Cytokine profile of breast cell lines after different radiation doses

Domenico Lio, Valentina Bravata', Giusi Irma Forte, Luigi Minafra, Maria Carla Gilardi, Giuseppa Augello, Giorgio Russo, Valentina Bravatà, Francesco Paolo Cammarata, Giusi Irma Forte, Giuseppa Augello, Luigi Minafra

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    10 Citazioni (Scopus)

    Abstract

    Purpose: Ionizing radiation (IR) treatment activates inflammatory processes causing the release of a great amount of molecules able to affect the cell survival. The aim of this study was to analyze the cytokine signature of conditioned medium produced by non-tumorigenic mammary epithelial cell line MCF10A, as well as MCF7 and MDA-MB-231 breast cancer cell lines, after single high doses of IR in order to understand their role in high radiation response. Materials and methods: We performed a cytokine profile of irradiated conditioned media of MCF10A, MCF7 and MDA-MB-231 cell lines treated with 9 or 23 Gy, by Luminex and ELISA analyses. Results: Overall, our results show that both 9 Gy and 23 Gy of IR induce the release within the first 72 h of cytokines and growth factors potentially able to influence the tumor outcome, with a dose-independent and cell-line dependent signature. Moreover, our results show that the cell-senescence phenomenon does not correlate with the amount of ‘senescence-associated secretory phenotype’ (SASP) molecules released in media. Thus, additional mechanisms are probably involved in this process. Conclusions: These data open the possibility to evaluate cytokine profile as useful marker in modulating the personalized radiotherapy in breast cancer care.
    Lingua originaleEnglish
    pagine (da-a)1217-1226
    Numero di pagine10
    RivistaInternational Journal of Radiation Biology
    Volume93
    Stato di pubblicazionePublished - 2017

    All Science Journal Classification (ASJC) codes

    • Radiological and Ultrasound Technology
    • Radiology Nuclear Medicine and imaging

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    Lio, D., Bravata', V., Forte, G. I., Minafra, L., Gilardi, M. C., Augello, G., Russo, G., Bravatà, V., Cammarata, F. P., Forte, G. I., Augello, G., & Minafra, L. (2017). Cytokine profile of breast cell lines after different radiation doses. International Journal of Radiation Biology, 93, 1217-1226.