Cryopreservation of Limonium serotinum apical meristems from in vitro plantletsusing droplet-vitrification

Giovanni Iapichino, Giuseppe Barraco, Florent Engelmann, Isabelle Sylvestre, Giuseppe Barraco

Risultato della ricerca: Article

11 Citazioni (Scopus)

Abstract

In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreservedusing the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips waspossible only when samples were pretreated for 16 h in liquid medium with 0.3Msucrose, then for 5 h inliquid medium with 0.7M sucrose before performing the cryopreservation protocol. Optimal conditionsincluded treatment for 20 min in a loading solution containing 1.9M glycerol + 0.5M sucrose, treatmentwith vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solutionA9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapidcooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloadingsolution containing 1.2M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tipswas achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.
Lingua originaleEnglish
pagine (da-a)309-313
Numero di pagine5
RivistaDefault journal
Volume130
Stato di pubblicazionePublished - 2011

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Limonium
vitrification
apical meristems
cryopreservation
droplets
sucrose
glycerol
ethylene glycol
callus formation
dimethyl sulfoxide
regrowth
in vitro studies
sampling
shoots
liquids
genotype

All Science Journal Classification (ASJC) codes

  • Horticulture

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Cryopreservation of Limonium serotinum apical meristems from in vitro plantletsusing droplet-vitrification. / Iapichino, Giovanni; Barraco, Giuseppe; Engelmann, Florent; Sylvestre, Isabelle; Barraco, Giuseppe.

In: Default journal, Vol. 130, 2011, pag. 309-313.

Risultato della ricerca: Article

Iapichino, G, Barraco, G, Engelmann, F, Sylvestre, I & Barraco, G 2011, 'Cryopreservation of Limonium serotinum apical meristems from in vitro plantletsusing droplet-vitrification', Default journal, vol. 130, pagg. 309-313.
Iapichino, Giovanni ; Barraco, Giuseppe ; Engelmann, Florent ; Sylvestre, Isabelle ; Barraco, Giuseppe. / Cryopreservation of Limonium serotinum apical meristems from in vitro plantletsusing droplet-vitrification. In: Default journal. 2011 ; Vol. 130. pagg. 309-313.
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AU - Barraco, Giuseppe

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AB - In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreservedusing the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips waspossible only when samples were pretreated for 16 h in liquid medium with 0.3Msucrose, then for 5 h inliquid medium with 0.7M sucrose before performing the cryopreservation protocol. Optimal conditionsincluded treatment for 20 min in a loading solution containing 1.9M glycerol + 0.5M sucrose, treatmentwith vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solutionA9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapidcooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloadingsolution containing 1.2M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tipswas achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.

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