Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain

Paolo Procaccianti, Antonina Argo, Paolo Procaccianti, Lucia Dell'Acqua, Antonella Argo, Gabriella Roda, Veniero Gambaro, Fiorenza Farè, Katia Guerrini, Daria Catalano, Cristina Borroni

Risultato della ricerca: Article

11 Citazioni (Scopus)

Abstract

Brain distribution pattern of “street” heroin metabolites (morphine and codeine) was investigated in two fatalities due to “acute narcotism”. A suitable sample pretreatment prior to solid-phase-extraction was developed to achieve a good recovery of the analytes and to eliminate the interfering species. After derivatization with MSTFA, samples were analyzed by GC/MS. Specificity, accuracy, precision and linearity of the method were evaluated; LOD and LOQ were, respectively, 10 ng/25 ng for morphine and 5 ng/10 ng for codeine. This method was applied to the analysis of six brain areas (hippocampus, frontal lobe, occipital lobe, nuclei, bulb and pons) coming from two cases of heroin-related deaths. No evidence of accumulation of metabolites in a specific brain region was found
Lingua originaleEnglish
pagine (da-a)125-130
Numero di pagine6
RivistaJournal of Pharmaceutical and Biomedical Analysis
Volume73
Stato di pubblicazionePublished - 2012

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Opiate Alkaloids
Metabolites
Codeine
Brain
Heroin
Morphine
Occipital Lobe
Pons
Frontal Lobe
Hippocampus
Recovery

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Clinical Biochemistry
  • Spectroscopy
  • Drug Discovery
  • Pharmaceutical Science

Cita questo

Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain. / Procaccianti, Paolo; Argo, Antonina; Procaccianti, Paolo; Dell'Acqua, Lucia; Argo, Antonella; Roda, Gabriella; Gambaro, Veniero; Farè, Fiorenza; Guerrini, Katia; Catalano, Daria; Borroni, Cristina.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 73, 2012, pag. 125-130.

Risultato della ricerca: Article

Procaccianti, P, Argo, A, Procaccianti, P, Dell'Acqua, L, Argo, A, Roda, G, Gambaro, V, Farè, F, Guerrini, K, Catalano, D & Borroni, C 2012, 'Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain', Journal of Pharmaceutical and Biomedical Analysis, vol. 73, pagg. 125-130.
Procaccianti, Paolo ; Argo, Antonina ; Procaccianti, Paolo ; Dell'Acqua, Lucia ; Argo, Antonella ; Roda, Gabriella ; Gambaro, Veniero ; Farè, Fiorenza ; Guerrini, Katia ; Catalano, Daria ; Borroni, Cristina. / Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain. In: Journal of Pharmaceutical and Biomedical Analysis. 2012 ; Vol. 73. pagg. 125-130.
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abstract = "Brain distribution pattern of “street” heroin metabolites (morphine and codeine) was investigated in two fatalities due to “acute narcotism”. A suitable sample pretreatment prior to solid-phase-extraction was developed to achieve a good recovery of the analytes and to eliminate the interfering species. After derivatization with MSTFA, samples were analyzed by GC/MS. Specificity, accuracy, precision and linearity of the method were evaluated; LOD and LOQ were, respectively, 10 ng/25 ng for morphine and 5 ng/10 ng for codeine. This method was applied to the analysis of six brain areas (hippocampus, frontal lobe, occipital lobe, nuclei, bulb and pons) coming from two cases of heroin-related deaths. No evidence of accumulation of metabolites in a specific brain region was found",
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AU - Procaccianti, Paolo

AU - Argo, Antonina

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AU - Dell'Acqua, Lucia

AU - Argo, Antonella

AU - Roda, Gabriella

AU - Gambaro, Veniero

AU - Farè, Fiorenza

AU - Guerrini, Katia

AU - Catalano, Daria

AU - Borroni, Cristina

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