Determination of ketosteroid hormones in meat by liquid chromatography tandem mass spectrometry and derivatization chemistry

Serena Indelicato, Donatella Aiello, Domenico Taverna, Hicham Benabdelkamel, Anna Napoli, Fabio Mazzotti, Leonardo Di Donna, Fabio Mazzotti, Giovanni Sindona

Risultato della ricerca: Article

25 Citazioni (Scopus)

Abstract

A method for the determination and quantification of ketosteroid hormones in meat by mass spectrometry, based on the derivatization of the carbonyl moiety of steroids by Omethylhydroxylamine, is presented. The quantitative assay is performed by means of multiple-reaction-monitoring (MRM) scan mode and using the corresponding labelled species, obtained by reaction with d3-methoxylamine, as internal standard. The accuracy of the method was established by evaluating artificially spiked samples, obtaining values in the range 90-110 %. Recovery tests were performed on blank matrix samples spiked with non-natural steroids including trenbolone and melengestrol acetate. The latter experiment revealed that the yield of the extraction processes was approximately 60 %. Good values of LOQ and LOD were achieved, making this method competitive with current hormone assay methods.
Lingua originaleEnglish
pagine (da-a)5835-5842
Numero di pagine8
RivistaAnalytical and Bioanalytical Chemistry
Volume407
Stato di pubblicazionePublished - 2015

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

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