Data concerning the proteolytic resistance and oxidative stress in LAN5 cells after treatment with BSA hydrogels

Peres, C.; Contardi, M.; Giacomazza, D.

Risultato della ricerca: Article

1 Citazione (Scopus)

Abstract

Proteolytic resistance is a relevant aspect to be tested in the formulation of new nanoscale biomaterials. The action of proteolytic enzymes is a very fast process occurring in the range of few minutes. Here, we report data concerning the proteolytic resistance of a heat-set BSA hydrogel obtained after 20-hour incubation at 60 °C prepared at the pH value of 3.9, pH at which the hydrogel presents the highest elastic character with respect to gel formed at pH 5.9 and 7.4 "Heat-and pH-induced BSA conformational changes, hydrogel formation and application as 3D cell scaffold" (G. Navarra, C. Peres, M. Contardi, P. Picone, P.L. San Biagio, M. Di Carlo, D. Giacomazza, V. Militello, 2016) [1]. We show that the BSA hydrogel produced by heating treatment is protected by the action of proteinase K enzyme. Moreover, we show that LAN5 cells cultured in presence of BSA hydrogels formed at pH 3.9, 5.9 and 7.4 did not exhibit any oxidative stress, one of the first and crucial events causing cell death "Are oxidative stress and mitochondrial dysfunction the key players in the neurodegenerative diseases?" (M. Di Carlo, D. Giacomazza, P. Picone, D. Nuzzo, P.L. San Biagio, 2012) [2] "Effect of zinc oxide nanomaterials induced oxidative stress on the p53 pathway" (M.I. Setyawati, C.Y. Tay, D.T. Leaong, 2013) [3].
Lingua originaleEnglish
pagine (da-a)324-327
Numero di pagine4
RivistaData in Brief
Volume9
Stato di pubblicazionePublished - 2016

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Hydrogels
Hydrogel
Oxidative Stress
Hot Temperature
Zinc Oxide
Endopeptidase K
Gastrin-Secreting Cells
Nanostructures
Biocompatible Materials
Neurodegenerative Diseases
Heating
Cultured Cells
Peptide Hydrolases
Cell Death
Gels
Enzymes

All Science Journal Classification (ASJC) codes

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Data concerning the proteolytic resistance and oxidative stress in LAN5 cells after treatment with BSA hydrogels. / Peres, C.; Contardi, M.; Giacomazza, D.

In: Data in Brief, Vol. 9, 2016, pag. 324-327.

Risultato della ricerca: Article

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title = "Data concerning the proteolytic resistance and oxidative stress in LAN5 cells after treatment with BSA hydrogels",
abstract = "Proteolytic resistance is a relevant aspect to be tested in the formulation of new nanoscale biomaterials. The action of proteolytic enzymes is a very fast process occurring in the range of few minutes. Here, we report data concerning the proteolytic resistance of a heat-set BSA hydrogel obtained after 20-hour incubation at 60 °C prepared at the pH value of 3.9, pH at which the hydrogel presents the highest elastic character with respect to gel formed at pH 5.9 and 7.4 {"}Heat-and pH-induced BSA conformational changes, hydrogel formation and application as 3D cell scaffold{"} (G. Navarra, C. Peres, M. Contardi, P. Picone, P.L. San Biagio, M. Di Carlo, D. Giacomazza, V. Militello, 2016) [1]. We show that the BSA hydrogel produced by heating treatment is protected by the action of proteinase K enzyme. Moreover, we show that LAN5 cells cultured in presence of BSA hydrogels formed at pH 3.9, 5.9 and 7.4 did not exhibit any oxidative stress, one of the first and crucial events causing cell death {"}Are oxidative stress and mitochondrial dysfunction the key players in the neurodegenerative diseases?{"} (M. Di Carlo, D. Giacomazza, P. Picone, D. Nuzzo, P.L. San Biagio, 2012) [2] {"}Effect of zinc oxide nanomaterials induced oxidative stress on the p53 pathway{"} (M.I. Setyawati, C.Y. Tay, D.T. Leaong, 2013) [3].",
keywords = "Cell-scaffold; Drug delivery; Hydrogels; Oxidative Stress; Proteolytic resistance; β-aggregates",
author = "{Peres, C.; Contardi, M.; Giacomazza, D.} and Valeria Militello and Giovanna Navarra and Pasquale Picone and {San Biagio}, {Pier Luigi} and {Di Carlo}, Marta",
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T1 - Data concerning the proteolytic resistance and oxidative stress in LAN5 cells after treatment with BSA hydrogels

AU - Peres, C.; Contardi, M.; Giacomazza, D.

AU - Militello, Valeria

AU - Navarra, Giovanna

AU - Picone, Pasquale

AU - San Biagio, Pier Luigi

AU - Di Carlo, Marta

PY - 2016

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N2 - Proteolytic resistance is a relevant aspect to be tested in the formulation of new nanoscale biomaterials. The action of proteolytic enzymes is a very fast process occurring in the range of few minutes. Here, we report data concerning the proteolytic resistance of a heat-set BSA hydrogel obtained after 20-hour incubation at 60 °C prepared at the pH value of 3.9, pH at which the hydrogel presents the highest elastic character with respect to gel formed at pH 5.9 and 7.4 "Heat-and pH-induced BSA conformational changes, hydrogel formation and application as 3D cell scaffold" (G. Navarra, C. Peres, M. Contardi, P. Picone, P.L. San Biagio, M. Di Carlo, D. Giacomazza, V. Militello, 2016) [1]. We show that the BSA hydrogel produced by heating treatment is protected by the action of proteinase K enzyme. Moreover, we show that LAN5 cells cultured in presence of BSA hydrogels formed at pH 3.9, 5.9 and 7.4 did not exhibit any oxidative stress, one of the first and crucial events causing cell death "Are oxidative stress and mitochondrial dysfunction the key players in the neurodegenerative diseases?" (M. Di Carlo, D. Giacomazza, P. Picone, D. Nuzzo, P.L. San Biagio, 2012) [2] "Effect of zinc oxide nanomaterials induced oxidative stress on the p53 pathway" (M.I. Setyawati, C.Y. Tay, D.T. Leaong, 2013) [3].

AB - Proteolytic resistance is a relevant aspect to be tested in the formulation of new nanoscale biomaterials. The action of proteolytic enzymes is a very fast process occurring in the range of few minutes. Here, we report data concerning the proteolytic resistance of a heat-set BSA hydrogel obtained after 20-hour incubation at 60 °C prepared at the pH value of 3.9, pH at which the hydrogel presents the highest elastic character with respect to gel formed at pH 5.9 and 7.4 "Heat-and pH-induced BSA conformational changes, hydrogel formation and application as 3D cell scaffold" (G. Navarra, C. Peres, M. Contardi, P. Picone, P.L. San Biagio, M. Di Carlo, D. Giacomazza, V. Militello, 2016) [1]. We show that the BSA hydrogel produced by heating treatment is protected by the action of proteinase K enzyme. Moreover, we show that LAN5 cells cultured in presence of BSA hydrogels formed at pH 3.9, 5.9 and 7.4 did not exhibit any oxidative stress, one of the first and crucial events causing cell death "Are oxidative stress and mitochondrial dysfunction the key players in the neurodegenerative diseases?" (M. Di Carlo, D. Giacomazza, P. Picone, D. Nuzzo, P.L. San Biagio, 2012) [2] "Effect of zinc oxide nanomaterials induced oxidative stress on the p53 pathway" (M.I. Setyawati, C.Y. Tay, D.T. Leaong, 2013) [3].

KW - Cell-scaffold; Drug delivery; Hydrogels; Oxidative Stress; Proteolytic resistance; β-aggregates

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UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030333/

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EP - 327

JO - Data in Brief

JF - Data in Brief

SN - 2352-3409

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