Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and individual carriers by real time PCR

Arash Ghodousi, Mohammad Mehdi Feizabadi, Soroor Asadi, Bizhan Nomanpour, Toraj Babaei, Seyd Alijavad Mousavi, Arash Ghodousi

    Risultato della ricerca: Article

    3 Citazioni (Scopus)

    Abstract

    The aim of this study was to develop a real time polymerase chain reaction (PCR) for quantitative detection of Streptococcus pneumoniae from clinical respiratory specimens. Initially, 184 respiratory specimens from patients with community acquired pneumonia (CAP) (n = 129) and 55 cases with hospital associated pneumonia (HAP) were bacteriologically investigated. To check the colonization status among the healthy individuals, 32 preschool and 31 adults were screened in parallel. All specimens were cultured on selective culture media to isolate S. pneumoniae, Legionella spp. and Mycoplasma spp. A 166 bp fragment corresponding to cbp A gene of S. pneumoniae was amplified from clinical specimens using Taqman probe real time PCR. Culture showed 14, but real time PCR showed 15 specimens as being positive for S. pneumoniae. The specificity and sensitivity of real time PCR was 99.14% and 100 respectively. Co-infections of S. pneumoniae with Legionella pneumophila, Chlamydophila pneumoniae, Mycoplasma pneumoniae and Staphylococcus aureus were observed in 5 cases (35.72%). S. pneumoniae was counted <103 cfu/ml from the co-infected cases. Using real time PCR, a cutoff of 103cfu/ml is introduced to differentiate colonization from infection in respiratory tract. This is the first report on the prevalence CAP with S. pneumoniae in Iran (12.40%)
    Lingua originaleEnglish
    pagine (da-a)-
    Numero di pagine7
    RivistaAfrican Journal of Biotechnology
    Volume10
    Stato di pubblicazionePublished - 2011

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    Streptococcus pneumoniae
    pneumonia
    Real-Time Polymerase Chain Reaction
    Pneumonia
    quantitative polymerase chain reaction
    Mycoplasma pneumoniae
    Chlamydophila pneumoniae
    Legionella pneumophila
    Legionella
    Mycoplasma
    Iran
    Coinfection
    mixed infection
    Respiratory Tract Infections
    respiratory tract diseases
    Culture Media
    Staphylococcus aureus
    culture media
    Sensitivity and Specificity
    Genes

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Agronomy and Crop Science
    • Genetics
    • Molecular Biology
    • Applied Microbiology and Biotechnology

    Cita questo

    Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and individual carriers by real time PCR. / Ghodousi, Arash; Feizabadi, Mohammad Mehdi; Asadi, Soroor; Nomanpour, Bizhan; Babaei, Toraj; Mousavi, Seyd Alijavad; Ghodousi, Arash.

    In: African Journal of Biotechnology, Vol. 10, 2011, pag. -.

    Risultato della ricerca: Article

    Ghodousi, Arash ; Feizabadi, Mohammad Mehdi ; Asadi, Soroor ; Nomanpour, Bizhan ; Babaei, Toraj ; Mousavi, Seyd Alijavad ; Ghodousi, Arash. / Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and individual carriers by real time PCR. In: African Journal of Biotechnology. 2011 ; Vol. 10. pagg. -.
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    title = "Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and individual carriers by real time PCR",
    abstract = "The aim of this study was to develop a real time polymerase chain reaction (PCR) for quantitative detection of Streptococcus pneumoniae from clinical respiratory specimens. Initially, 184 respiratory specimens from patients with community acquired pneumonia (CAP) (n = 129) and 55 cases with hospital associated pneumonia (HAP) were bacteriologically investigated. To check the colonization status among the healthy individuals, 32 preschool and 31 adults were screened in parallel. All specimens were cultured on selective culture media to isolate S. pneumoniae, Legionella spp. and Mycoplasma spp. A 166 bp fragment corresponding to cbp A gene of S. pneumoniae was amplified from clinical specimens using Taqman probe real time PCR. Culture showed 14, but real time PCR showed 15 specimens as being positive for S. pneumoniae. The specificity and sensitivity of real time PCR was 99.14{\%} and 100 respectively. Co-infections of S. pneumoniae with Legionella pneumophila, Chlamydophila pneumoniae, Mycoplasma pneumoniae and Staphylococcus aureus were observed in 5 cases (35.72{\%}). S. pneumoniae was counted <103 cfu/ml from the co-infected cases. Using real time PCR, a cutoff of 103cfu/ml is introduced to differentiate colonization from infection in respiratory tract. This is the first report on the prevalence CAP with S. pneumoniae in Iran (12.40{\%})",
    author = "Arash Ghodousi and Feizabadi, {Mohammad Mehdi} and Soroor Asadi and Bizhan Nomanpour and Toraj Babaei and Mousavi, {Seyd Alijavad} and Arash Ghodousi",
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    AU - Ghodousi, Arash

    AU - Feizabadi, Mohammad Mehdi

    AU - Asadi, Soroor

    AU - Nomanpour, Bizhan

    AU - Babaei, Toraj

    AU - Mousavi, Seyd Alijavad

    AU - Ghodousi, Arash

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    N2 - The aim of this study was to develop a real time polymerase chain reaction (PCR) for quantitative detection of Streptococcus pneumoniae from clinical respiratory specimens. Initially, 184 respiratory specimens from patients with community acquired pneumonia (CAP) (n = 129) and 55 cases with hospital associated pneumonia (HAP) were bacteriologically investigated. To check the colonization status among the healthy individuals, 32 preschool and 31 adults were screened in parallel. All specimens were cultured on selective culture media to isolate S. pneumoniae, Legionella spp. and Mycoplasma spp. A 166 bp fragment corresponding to cbp A gene of S. pneumoniae was amplified from clinical specimens using Taqman probe real time PCR. Culture showed 14, but real time PCR showed 15 specimens as being positive for S. pneumoniae. The specificity and sensitivity of real time PCR was 99.14% and 100 respectively. Co-infections of S. pneumoniae with Legionella pneumophila, Chlamydophila pneumoniae, Mycoplasma pneumoniae and Staphylococcus aureus were observed in 5 cases (35.72%). S. pneumoniae was counted <103 cfu/ml from the co-infected cases. Using real time PCR, a cutoff of 103cfu/ml is introduced to differentiate colonization from infection in respiratory tract. This is the first report on the prevalence CAP with S. pneumoniae in Iran (12.40%)

    AB - The aim of this study was to develop a real time polymerase chain reaction (PCR) for quantitative detection of Streptococcus pneumoniae from clinical respiratory specimens. Initially, 184 respiratory specimens from patients with community acquired pneumonia (CAP) (n = 129) and 55 cases with hospital associated pneumonia (HAP) were bacteriologically investigated. To check the colonization status among the healthy individuals, 32 preschool and 31 adults were screened in parallel. All specimens were cultured on selective culture media to isolate S. pneumoniae, Legionella spp. and Mycoplasma spp. A 166 bp fragment corresponding to cbp A gene of S. pneumoniae was amplified from clinical specimens using Taqman probe real time PCR. Culture showed 14, but real time PCR showed 15 specimens as being positive for S. pneumoniae. The specificity and sensitivity of real time PCR was 99.14% and 100 respectively. Co-infections of S. pneumoniae with Legionella pneumophila, Chlamydophila pneumoniae, Mycoplasma pneumoniae and Staphylococcus aureus were observed in 5 cases (35.72%). S. pneumoniae was counted <103 cfu/ml from the co-infected cases. Using real time PCR, a cutoff of 103cfu/ml is introduced to differentiate colonization from infection in respiratory tract. This is the first report on the prevalence CAP with S. pneumoniae in Iran (12.40%)

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