Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 334-341 |
| Numero di pagine | 8 |
| Rivista | FISH AND SHELLFISH IMMUNOLOGY |
| Volume | 72 |
| Stato di pubblicazione | Published - 2018 |
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All Science Journal Classification (ASJC) codes
- Environmental Chemistry
- Aquatic Science
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Cytotoxic activity of Holothuria tubulosa (Echinodermata) coelomocytes. / Vazzana, Mirella; Celi, Monica; Inguglia, Luigi; Russo, Debora; Chiaramonte, Marco; Arizza, Vincenzo; Ferrantelli, Vincenzo; Ferrantelli, Vincenzo.
In: FISH AND SHELLFISH IMMUNOLOGY, Vol. 72, 2018, pag. 334-341.Risultato della ricerca: Article
}
TY - JOUR
T1 - Cytotoxic activity of Holothuria tubulosa (Echinodermata) coelomocytes
AU - Vazzana, Mirella
AU - Celi, Monica
AU - Inguglia, Luigi
AU - Russo, Debora
AU - Chiaramonte, Marco
AU - Arizza, Vincenzo
AU - Ferrantelli, Vincenzo
AU - Ferrantelli, Vincenzo
PY - 2018
Y1 - 2018
N2 - The immune system of marine invertebrates, in particular that of holothurians, still requires further study. Our research showed that coelomocyte cells contained in the coelomic fluid of the sea cucumber, Holothuria tubulosa, are able to lyse, in vitro, red blood cells in rabbits and sheep. A plaque-forming assay showed spherule cells to be the effector cells, able to release cytotoxic molecules after xenogenic cell contact. The coelomocyte lysate supernatant, analysed by polyacrylamide gel electrophoresis overlay technique, using rabbit and sheep erythrocytes, showed two different haemolytic protein patterns: one calcium dependent and the other calcium independent. The fractions of each pattern were resolved on a polyacrylamide gel and calcium-dependent and independent coelomocyte lysate patterns were compared.
AB - The immune system of marine invertebrates, in particular that of holothurians, still requires further study. Our research showed that coelomocyte cells contained in the coelomic fluid of the sea cucumber, Holothuria tubulosa, are able to lyse, in vitro, red blood cells in rabbits and sheep. A plaque-forming assay showed spherule cells to be the effector cells, able to release cytotoxic molecules after xenogenic cell contact. The coelomocyte lysate supernatant, analysed by polyacrylamide gel electrophoresis overlay technique, using rabbit and sheep erythrocytes, showed two different haemolytic protein patterns: one calcium dependent and the other calcium independent. The fractions of each pattern were resolved on a polyacrylamide gel and calcium-dependent and independent coelomocyte lysate patterns were compared.
KW - Aquatic Science
KW - Coelomocytes
KW - Environmental Chemistry
KW - Haemolytic activity
KW - Holothuria tubulosa
KW - Lysis plaque assay
KW - Overlay assay
UR - http://hdl.handle.net/10447/264370
UR - http://www.elsevier.com/inca/publications/store/6/2/2/8/3/2/index.htt
M3 - Article
VL - 72
SP - 334
EP - 341
JO - FISH AND SHELLFISH IMMUNOLOGY
JF - FISH AND SHELLFISH IMMUNOLOGY
SN - 1050-4648
ER -