Cytokine serum profile in a group of Sicilian Nonagenarians

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Abstract

The aim of our study was to evaluate the possibility of using multiplex analysis of the cytokine profile as a marker for successful aging by comparing cytokine plasmatic levels of a group of Sicilian nonagenarians with those of young controls. We analyzed a panel of 17 cytokines, comprehensive of haematopoietic factors T helper 1 (Th1), Th2, inflammation regulatory cytokines, and chemokines. The assay was carried out using the Luminex system. Interleukin (IL)-6 levels (p = 0.01) were increased in nonagenarians, whereas no modifications of other proinflammatory cytokines and chemokines were observed. Interferon-gamma (IFN-gamma) and IL-2 levels are unmodified, suggesting a substantial maintenance of relevant T cell functions. In addition, a significant increase of IL-12 serum levels in nonagenarians versus young controls that might be related to the increase of natural killer (NK) cell functions characterizing aging processes was observed. The analysis of Th2 cytokines show an increase of IL-13 and a reduction of IL-4 levels mirroring the maintenance of some effector's mechanisms of the immunoresponse in advanced ages. Our results suggest that the multiplex analysis of cytokine levels might be useful in defining a successful aging profile.
Lingua originaleEnglish
pagine (da-a)82-90
Numero di pagine9
RivistaJOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
Volume33
Stato di pubblicazionePublished - 2012

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Cytokines
Serum
Aging of materials
Chemokines
Maintenance
T-cells
Interleukin-13
Interleukin-12
Interleukin-1
Natural Killer Cells
Interleukin-4
Interferon-gamma
Interleukin-2
Interleukin-6
Assays
Inflammation
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Medical Laboratory Technology
  • Clinical Biochemistry
  • Immunology and Allergy

Cita questo

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title = "Cytokine serum profile in a group of Sicilian Nonagenarians",
abstract = "The aim of our study was to evaluate the possibility of using multiplex analysis of the cytokine profile as a marker for successful aging by comparing cytokine plasmatic levels of a group of Sicilian nonagenarians with those of young controls. We analyzed a panel of 17 cytokines, comprehensive of haematopoietic factors T helper 1 (Th1), Th2, inflammation regulatory cytokines, and chemokines. The assay was carried out using the Luminex system. Interleukin (IL)-6 levels (p = 0.01) were increased in nonagenarians, whereas no modifications of other proinflammatory cytokines and chemokines were observed. Interferon-gamma (IFN-gamma) and IL-2 levels are unmodified, suggesting a substantial maintenance of relevant T cell functions. In addition, a significant increase of IL-12 serum levels in nonagenarians versus young controls that might be related to the increase of natural killer (NK) cell functions characterizing aging processes was observed. The analysis of Th2 cytokines show an increase of IL-13 and a reduction of IL-4 levels mirroring the maintenance of some effector's mechanisms of the immunoresponse in advanced ages. Our results suggest that the multiplex analysis of cytokine levels might be useful in defining a successful aging profile.",
author = "Calogero Caruso and Gabriella Misiano and Salvatore Milano and Domenico Lio and Forte, {Giusi Irma} and Letizia Scola and Loredana Vaccarino and Marisa Palmeri and Domenico Maugeri and Massimo Motta and Mariano Malaguarnera",
year = "2012",
language = "English",
volume = "33",
pages = "82--90",
journal = "Journal of Immunoassay and Immunochemistry",
issn = "1532-1819",
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TY - JOUR

T1 - Cytokine serum profile in a group of Sicilian Nonagenarians

AU - Caruso, Calogero

AU - Misiano, Gabriella

AU - Milano, Salvatore

AU - Lio, Domenico

AU - Forte, Giusi Irma

AU - Scola, Letizia

AU - Vaccarino, Loredana

AU - Palmeri, Marisa

AU - Maugeri, Domenico

AU - Motta, Massimo

AU - Malaguarnera, Mariano

PY - 2012

Y1 - 2012

N2 - The aim of our study was to evaluate the possibility of using multiplex analysis of the cytokine profile as a marker for successful aging by comparing cytokine plasmatic levels of a group of Sicilian nonagenarians with those of young controls. We analyzed a panel of 17 cytokines, comprehensive of haematopoietic factors T helper 1 (Th1), Th2, inflammation regulatory cytokines, and chemokines. The assay was carried out using the Luminex system. Interleukin (IL)-6 levels (p = 0.01) were increased in nonagenarians, whereas no modifications of other proinflammatory cytokines and chemokines were observed. Interferon-gamma (IFN-gamma) and IL-2 levels are unmodified, suggesting a substantial maintenance of relevant T cell functions. In addition, a significant increase of IL-12 serum levels in nonagenarians versus young controls that might be related to the increase of natural killer (NK) cell functions characterizing aging processes was observed. The analysis of Th2 cytokines show an increase of IL-13 and a reduction of IL-4 levels mirroring the maintenance of some effector's mechanisms of the immunoresponse in advanced ages. Our results suggest that the multiplex analysis of cytokine levels might be useful in defining a successful aging profile.

AB - The aim of our study was to evaluate the possibility of using multiplex analysis of the cytokine profile as a marker for successful aging by comparing cytokine plasmatic levels of a group of Sicilian nonagenarians with those of young controls. We analyzed a panel of 17 cytokines, comprehensive of haematopoietic factors T helper 1 (Th1), Th2, inflammation regulatory cytokines, and chemokines. The assay was carried out using the Luminex system. Interleukin (IL)-6 levels (p = 0.01) were increased in nonagenarians, whereas no modifications of other proinflammatory cytokines and chemokines were observed. Interferon-gamma (IFN-gamma) and IL-2 levels are unmodified, suggesting a substantial maintenance of relevant T cell functions. In addition, a significant increase of IL-12 serum levels in nonagenarians versus young controls that might be related to the increase of natural killer (NK) cell functions characterizing aging processes was observed. The analysis of Th2 cytokines show an increase of IL-13 and a reduction of IL-4 levels mirroring the maintenance of some effector's mechanisms of the immunoresponse in advanced ages. Our results suggest that the multiplex analysis of cytokine levels might be useful in defining a successful aging profile.

UR - http://hdl.handle.net/10447/73607

M3 - Article

VL - 33

SP - 82

EP - 90

JO - Journal of Immunoassay and Immunochemistry

JF - Journal of Immunoassay and Immunochemistry

SN - 1532-1819

ER -