Differentiation and characterization of rat adipose tissue mesenchymal stem cells into endothelial-like cells

Giovanni Cassata, Giuseppa Purpari, Attilio Ignazio Lo Monte, Giuseppe Damiano, Roberta Altomare, Vincenzo Davide Palumbo, Giovanni Cassata, Di Marco, Di Bella, Russotto, Alessandra Santoro, Russo Lacerna, Gucciardi, Giuseppa Purpari, Cannella, Giuseppe Piccione, Annalisa Guercio, Pietro Di Marco

Risultato della ricerca: Article

3 Citazioni (Scopus)

Abstract

In this study, mesenchymal stem cells were isolated from rat adipose tissue (AD-MSCs) to characterize and differentiate them into endothelial-like cells. AD-MSCs were isolated by mechanical and enzymatic treatments, and their identity was verified by colony-forming units (CFU) test and by differentiation into cells of mesodermal lineages. The endothelial differentiation was induced by plating another aliquot of cells in EGM-2 medium, enriched with specific endothelial growth factors. Five subcultures were performed. The expression of stemness genes (OCT4, SOX2 and NANOG) was investigated. The presence of CD90 and the absence of the CD45 were evaluated by flow cytometry. The endothelial-like cells were characterized by the evaluation of morphological changes and gene expression analysis for endothelial markers (CD31, CD144, CD146). Characterization of AD-MSCs showed their ability to form clones, to differentiate in vitro and the OCT-4, SOX-2, NANOG genes expression. Immunophenotypic characterization showed the CD90 presence and the CD45 absence. The endothelial-like cells showed morphological changes, the expression of CD31, CD144, CD146 genes and the presence of CD31 membrane receptor. Matrigel assay showed their ability to form network and vessels-like structures. This study lays the foundations for future evaluation of the potential AD-MSCs pro-angiogenic and therapeutic role.
Lingua originaleEnglish
pagine (da-a)11-20
Numero di pagine10
RivistaANATOMIA HISTOLOGIA EMBRYOLOGIA
Volume47
Stato di pubblicazionePublished - 2018

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Mesenchymal Stromal Cells
Adipose Tissue
Endothelial Cells
Gene Expression
Endothelial Growth Factors
Cell Lineage
Flow Cytometry
Stem Cells
Clone Cells
Membranes
Genes
Therapeutics

All Science Journal Classification (ASJC) codes

  • veterinary(all)

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Differentiation and characterization of rat adipose tissue mesenchymal stem cells into endothelial-like cells. / Cassata, Giovanni; Purpari, Giuseppa; Lo Monte, Attilio Ignazio; Damiano, Giuseppe; Altomare, Roberta; Palumbo, Vincenzo Davide; Cassata, Giovanni; Di Marco; Di Bella; Russotto; Santoro, Alessandra; Russo Lacerna; Gucciardi; Purpari, Giuseppa; Cannella; Piccione, Giuseppe; Guercio, Annalisa; Di Marco, Pietro.

In: ANATOMIA HISTOLOGIA EMBRYOLOGIA, Vol. 47, 2018, pag. 11-20.

Risultato della ricerca: Article

Cassata, G, Purpari, G, Lo Monte, AI, Damiano, G, Altomare, R, Palumbo, VD, Cassata, G, Di Marco, Di Bella, Russotto, Santoro, A, Russo Lacerna, Gucciardi, Purpari, G, Cannella, Piccione, G, Guercio, A & Di Marco, P 2018, 'Differentiation and characterization of rat adipose tissue mesenchymal stem cells into endothelial-like cells', ANATOMIA HISTOLOGIA EMBRYOLOGIA, vol. 47, pagg. 11-20.
Cassata, Giovanni ; Purpari, Giuseppa ; Lo Monte, Attilio Ignazio ; Damiano, Giuseppe ; Altomare, Roberta ; Palumbo, Vincenzo Davide ; Cassata, Giovanni ; Di Marco ; Di Bella ; Russotto ; Santoro, Alessandra ; Russo Lacerna ; Gucciardi ; Purpari, Giuseppa ; Cannella ; Piccione, Giuseppe ; Guercio, Annalisa ; Di Marco, Pietro. / Differentiation and characterization of rat adipose tissue mesenchymal stem cells into endothelial-like cells. In: ANATOMIA HISTOLOGIA EMBRYOLOGIA. 2018 ; Vol. 47. pagg. 11-20.
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abstract = "In this study, mesenchymal stem cells were isolated from rat adipose tissue (AD-MSCs) to characterize and differentiate them into endothelial-like cells. AD-MSCs were isolated by mechanical and enzymatic treatments, and their identity was verified by colony-forming units (CFU) test and by differentiation into cells of mesodermal lineages. The endothelial differentiation was induced by plating another aliquot of cells in EGM-2 medium, enriched with specific endothelial growth factors. Five subcultures were performed. The expression of stemness genes (OCT4, SOX2 and NANOG) was investigated. The presence of CD90 and the absence of the CD45 were evaluated by flow cytometry. The endothelial-like cells were characterized by the evaluation of morphological changes and gene expression analysis for endothelial markers (CD31, CD144, CD146). Characterization of AD-MSCs showed their ability to form clones, to differentiate in vitro and the OCT-4, SOX-2, NANOG genes expression. Immunophenotypic characterization showed the CD90 presence and the CD45 absence. The endothelial-like cells showed morphological changes, the expression of CD31, CD144, CD146 genes and the presence of CD31 membrane receptor. Matrigel assay showed their ability to form network and vessels-like structures. This study lays the foundations for future evaluation of the potential AD-MSCs pro-angiogenic and therapeutic role.",
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AU - Cassata, Giovanni

AU - Purpari, Giuseppa

AU - Lo Monte, Attilio Ignazio

AU - Damiano, Giuseppe

AU - Altomare, Roberta

AU - Palumbo, Vincenzo Davide

AU - Cassata, Giovanni

AU - Di Marco, null

AU - Di Bella, null

AU - Russotto, null

AU - Santoro, Alessandra

AU - Russo Lacerna, null

AU - Gucciardi, null

AU - Purpari, Giuseppa

AU - Cannella, null

AU - Piccione, Giuseppe

AU - Guercio, Annalisa

AU - Di Marco, Pietro

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N2 - In this study, mesenchymal stem cells were isolated from rat adipose tissue (AD-MSCs) to characterize and differentiate them into endothelial-like cells. AD-MSCs were isolated by mechanical and enzymatic treatments, and their identity was verified by colony-forming units (CFU) test and by differentiation into cells of mesodermal lineages. The endothelial differentiation was induced by plating another aliquot of cells in EGM-2 medium, enriched with specific endothelial growth factors. Five subcultures were performed. The expression of stemness genes (OCT4, SOX2 and NANOG) was investigated. The presence of CD90 and the absence of the CD45 were evaluated by flow cytometry. The endothelial-like cells were characterized by the evaluation of morphological changes and gene expression analysis for endothelial markers (CD31, CD144, CD146). Characterization of AD-MSCs showed their ability to form clones, to differentiate in vitro and the OCT-4, SOX-2, NANOG genes expression. Immunophenotypic characterization showed the CD90 presence and the CD45 absence. The endothelial-like cells showed morphological changes, the expression of CD31, CD144, CD146 genes and the presence of CD31 membrane receptor. Matrigel assay showed their ability to form network and vessels-like structures. This study lays the foundations for future evaluation of the potential AD-MSCs pro-angiogenic and therapeutic role.

AB - In this study, mesenchymal stem cells were isolated from rat adipose tissue (AD-MSCs) to characterize and differentiate them into endothelial-like cells. AD-MSCs were isolated by mechanical and enzymatic treatments, and their identity was verified by colony-forming units (CFU) test and by differentiation into cells of mesodermal lineages. The endothelial differentiation was induced by plating another aliquot of cells in EGM-2 medium, enriched with specific endothelial growth factors. Five subcultures were performed. The expression of stemness genes (OCT4, SOX2 and NANOG) was investigated. The presence of CD90 and the absence of the CD45 were evaluated by flow cytometry. The endothelial-like cells were characterized by the evaluation of morphological changes and gene expression analysis for endothelial markers (CD31, CD144, CD146). Characterization of AD-MSCs showed their ability to form clones, to differentiate in vitro and the OCT-4, SOX-2, NANOG genes expression. Immunophenotypic characterization showed the CD90 presence and the CD45 absence. The endothelial-like cells showed morphological changes, the expression of CD31, CD144, CD146 genes and the presence of CD31 membrane receptor. Matrigel assay showed their ability to form network and vessels-like structures. This study lays the foundations for future evaluation of the potential AD-MSCs pro-angiogenic and therapeutic role.

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