INTRODUCTION & OBJECTIVES: Cytokines may be involved in the pathogenesis of the Peyronie's disease (PD). Cytokines levels in tunicaalbuginea (TA) specimens were measured by Real Time PCR in PD patients and in control group.MATERIAL & METHODS: Between January 2009 and December2010 20 PD patients affected by PD and 8 patients affected by congenital recurvatum penis (control group) undergoing surgery wereentered in the study. Routine histological examination andmeasurement by Real Time PCR of the expression of the encoding genes for IL-2, IL-4, IL-6, IL-10, IL-13, TGF-β1, TNF-α, IFN-γ andMetalloproteinase (MMP-2) were performed. For the normalization of data, GAPDH (glucerldehyde-3 fosfatehydrogenase) gene normally expressed in all cells was adopted. The analysis of the data wasperformed using the comparative method of Δct. The target gene expression were reported with the fold of induction (FOI) methodcalculated according to GAPDH both in PD and in control Group samples.RESULTS: The absence of inflammatory cells was demonstrated by routine histological examination in all samples. A lower median level of gene expression cytokines was detected in the PD patients when compared with the control group: IL-10 0,3864 (range 0- 1,1483), IL-20,089 (range 0- 0,3586), IL-6 0,0406 (range 0-0,1478), IFN-γ 0,029 (range 0-0,1187), MMP-2 0,2491 (range 0- 0,6480). IL-4, IL-13 and TNF-α were not detectable. No significant difference emerged between PD and control patients, although a trend for higher levels of TGF-β in PD patients 1,0396 (range 0,1540- 3,6432) vs 1 (control group).CONCLUSIONS: The expression of cytokines in tunica albuginea of PD patients compared to the control patients do not show anysignificative difference. Cytokines promoting inflammation areundetectable in PD patients and not seem to be involved in the pathogenesis of the disease. A higher level, althoug the difference wasnot statistically significative of the TGF- β, a profibrotic cytokine, wasdetected in PD patients representing a possible explain action of fibtotic tissue when the inflammation is off.
|Numero di pagine||1|
|Stato di pubblicazione||Published - 2012|
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