TY - CHAP
T1 - DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection
AU - Feo, Salvatore
AU - Pellerito, Claudia
AU - Arrabito, Giuseppe Domenico
AU - Pignataro, Bruno Giuseppe
AU - Cancemi, Patrizia
AU - Hede, null
AU - Desideri, null
AU - Ottaviani, null
AU - Ho, null
AU - Marletta, Giovanni
AU - Ferrara, Vittorio
AU - Knudsen, null
AU - Cavaleri, Felicia
PY - 2019
Y1 - 2019
N2 - Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.
AB - Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.
UR - http://hdl.handle.net/10447/346288
UR - https://link.springer.com/chapter/10.1007/978-3-030-04324-7_39#citeas
M3 - Chapter
SN - 978-3-030-04323-0; 978-3-030-04324-7
T3 - LECTURE NOTES IN ELECTRICAL ENGINEERING
SP - 309
EP - 316
BT - Lecture Notes in Electrical Engineering
ER -