Felicia

PY - 2019

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AB - Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.

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