Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 324-327 |
| Numero di pagine | 4 |
| Rivista | Data in Brief |
| Volume | 9 |
| Stato di pubblicazione | Published - 2016 |
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Data concerning the proteolytic resistance and oxidative stress in LAN5 cells after treatment with BSA hydrogels. / Peres, C.; Contardi, M.; Giacomazza, D.
In: Data in Brief, Vol. 9, 2016, pag. 324-327.Risultato della ricerca: Article
}
TY - JOUR
T1 - Data concerning the proteolytic resistance and oxidative stress in LAN5 cells after treatment with BSA hydrogels
AU - Peres, C.; Contardi, M.; Giacomazza, D.
AU - Militello, Valeria
AU - Navarra, Giovanna
AU - Picone, Pasquale
AU - San Biagio, Pier Luigi
AU - Di Carlo, Marta
PY - 2016
Y1 - 2016
N2 - Proteolytic resistance is a relevant aspect to be tested in the formulation of new nanoscale biomaterials. The action of proteolytic enzymes is a very fast process occurring in the range of few minutes. Here, we report data concerning the proteolytic resistance of a heat-set BSA hydrogel obtained after 20-hour incubation at 60 °C prepared at the pH value of 3.9, pH at which the hydrogel presents the highest elastic character with respect to gel formed at pH 5.9 and 7.4 "Heat-and pH-induced BSA conformational changes, hydrogel formation and application as 3D cell scaffold" (G. Navarra, C. Peres, M. Contardi, P. Picone, P.L. San Biagio, M. Di Carlo, D. Giacomazza, V. Militello, 2016) [1]. We show that the BSA hydrogel produced by heating treatment is protected by the action of proteinase K enzyme. Moreover, we show that LAN5 cells cultured in presence of BSA hydrogels formed at pH 3.9, 5.9 and 7.4 did not exhibit any oxidative stress, one of the first and crucial events causing cell death "Are oxidative stress and mitochondrial dysfunction the key players in the neurodegenerative diseases?" (M. Di Carlo, D. Giacomazza, P. Picone, D. Nuzzo, P.L. San Biagio, 2012) [2] "Effect of zinc oxide nanomaterials induced oxidative stress on the p53 pathway" (M.I. Setyawati, C.Y. Tay, D.T. Leaong, 2013) [3].
AB - Proteolytic resistance is a relevant aspect to be tested in the formulation of new nanoscale biomaterials. The action of proteolytic enzymes is a very fast process occurring in the range of few minutes. Here, we report data concerning the proteolytic resistance of a heat-set BSA hydrogel obtained after 20-hour incubation at 60 °C prepared at the pH value of 3.9, pH at which the hydrogel presents the highest elastic character with respect to gel formed at pH 5.9 and 7.4 "Heat-and pH-induced BSA conformational changes, hydrogel formation and application as 3D cell scaffold" (G. Navarra, C. Peres, M. Contardi, P. Picone, P.L. San Biagio, M. Di Carlo, D. Giacomazza, V. Militello, 2016) [1]. We show that the BSA hydrogel produced by heating treatment is protected by the action of proteinase K enzyme. Moreover, we show that LAN5 cells cultured in presence of BSA hydrogels formed at pH 3.9, 5.9 and 7.4 did not exhibit any oxidative stress, one of the first and crucial events causing cell death "Are oxidative stress and mitochondrial dysfunction the key players in the neurodegenerative diseases?" (M. Di Carlo, D. Giacomazza, P. Picone, D. Nuzzo, P.L. San Biagio, 2012) [2] "Effect of zinc oxide nanomaterials induced oxidative stress on the p53 pathway" (M.I. Setyawati, C.Y. Tay, D.T. Leaong, 2013) [3].
KW - Cell-scaffold; Drug delivery; Hydrogels; Oxidative Stress; Proteolytic resistance; β-aggregates
UR - http://hdl.handle.net/10447/227558
UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030333/
M3 - Article
VL - 9
SP - 324
EP - 327
JO - Data in Brief
JF - Data in Brief
SN - 2352-3409
ER -