TY - JOUR
T1 - Differential expression of two glucocorticoid receptors in seabass (teleost fish) head kidney after exogeneous cortisol inoculation
AU - Celi, Monica
AU - Vizzini, Aiti
AU - Sanfratello, Maria Antonietta
AU - Parrinello, Nicolo'
AU - Salerno, Giuseppina
AU - Vazzana, Mirella
PY - 2010
Y1 - 2010
N2 - Stressful conditions include a prompt release of corticosteroid hormones which can mediate gene expression through glucocorticoid receptors (GR). Since two seabass (Dicentrarchus labrax) GRs have been cloned and sequenced from peritoneal cavity cells (DlGR1) and liver (DlGR2), a comparative amino acid sequence analysis that included Haplochromis burtoni HbGRs, was carried out and homologies disclosed. The DlGR1 and DlGR2 deduced aminoacid sequences showed 61% identity (I) and 70% similarity (S). Moreover, DlGR2 was similar to HbGR2b (69% I, 73% S), and the DlGR1 to HbGR1 (72% I, 78% S). In addition, we examined the expression of the DlGRs after exogeneous cortisol inoculation into the peritoneal cavity, mimicking stresseffects. At various times after the administration (3 h, 24 h, 1 week), gene expressions was evaluated in headkidney by real-time PCR. In addition, immunoblotting and densitometry analyses were performed with anti- DlGR1 antibodies. Although sea bass head kidney expressed both DlGR1 and DlGR2 they were differentially modulated by intraperitoneal implant of exogeneous cortisol.
AB - Stressful conditions include a prompt release of corticosteroid hormones which can mediate gene expression through glucocorticoid receptors (GR). Since two seabass (Dicentrarchus labrax) GRs have been cloned and sequenced from peritoneal cavity cells (DlGR1) and liver (DlGR2), a comparative amino acid sequence analysis that included Haplochromis burtoni HbGRs, was carried out and homologies disclosed. The DlGR1 and DlGR2 deduced aminoacid sequences showed 61% identity (I) and 70% similarity (S). Moreover, DlGR2 was similar to HbGR2b (69% I, 73% S), and the DlGR1 to HbGR1 (72% I, 78% S). In addition, we examined the expression of the DlGRs after exogeneous cortisol inoculation into the peritoneal cavity, mimicking stresseffects. At various times after the administration (3 h, 24 h, 1 week), gene expressions was evaluated in headkidney by real-time PCR. In addition, immunoblotting and densitometry analyses were performed with anti- DlGR1 antibodies. Although sea bass head kidney expressed both DlGR1 and DlGR2 they were differentially modulated by intraperitoneal implant of exogeneous cortisol.
KW - Cortisol
KW - Dicentrarchus labrax
KW - Glucocorticoid receptor
KW - Real-time PCR
KW - Cortisol
KW - Dicentrarchus labrax
KW - Glucocorticoid receptor
KW - Real-time PCR
UR - http://hdl.handle.net/10447/51413
M3 - Article
VL - 157
SP - 49
EP - 54
JO - COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART A, MOLECULAR & INTEGRATIVE PHYSIOLOGY
JF - COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART A, MOLECULAR & INTEGRATIVE PHYSIOLOGY
SN - 1095-6433
ER -