Development of a modified DNA extraction method for pulsed-field gelelectrophoresis analysis of Staphylococcus aureus and enterococci withoutusing lysostaphin

Arash Ghodousi; Maryam Omrani; Arash Ghodousi; Fereshteh Shahcheraghi; Mohammad Mehdi Feizabadi; Bizhan Nomanpour

Development of a modified DNA extraction method for pulsed-field gelelectrophoresis analysis of Staphylococcus aureus and enterococci withoutusing lysostaphin

Arash Ghodousi, Maryam Omrani, Arash Ghodousi, Fereshteh Shahcheraghi, Mohammad Mehdi Feizabadi, Bizhan Nomanpour

Risultato della ricerca: Article › peer review

Abstract

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates ofStaphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces theexpenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during thespheroplast formation from these bacteria.

Lingua originale	English
Numero di pagine	4
Rivista	Journal of Microbiological Methods
Volume	84
Stato di pubblicazione	Published - 2011

All Science Journal Classification (ASJC) codes

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???subjectarea.asjc.1300.1312???
???subjectarea.asjc.2700.2726???

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title = "Development of a modified DNA extraction method for pulsed-field gelelectrophoresis analysis of Staphylococcus aureus and enterococci withoutusing lysostaphin",

abstract = "A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates ofStaphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces theexpenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during thespheroplast formation from these bacteria.",

author = "Arash Ghodousi and Maryam Omrani and Arash Ghodousi and Fereshteh Shahcheraghi and Feizabadi, {Mohammad Mehdi} and Bizhan Nomanpour",

year = "2011",

language = "English",

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journal = "Journal of Microbiological Methods",

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T1 - Development of a modified DNA extraction method for pulsed-field gelelectrophoresis analysis of Staphylococcus aureus and enterococci withoutusing lysostaphin

AU - Ghodousi, Arash

AU - Omrani, Maryam

AU - Ghodousi, Arash

AU - Shahcheraghi, Fereshteh

AU - Feizabadi, Mohammad Mehdi

AU - Nomanpour, Bizhan

PY - 2011

Y1 - 2011

N2 - A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates ofStaphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces theexpenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during thespheroplast formation from these bacteria.

AB - A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates ofStaphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces theexpenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during thespheroplast formation from these bacteria.

UR - http://hdl.handle.net/10447/77582

UR - http://www.sciencedirect.com/science/article/pii/S0167701210003805

M3 - Article

SN - 0167-7012

VL - 84

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

Development of a modified DNA extraction method for pulsed-field gelelectrophoresis analysis of Staphylococcus aureus and enterococci withoutusing lysostaphin

Abstract

All Science Journal Classification (ASJC) codes

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