Cytological and molecular characterization of three gametoclones of Citrus clementina

Maria Germana', Patrick Ollitrault, François Luro, Claire T. Federici, Fred G. Gmitter Jr, Manuel Talon, Mikeal L. Roose, José Juárez, Gabino Ríos, Chunxian Chen, Marcos A. Machado, Dominique Dambier, Yann Froelicher, Gilles Costantino, Pablo Aleza, Esther Carrera, Miguel A. Naranjo, Victoria Ibáñez, Qiang Xu, Xiuxin Deng & 3 others Wenwu Guo, Kevin Kwok, Luis Navarro

Risultato della ricerca: Article

11 Citazioni (Scopus)

Abstract

Abstract Background Three gametoclonal plants of Citrus clementina Hort. ex Tan., cv. Nules, designated ESP, FRA, and ITA (derived from three labs in Spain, France, and Italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. The study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow cytometry, SSR marker genotyping, and array-Comparative Genomic Hybridization (arrayCGH). Results Chromosome counting and flow cytometry revealed that ESP and FRA were haploid, but ITA was tri-haploid. Homozygous patterns, represented by a single peak (allele), were observed among the three plants at almost all SSR loci distributed across the entire diploid donor genome. Those few loci with extra peaks visualized as output from automated sequencing runs, generally low or ambiguous, might result from amplicons of paralogous members at the locus, non-specific sites, or unexpected recombinant alleles. No new alleles were found, suggesting the genomes remained stable and intact during gametogenesis and regeneration. The integrity of the haploid genome also was supported by array-CGH studies, in which genomic profiles were comparable to the diploid control. Conclusions The presence of few gene hybridization abnormalities, corroborated by gene dosage measurements, were hypothetically due to the segregation of hemizygous alleles and minor genomic rearrangements occurring during the haploidization procedure. In conclusion, these plants that are valuable genetic and breeding materials contain completely homozygous and essentially intact genomes.
Lingua originaleEnglish
pagine (da-a)-
Numero di pagine21
RivistaBMC Plant Biology
Volume13
Stato di pubblicazionePublished - 2013

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Citrus clementina
genome
haploidy
gene dosage
alleles
genomics
loci
flow cytometry
diploidy
gene segregation
comparative genomic hybridization
chromosomes
gametogenesis
homozygosity
ploidy
genotyping
hybridization
France
Italy
Spain

All Science Journal Classification (ASJC) codes

  • Plant Science

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Germana', M., Ollitrault, P., Luro, F., Federici, C. T., Gmitter Jr, F. G., Talon, M., ... Navarro, L. (2013). Cytological and molecular characterization of three gametoclones of Citrus clementina. BMC Plant Biology, 13, -.

Cytological and molecular characterization of three gametoclones of Citrus clementina. / Germana', Maria; Ollitrault, Patrick; Luro, François; Federici, Claire T.; Gmitter Jr, Fred G.; Talon, Manuel; Roose, Mikeal L.; Juárez, José; Ríos, Gabino; Chen, Chunxian; Machado, Marcos A.; Dambier, Dominique; Froelicher, Yann; Costantino, Gilles; Aleza, Pablo; Carrera, Esther; Naranjo, Miguel A.; Ibáñez, Victoria; Xu, Qiang; Deng, Xiuxin; Guo, Wenwu; Kwok, Kevin; Navarro, Luis.

In: BMC Plant Biology, Vol. 13, 2013, pag. -.

Risultato della ricerca: Article

Germana', M, Ollitrault, P, Luro, F, Federici, CT, Gmitter Jr, FG, Talon, M, Roose, ML, Juárez, J, Ríos, G, Chen, C, Machado, MA, Dambier, D, Froelicher, Y, Costantino, G, Aleza, P, Carrera, E, Naranjo, MA, Ibáñez, V, Xu, Q, Deng, X, Guo, W, Kwok, K & Navarro, L 2013, 'Cytological and molecular characterization of three gametoclones of Citrus clementina', BMC Plant Biology, vol. 13, pagg. -.
Germana' M, Ollitrault P, Luro F, Federici CT, Gmitter Jr FG, Talon M e altri. Cytological and molecular characterization of three gametoclones of Citrus clementina. BMC Plant Biology. 2013;13:-.
Germana', Maria ; Ollitrault, Patrick ; Luro, François ; Federici, Claire T. ; Gmitter Jr, Fred G. ; Talon, Manuel ; Roose, Mikeal L. ; Juárez, José ; Ríos, Gabino ; Chen, Chunxian ; Machado, Marcos A. ; Dambier, Dominique ; Froelicher, Yann ; Costantino, Gilles ; Aleza, Pablo ; Carrera, Esther ; Naranjo, Miguel A. ; Ibáñez, Victoria ; Xu, Qiang ; Deng, Xiuxin ; Guo, Wenwu ; Kwok, Kevin ; Navarro, Luis. / Cytological and molecular characterization of three gametoclones of Citrus clementina. In: BMC Plant Biology. 2013 ; Vol. 13. pagg. -.
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title = "Cytological and molecular characterization of three gametoclones of Citrus clementina",
abstract = "Abstract Background Three gametoclonal plants of Citrus clementina Hort. ex Tan., cv. Nules, designated ESP, FRA, and ITA (derived from three labs in Spain, France, and Italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. The study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow cytometry, SSR marker genotyping, and array-Comparative Genomic Hybridization (arrayCGH). Results Chromosome counting and flow cytometry revealed that ESP and FRA were haploid, but ITA was tri-haploid. Homozygous patterns, represented by a single peak (allele), were observed among the three plants at almost all SSR loci distributed across the entire diploid donor genome. Those few loci with extra peaks visualized as output from automated sequencing runs, generally low or ambiguous, might result from amplicons of paralogous members at the locus, non-specific sites, or unexpected recombinant alleles. No new alleles were found, suggesting the genomes remained stable and intact during gametogenesis and regeneration. The integrity of the haploid genome also was supported by array-CGH studies, in which genomic profiles were comparable to the diploid control. Conclusions The presence of few gene hybridization abnormalities, corroborated by gene dosage measurements, were hypothetically due to the segregation of hemizygous alleles and minor genomic rearrangements occurring during the haploidization procedure. In conclusion, these plants that are valuable genetic and breeding materials contain completely homozygous and essentially intact genomes.",
author = "Maria Germana' and Patrick Ollitrault and Fran{\cc}ois Luro and Federici, {Claire T.} and {Gmitter Jr}, {Fred G.} and Manuel Talon and Roose, {Mikeal L.} and Jos{\'e} Ju{\'a}rez and Gabino R{\'i}os and Chunxian Chen and Machado, {Marcos A.} and Dominique Dambier and Yann Froelicher and Gilles Costantino and Pablo Aleza and Esther Carrera and Naranjo, {Miguel A.} and Victoria Ib{\'a}{\~n}ez and Qiang Xu and Xiuxin Deng and Wenwu Guo and Kevin Kwok and Luis Navarro",
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TY - JOUR

T1 - Cytological and molecular characterization of three gametoclones of Citrus clementina

AU - Germana', Maria

AU - Ollitrault, Patrick

AU - Luro, François

AU - Federici, Claire T.

AU - Gmitter Jr, Fred G.

AU - Talon, Manuel

AU - Roose, Mikeal L.

AU - Juárez, José

AU - Ríos, Gabino

AU - Chen, Chunxian

AU - Machado, Marcos A.

AU - Dambier, Dominique

AU - Froelicher, Yann

AU - Costantino, Gilles

AU - Aleza, Pablo

AU - Carrera, Esther

AU - Naranjo, Miguel A.

AU - Ibáñez, Victoria

AU - Xu, Qiang

AU - Deng, Xiuxin

AU - Guo, Wenwu

AU - Kwok, Kevin

AU - Navarro, Luis

PY - 2013

Y1 - 2013

N2 - Abstract Background Three gametoclonal plants of Citrus clementina Hort. ex Tan., cv. Nules, designated ESP, FRA, and ITA (derived from three labs in Spain, France, and Italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. The study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow cytometry, SSR marker genotyping, and array-Comparative Genomic Hybridization (arrayCGH). Results Chromosome counting and flow cytometry revealed that ESP and FRA were haploid, but ITA was tri-haploid. Homozygous patterns, represented by a single peak (allele), were observed among the three plants at almost all SSR loci distributed across the entire diploid donor genome. Those few loci with extra peaks visualized as output from automated sequencing runs, generally low or ambiguous, might result from amplicons of paralogous members at the locus, non-specific sites, or unexpected recombinant alleles. No new alleles were found, suggesting the genomes remained stable and intact during gametogenesis and regeneration. The integrity of the haploid genome also was supported by array-CGH studies, in which genomic profiles were comparable to the diploid control. Conclusions The presence of few gene hybridization abnormalities, corroborated by gene dosage measurements, were hypothetically due to the segregation of hemizygous alleles and minor genomic rearrangements occurring during the haploidization procedure. In conclusion, these plants that are valuable genetic and breeding materials contain completely homozygous and essentially intact genomes.

AB - Abstract Background Three gametoclonal plants of Citrus clementina Hort. ex Tan., cv. Nules, designated ESP, FRA, and ITA (derived from three labs in Spain, France, and Italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. The study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow cytometry, SSR marker genotyping, and array-Comparative Genomic Hybridization (arrayCGH). Results Chromosome counting and flow cytometry revealed that ESP and FRA were haploid, but ITA was tri-haploid. Homozygous patterns, represented by a single peak (allele), were observed among the three plants at almost all SSR loci distributed across the entire diploid donor genome. Those few loci with extra peaks visualized as output from automated sequencing runs, generally low or ambiguous, might result from amplicons of paralogous members at the locus, non-specific sites, or unexpected recombinant alleles. No new alleles were found, suggesting the genomes remained stable and intact during gametogenesis and regeneration. The integrity of the haploid genome also was supported by array-CGH studies, in which genomic profiles were comparable to the diploid control. Conclusions The presence of few gene hybridization abnormalities, corroborated by gene dosage measurements, were hypothetically due to the segregation of hemizygous alleles and minor genomic rearrangements occurring during the haploidization procedure. In conclusion, these plants that are valuable genetic and breeding materials contain completely homozygous and essentially intact genomes.

UR - http://hdl.handle.net/10447/84088

M3 - Article

VL - 13

SP - -

JO - BMC Plant Biology

JF - BMC Plant Biology

SN - 1471-2229

ER -