Abstract

gd T cells usually infiltrate many different types of cancer, but it is unclear whether they inhibit or promote tumor progression. Moreover, properties of tumor-infiltrating gd T cells and those in the corresponding normal tissue remain largely unknown. Here we have studied features of gd T cells in colorectal cancer, normal colon tissue and peripheral blood, and correlated their levels with clinicopathologic hallmarks. Flow cytometry and transcriptome analyses showed that the tumor comprised a highly variable rate of TILs (5-90%) and 4%gd T cells on average, with the majority expressing Vd1. Most Vd1 and Vd2 T cells showed a predominant effector memory phenotype and had reduced production of IFN-g which was likely due to yet unidentified inhibitory molecules present in cancer stem cell secretome. Transcriptome analyses revealed that patients containing abundant gd T cells had significantly longer 5-year disease free survival rate, suggesting their efficacy in controlling tumor at very early stage.
Lingua originaleEnglish
pagine (da-a)e1347742-
Numero di pagine12
RivistaOncoImmunology
Volume6
Stato di pubblicazionePublished - 2017

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Colorectal Neoplasms
T-Lymphocytes
Neoplasms
Gene Expression Profiling
Neoplastic Stem Cells
Colonic Neoplasms
Disease-Free Survival
Flow Cytometry
Survival Rate
Phenotype

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Oncology

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@article{5770a3f476144752a321a62412617ed3,
title = "Distinctive features of tumor-infiltrating gd T lymphocytes in human colorectal cancer",
abstract = "gd T cells usually infiltrate many different types of cancer, but it is unclear whether they inhibit or promote tumor progression. Moreover, properties of tumor-infiltrating gd T cells and those in the corresponding normal tissue remain largely unknown. Here we have studied features of gd T cells in colorectal cancer, normal colon tissue and peripheral blood, and correlated their levels with clinicopathologic hallmarks. Flow cytometry and transcriptome analyses showed that the tumor comprised a highly variable rate of TILs (5-90{\%}) and 4{\%}gd T cells on average, with the majority expressing Vd1. Most Vd1 and Vd2 T cells showed a predominant effector memory phenotype and had reduced production of IFN-g which was likely due to yet unidentified inhibitory molecules present in cancer stem cell secretome. Transcriptome analyses revealed that patients containing abundant gd T cells had significantly longer 5-year disease free survival rate, suggesting their efficacy in controlling tumor at very early stage.",
author = "Francesco Dieli and Serena Meraviglia and Salvatore Vieni and {La Mendola}, Carmela and {Lo Presti}, Elena and Matilde Todaro and Giuseppe Cicero and Giorgio Stassi and Tosolini and Fourni{\`e}, {Jean Jacques} and Valentina Orlando and Veronica Catalano",
year = "2017",
language = "English",
volume = "6",
pages = "e1347742--",
journal = "OncoImmunology",
issn = "2162-4011",
publisher = "Landes Bioscience",

}

TY - JOUR

T1 - Distinctive features of tumor-infiltrating gd T lymphocytes in human colorectal cancer

AU - Dieli, Francesco

AU - Meraviglia, Serena

AU - Vieni, Salvatore

AU - La Mendola, Carmela

AU - Lo Presti, Elena

AU - Todaro, Matilde

AU - Cicero, Giuseppe

AU - Stassi, Giorgio

AU - Tosolini, null

AU - Fourniè, Jean Jacques

AU - Orlando, Valentina

AU - Catalano, Veronica

PY - 2017

Y1 - 2017

N2 - gd T cells usually infiltrate many different types of cancer, but it is unclear whether they inhibit or promote tumor progression. Moreover, properties of tumor-infiltrating gd T cells and those in the corresponding normal tissue remain largely unknown. Here we have studied features of gd T cells in colorectal cancer, normal colon tissue and peripheral blood, and correlated their levels with clinicopathologic hallmarks. Flow cytometry and transcriptome analyses showed that the tumor comprised a highly variable rate of TILs (5-90%) and 4%gd T cells on average, with the majority expressing Vd1. Most Vd1 and Vd2 T cells showed a predominant effector memory phenotype and had reduced production of IFN-g which was likely due to yet unidentified inhibitory molecules present in cancer stem cell secretome. Transcriptome analyses revealed that patients containing abundant gd T cells had significantly longer 5-year disease free survival rate, suggesting their efficacy in controlling tumor at very early stage.

AB - gd T cells usually infiltrate many different types of cancer, but it is unclear whether they inhibit or promote tumor progression. Moreover, properties of tumor-infiltrating gd T cells and those in the corresponding normal tissue remain largely unknown. Here we have studied features of gd T cells in colorectal cancer, normal colon tissue and peripheral blood, and correlated their levels with clinicopathologic hallmarks. Flow cytometry and transcriptome analyses showed that the tumor comprised a highly variable rate of TILs (5-90%) and 4%gd T cells on average, with the majority expressing Vd1. Most Vd1 and Vd2 T cells showed a predominant effector memory phenotype and had reduced production of IFN-g which was likely due to yet unidentified inhibitory molecules present in cancer stem cell secretome. Transcriptome analyses revealed that patients containing abundant gd T cells had significantly longer 5-year disease free survival rate, suggesting their efficacy in controlling tumor at very early stage.

UR - http://hdl.handle.net/10447/245773

M3 - Article

VL - 6

SP - e1347742-

JO - OncoImmunology

JF - OncoImmunology

SN - 2162-4011

ER -