Development of a modified DNA extraction method for pulsed-field gelelectrophoresis analysis of Staphylococcus aureus and enterococci withoutusing lysostaphin

Arash Ghodousi, Maryam Omrani, Arash Ghodousi, Fereshteh Shahcheraghi, Mohammad Mehdi Feizabadi, Bizhan Nomanpour

    Risultato della ricerca: Article

    6 Citazioni (Scopus)

    Abstract

    A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates ofStaphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces theexpenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during thespheroplast formation from these bacteria.
    Lingua originaleEnglish
    Numero di pagine4
    RivistaJournal of Microbiological Methods
    Volume84
    Stato di pubblicazionePublished - 2011

    All Science Journal Classification (ASJC) codes

    • Microbiology
    • Molecular Biology
    • Microbiology (medical)

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