Cytokine profile of breast cell lines after different radiation doses

Domenico Lio, Giusi Irma Forte, Valentina Bravata', Luigi Minafra, Maria Carla Gilardi, Giuseppa Augello, Giorgio Russo, Valentina Bravatà, Francesco Paolo Cammarata, Giusi Irma Forte, Giuseppa Augello, Luigi Minafra

    Risultato della ricerca: Article

    8 Citazioni (Scopus)

    Abstract

    Purpose: Ionizing radiation (IR) treatment activates inflammatory processes causing the release of a great amount of molecules able to affect the cell survival. The aim of this study was to analyze the cytokine signature of conditioned medium produced by non-tumorigenic mammary epithelial cell line MCF10A, as well as MCF7 and MDA-MB-231 breast cancer cell lines, after single high doses of IR in order to understand their role in high radiation response. Materials and methods: We performed a cytokine profile of irradiated conditioned media of MCF10A, MCF7 and MDA-MB-231 cell lines treated with 9 or 23 Gy, by Luminex and ELISA analyses. Results: Overall, our results show that both 9 Gy and 23 Gy of IR induce the release within the first 72 h of cytokines and growth factors potentially able to influence the tumor outcome, with a dose-independent and cell-line dependent signature. Moreover, our results show that the cell-senescence phenomenon does not correlate with the amount of ‘senescence-associated secretory phenotype’ (SASP) molecules released in media. Thus, additional mechanisms are probably involved in this process. Conclusions: These data open the possibility to evaluate cytokine profile as useful marker in modulating the personalized radiotherapy in breast cancer care.
    Lingua originaleEnglish
    pagine (da-a)1217-1226
    Numero di pagine10
    RivistaInternational Journal of Radiation Biology
    Volume93
    Stato di pubblicazionePublished - 2017

    Fingerprint

    Breast
    Ionizing Radiation
    Radiation
    Cytokines
    Cell Line
    Conditioned Culture Medium
    Breast Neoplasms
    Radiation Dosage
    Cell Aging
    Cell Survival
    Intercellular Signaling Peptides and Proteins
    Radiotherapy
    Epithelial Cells
    Enzyme-Linked Immunosorbent Assay
    Phenotype
    Neoplasms
    Therapeutics

    All Science Journal Classification (ASJC) codes

    • Radiological and Ultrasound Technology
    • Radiology Nuclear Medicine and imaging

    Cita questo

    Cytokine profile of breast cell lines after different radiation doses. / Lio, Domenico; Forte, Giusi Irma; Bravata', Valentina; Minafra, Luigi; Gilardi, Maria Carla; Augello, Giuseppa; Russo, Giorgio; Bravatà, Valentina; Cammarata, Francesco Paolo; Forte, Giusi Irma; Augello, Giuseppa; Minafra, Luigi.

    In: International Journal of Radiation Biology, Vol. 93, 2017, pag. 1217-1226.

    Risultato della ricerca: Article

    Lio, D, Forte, GI, Bravata', V, Minafra, L, Gilardi, MC, Augello, G, Russo, G, Bravatà, V, Cammarata, FP, Forte, GI, Augello, G & Minafra, L 2017, 'Cytokine profile of breast cell lines after different radiation doses', International Journal of Radiation Biology, vol. 93, pagg. 1217-1226.
    Lio, Domenico ; Forte, Giusi Irma ; Bravata', Valentina ; Minafra, Luigi ; Gilardi, Maria Carla ; Augello, Giuseppa ; Russo, Giorgio ; Bravatà, Valentina ; Cammarata, Francesco Paolo ; Forte, Giusi Irma ; Augello, Giuseppa ; Minafra, Luigi. / Cytokine profile of breast cell lines after different radiation doses. In: International Journal of Radiation Biology. 2017 ; Vol. 93. pagg. 1217-1226.
    @article{11ca5076ea01457483aa6b9ee1f58e86,
    title = "Cytokine profile of breast cell lines after different radiation doses",
    abstract = "Purpose: Ionizing radiation (IR) treatment activates inflammatory processes causing the release of a great amount of molecules able to affect the cell survival. The aim of this study was to analyze the cytokine signature of conditioned medium produced by non-tumorigenic mammary epithelial cell line MCF10A, as well as MCF7 and MDA-MB-231 breast cancer cell lines, after single high doses of IR in order to understand their role in high radiation response. Materials and methods: We performed a cytokine profile of irradiated conditioned media of MCF10A, MCF7 and MDA-MB-231 cell lines treated with 9 or 23 Gy, by Luminex and ELISA analyses. Results: Overall, our results show that both 9 Gy and 23 Gy of IR induce the release within the first 72 h of cytokines and growth factors potentially able to influence the tumor outcome, with a dose-independent and cell-line dependent signature. Moreover, our results show that the cell-senescence phenomenon does not correlate with the amount of ‘senescence-associated secretory phenotype’ (SASP) molecules released in media. Thus, additional mechanisms are probably involved in this process. Conclusions: These data open the possibility to evaluate cytokine profile as useful marker in modulating the personalized radiotherapy in breast cancer care.",
    author = "Domenico Lio and Forte, {Giusi Irma} and Valentina Bravata' and Luigi Minafra and Gilardi, {Maria Carla} and Giuseppa Augello and Giorgio Russo and Valentina Bravat{\`a} and Cammarata, {Francesco Paolo} and Forte, {Giusi Irma} and Giuseppa Augello and Luigi Minafra",
    year = "2017",
    language = "English",
    volume = "93",
    pages = "1217--1226",
    journal = "International Journal of Radiation Biology",
    issn = "0955-3002",
    publisher = "Informa Healthcare",

    }

    TY - JOUR

    T1 - Cytokine profile of breast cell lines after different radiation doses

    AU - Lio, Domenico

    AU - Forte, Giusi Irma

    AU - Bravata', Valentina

    AU - Minafra, Luigi

    AU - Gilardi, Maria Carla

    AU - Augello, Giuseppa

    AU - Russo, Giorgio

    AU - Bravatà, Valentina

    AU - Cammarata, Francesco Paolo

    AU - Forte, Giusi Irma

    AU - Augello, Giuseppa

    AU - Minafra, Luigi

    PY - 2017

    Y1 - 2017

    N2 - Purpose: Ionizing radiation (IR) treatment activates inflammatory processes causing the release of a great amount of molecules able to affect the cell survival. The aim of this study was to analyze the cytokine signature of conditioned medium produced by non-tumorigenic mammary epithelial cell line MCF10A, as well as MCF7 and MDA-MB-231 breast cancer cell lines, after single high doses of IR in order to understand their role in high radiation response. Materials and methods: We performed a cytokine profile of irradiated conditioned media of MCF10A, MCF7 and MDA-MB-231 cell lines treated with 9 or 23 Gy, by Luminex and ELISA analyses. Results: Overall, our results show that both 9 Gy and 23 Gy of IR induce the release within the first 72 h of cytokines and growth factors potentially able to influence the tumor outcome, with a dose-independent and cell-line dependent signature. Moreover, our results show that the cell-senescence phenomenon does not correlate with the amount of ‘senescence-associated secretory phenotype’ (SASP) molecules released in media. Thus, additional mechanisms are probably involved in this process. Conclusions: These data open the possibility to evaluate cytokine profile as useful marker in modulating the personalized radiotherapy in breast cancer care.

    AB - Purpose: Ionizing radiation (IR) treatment activates inflammatory processes causing the release of a great amount of molecules able to affect the cell survival. The aim of this study was to analyze the cytokine signature of conditioned medium produced by non-tumorigenic mammary epithelial cell line MCF10A, as well as MCF7 and MDA-MB-231 breast cancer cell lines, after single high doses of IR in order to understand their role in high radiation response. Materials and methods: We performed a cytokine profile of irradiated conditioned media of MCF10A, MCF7 and MDA-MB-231 cell lines treated with 9 or 23 Gy, by Luminex and ELISA analyses. Results: Overall, our results show that both 9 Gy and 23 Gy of IR induce the release within the first 72 h of cytokines and growth factors potentially able to influence the tumor outcome, with a dose-independent and cell-line dependent signature. Moreover, our results show that the cell-senescence phenomenon does not correlate with the amount of ‘senescence-associated secretory phenotype’ (SASP) molecules released in media. Thus, additional mechanisms are probably involved in this process. Conclusions: These data open the possibility to evaluate cytokine profile as useful marker in modulating the personalized radiotherapy in breast cancer care.

    UR - http://hdl.handle.net/10447/326374

    UR - https://www.tandfonline.com/doi/abs/10.1080/09553002.2017.1362504?journalCode=irab20

    M3 - Article

    VL - 93

    SP - 1217

    EP - 1226

    JO - International Journal of Radiation Biology

    JF - International Journal of Radiation Biology

    SN - 0955-3002

    ER -