The cytotoxic effect of several diorganotin(IV) and triorganotin(IV)-meso-tetra(4-sulfonatophenyl)porphine derivatives wastested and only the (Bu2Sn)2TPPS and the (Bu3Sn)4TPPS showed cytotoxicity on A375 human melanoma cells. To examine thepathway of (Bu2Sn)2TPPS or (Bu3Sn)4TPPS induced A375 cell death, DNA fragmentation analysis, Annexin V binding and PIuptake as well as caspases activation analysis by Western blot were carried out. A375 cells treated exhibited several typicalcharacteristics of apoptosis. Both the (Bu2Sn)2TPPS and the (Bu3Sn)4TPPS compounds activate caspase-8 and caspase-9leading to caspase-3 activation. Thus, we propose that these two porphirin derivatives lead to the apoptosis of human melanomacells via both death receptor-mediated and mitochondrial apoptotic pathways.
|Numero di pagine||11|
|Stato di pubblicazione||Published - 2006|
All Science Journal Classification (ASJC) codes