Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain

Antonina Argo, Paolo Procaccianti, Katia Guerrini, Daria Catalano, Cristina Borroni, Antonella Argo, Paolo Procaccianti, Lucia Dell'Acqua, Gabriella Roda, Veniero Gambaro, Fiorenza Farè

Risultato della ricerca: Article

11 Citazioni (Scopus)

Abstract

Brain distribution pattern of “street” heroin metabolites (morphine and codeine) was investigated intwo fatalities due to “acute narcotism”. A suitable sample pretreatment prior to solid-phase-extractionwas developed to achieve a good recovery of the analytes and to eliminate the interfering species. Afterderivatization with MSTFA, samples were analyzed by GC/MS. Specificity, accuracy, precision and linearityof the method were evaluated; LOD and LOQ were, respectively, 10 ng/25 ng for morphine and 5 ng/10 ngfor codeine.This method was applied to the analysis of six brain areas (hippocampus, frontal lobe, occipital lobe, nuclei, bulb and pons) coming from two cases of heroin-related deaths. No evidence of accumulation of metabolites in a specific brain region was found
Lingua originaleEnglish
pagine (da-a)125-130
Numero di pagine6
RivistaJournal of Pharmaceutical and Biomedical Analysis
Volume73
Stato di pubblicazionePublished - 2012

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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    Argo, A., Procaccianti, P., Guerrini, K., Catalano, D., Borroni, C., Argo, A., Procaccianti, P., Dell'Acqua, L., Roda, G., Gambaro, V., & Farè, F. (2012). Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain. Journal of Pharmaceutical and Biomedical Analysis, 73, 125-130.