Cytotoxic activity of Holothuria tubulosa (Echinodermata) coelomocytes

Risultato della ricerca: Article

5 Citazioni (Scopus)

Abstract

The immune system of marine invertebrates, in particular that of holothurians, still requires further study. Our research showed that coelomocyte cells contained in the coelomic fluid of the sea cucumber, Holothuria tubulosa, are able to lyse, in vitro, red blood cells in rabbits and sheep. A plaque-forming assay showed spherule cells to be the effector cells, able to release cytotoxic molecules after xenogenic cell contact. The coelomocyte lysate supernatant, analysed by polyacrylamide gel electrophoresis overlay technique, using rabbit and sheep erythrocytes, showed two different haemolytic protein patterns: one calcium dependent and the other calcium independent. The fractions of each pattern were resolved on a polyacrylamide gel and calcium-dependent and independent coelomocyte lysate patterns were compared.
Lingua originaleEnglish
pagine (da-a)334-341
Numero di pagine8
RivistaFISH AND SHELLFISH IMMUNOLOGY
Volume72
Stato di pubblicazionePublished - 2018

Fingerprint

Holothuria
Echinodermata
calcium
Calcium
sheep
gel
Sheep
spherule
erythrocytes
Erythrocytes
Immune system
rabbits
Sea Cucumbers
lysis
immune system
cells
Rabbits
Electrophoresis
Holothuroidea
Assays

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cita questo

@article{5c7fad1c4632488084158c4aa98cf6a6,
title = "Cytotoxic activity of Holothuria tubulosa (Echinodermata) coelomocytes",
abstract = "The immune system of marine invertebrates, in particular that of holothurians, still requires further study. Our research showed that coelomocyte cells contained in the coelomic fluid of the sea cucumber, Holothuria tubulosa, are able to lyse, in vitro, red blood cells in rabbits and sheep. A plaque-forming assay showed spherule cells to be the effector cells, able to release cytotoxic molecules after xenogenic cell contact. The coelomocyte lysate supernatant, analysed by polyacrylamide gel electrophoresis overlay technique, using rabbit and sheep erythrocytes, showed two different haemolytic protein patterns: one calcium dependent and the other calcium independent. The fractions of each pattern were resolved on a polyacrylamide gel and calcium-dependent and independent coelomocyte lysate patterns were compared.",
keywords = "Aquatic Science, Coelomocytes, Environmental Chemistry, Haemolytic activity, Holothuria tubulosa, Lysis plaque assay, Overlay assay",
author = "Mirella Vazzana and Monica Celi and Luigi Inguglia and Debora Russo and Marco Chiaramonte and Vincenzo Arizza and Vincenzo Ferrantelli and Vincenzo Ferrantelli",
year = "2018",
language = "English",
volume = "72",
pages = "334--341",
journal = "FISH AND SHELLFISH IMMUNOLOGY",
issn = "1050-4648",

}

TY - JOUR

T1 - Cytotoxic activity of Holothuria tubulosa (Echinodermata) coelomocytes

AU - Vazzana, Mirella

AU - Celi, Monica

AU - Inguglia, Luigi

AU - Russo, Debora

AU - Chiaramonte, Marco

AU - Arizza, Vincenzo

AU - Ferrantelli, Vincenzo

AU - Ferrantelli, Vincenzo

PY - 2018

Y1 - 2018

N2 - The immune system of marine invertebrates, in particular that of holothurians, still requires further study. Our research showed that coelomocyte cells contained in the coelomic fluid of the sea cucumber, Holothuria tubulosa, are able to lyse, in vitro, red blood cells in rabbits and sheep. A plaque-forming assay showed spherule cells to be the effector cells, able to release cytotoxic molecules after xenogenic cell contact. The coelomocyte lysate supernatant, analysed by polyacrylamide gel electrophoresis overlay technique, using rabbit and sheep erythrocytes, showed two different haemolytic protein patterns: one calcium dependent and the other calcium independent. The fractions of each pattern were resolved on a polyacrylamide gel and calcium-dependent and independent coelomocyte lysate patterns were compared.

AB - The immune system of marine invertebrates, in particular that of holothurians, still requires further study. Our research showed that coelomocyte cells contained in the coelomic fluid of the sea cucumber, Holothuria tubulosa, are able to lyse, in vitro, red blood cells in rabbits and sheep. A plaque-forming assay showed spherule cells to be the effector cells, able to release cytotoxic molecules after xenogenic cell contact. The coelomocyte lysate supernatant, analysed by polyacrylamide gel electrophoresis overlay technique, using rabbit and sheep erythrocytes, showed two different haemolytic protein patterns: one calcium dependent and the other calcium independent. The fractions of each pattern were resolved on a polyacrylamide gel and calcium-dependent and independent coelomocyte lysate patterns were compared.

KW - Aquatic Science

KW - Coelomocytes

KW - Environmental Chemistry

KW - Haemolytic activity

KW - Holothuria tubulosa

KW - Lysis plaque assay

KW - Overlay assay

UR - http://hdl.handle.net/10447/264370

UR - http://www.elsevier.com/inca/publications/store/6/2/2/8/3/2/index.htt

M3 - Article

VL - 72

SP - 334

EP - 341

JO - FISH AND SHELLFISH IMMUNOLOGY

JF - FISH AND SHELLFISH IMMUNOLOGY

SN - 1050-4648

ER -