Cytokine serum profile in a group of Sicilian Nonagenarians

Letizia Scola, Calogero Caruso, Loredana Vaccarino, Gabriella Misiano, Marisa Palmeri, Giusi Irma Forte, Domenico Lio, Mariano Malaguarnera, Domenico Maugeri, Massimo Motta, Salvatore Milano

Risultato della ricerca: Article

13 Citazioni (Scopus)

Abstract

The aim of our study was to evaluate the possibility of using multiplex analysis of the cytokine profile as a marker for successful aging by comparing cytokine plasmatic levels of a group of Sicilian nonagenarians with those of young controls. We analyzed a panel of 17 cytokines, comprehensive of haematopoietic factors T helper 1 (Th1), Th2, inflammation regulatory cytokines, and chemokines. The assay was carried out using the Luminex system. Interleukin (IL)-6 levels (p = 0.01) were increased in nonagenarians, whereas no modifications of other proinflammatory cytokines and chemokines were observed. Interferon-gamma (IFN-gamma) and IL-2 levels are unmodified, suggesting a substantial maintenance of relevant T cell functions. In addition, a significant increase of IL-12 serum levels in nonagenarians versus young controls that might be related to the increase of natural killer (NK) cell functions characterizing aging processes was observed. The analysis of Th2 cytokines show an increase of IL-13 and a reduction of IL-4 levels mirroring the maintenance of some effector's mechanisms of the immunoresponse in advanced ages. Our results suggest that the multiplex analysis of cytokine levels might be useful in defining a successful aging profile.
Lingua originaleEnglish
pagine (da-a)82-90
Numero di pagine9
RivistaJOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
Volume33
Stato di pubblicazionePublished - 2012

Fingerprint

Cytokines
Serum
Aging of materials
Chemokines
Maintenance
T-cells
Interleukin-13
Interleukin-12
Interleukin-1
Natural Killer Cells
Interleukin-4
Interferon-gamma
Interleukin-2
Interleukin-6
Assays
Inflammation
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Medical Laboratory Technology

Cita questo

@article{9944b86ab1d0408dbed3203badccc7ff,
title = "Cytokine serum profile in a group of Sicilian Nonagenarians",
abstract = "The aim of our study was to evaluate the possibility of using multiplex analysis of the cytokine profile as a marker for successful aging by comparing cytokine plasmatic levels of a group of Sicilian nonagenarians with those of young controls. We analyzed a panel of 17 cytokines, comprehensive of haematopoietic factors T helper 1 (Th1), Th2, inflammation regulatory cytokines, and chemokines. The assay was carried out using the Luminex system. Interleukin (IL)-6 levels (p = 0.01) were increased in nonagenarians, whereas no modifications of other proinflammatory cytokines and chemokines were observed. Interferon-gamma (IFN-gamma) and IL-2 levels are unmodified, suggesting a substantial maintenance of relevant T cell functions. In addition, a significant increase of IL-12 serum levels in nonagenarians versus young controls that might be related to the increase of natural killer (NK) cell functions characterizing aging processes was observed. The analysis of Th2 cytokines show an increase of IL-13 and a reduction of IL-4 levels mirroring the maintenance of some effector's mechanisms of the immunoresponse in advanced ages. Our results suggest that the multiplex analysis of cytokine levels might be useful in defining a successful aging profile.",
author = "Letizia Scola and Calogero Caruso and Loredana Vaccarino and Gabriella Misiano and Marisa Palmeri and Forte, {Giusi Irma} and Domenico Lio and Mariano Malaguarnera and Domenico Maugeri and Massimo Motta and Salvatore Milano",
year = "2012",
language = "English",
volume = "33",
pages = "82--90",
journal = "JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY",
issn = "1532-1819",

}

TY - JOUR

T1 - Cytokine serum profile in a group of Sicilian Nonagenarians

AU - Scola, Letizia

AU - Caruso, Calogero

AU - Vaccarino, Loredana

AU - Misiano, Gabriella

AU - Palmeri, Marisa

AU - Forte, Giusi Irma

AU - Lio, Domenico

AU - Malaguarnera, Mariano

AU - Maugeri, Domenico

AU - Motta, Massimo

AU - Milano, Salvatore

PY - 2012

Y1 - 2012

N2 - The aim of our study was to evaluate the possibility of using multiplex analysis of the cytokine profile as a marker for successful aging by comparing cytokine plasmatic levels of a group of Sicilian nonagenarians with those of young controls. We analyzed a panel of 17 cytokines, comprehensive of haematopoietic factors T helper 1 (Th1), Th2, inflammation regulatory cytokines, and chemokines. The assay was carried out using the Luminex system. Interleukin (IL)-6 levels (p = 0.01) were increased in nonagenarians, whereas no modifications of other proinflammatory cytokines and chemokines were observed. Interferon-gamma (IFN-gamma) and IL-2 levels are unmodified, suggesting a substantial maintenance of relevant T cell functions. In addition, a significant increase of IL-12 serum levels in nonagenarians versus young controls that might be related to the increase of natural killer (NK) cell functions characterizing aging processes was observed. The analysis of Th2 cytokines show an increase of IL-13 and a reduction of IL-4 levels mirroring the maintenance of some effector's mechanisms of the immunoresponse in advanced ages. Our results suggest that the multiplex analysis of cytokine levels might be useful in defining a successful aging profile.

AB - The aim of our study was to evaluate the possibility of using multiplex analysis of the cytokine profile as a marker for successful aging by comparing cytokine plasmatic levels of a group of Sicilian nonagenarians with those of young controls. We analyzed a panel of 17 cytokines, comprehensive of haematopoietic factors T helper 1 (Th1), Th2, inflammation regulatory cytokines, and chemokines. The assay was carried out using the Luminex system. Interleukin (IL)-6 levels (p = 0.01) were increased in nonagenarians, whereas no modifications of other proinflammatory cytokines and chemokines were observed. Interferon-gamma (IFN-gamma) and IL-2 levels are unmodified, suggesting a substantial maintenance of relevant T cell functions. In addition, a significant increase of IL-12 serum levels in nonagenarians versus young controls that might be related to the increase of natural killer (NK) cell functions characterizing aging processes was observed. The analysis of Th2 cytokines show an increase of IL-13 and a reduction of IL-4 levels mirroring the maintenance of some effector's mechanisms of the immunoresponse in advanced ages. Our results suggest that the multiplex analysis of cytokine levels might be useful in defining a successful aging profile.

UR - http://hdl.handle.net/10447/73607

M3 - Article

VL - 33

SP - 82

EP - 90

JO - JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY

JF - JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY

SN - 1532-1819

ER -