Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex.

    Risultato della ricerca: Article

    Abstract

    A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria
    Lingua originaleEnglish
    Numero di pagine3
    RivistaIranian Journal of Microbiology
    Volume4
    Stato di pubblicazionePublished - 2012

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