In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreservedusing the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips waspossible only when samples were pretreated for 16 h in liquid medium with 0.3Msucrose, then for 5 h inliquid medium with 0.7M sucrose before performing the cryopreservation protocol. Optimal conditionsincluded treatment for 20 min in a loading solution containing 1.9M glycerol + 0.5M sucrose, treatmentwith vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solutionA9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapidcooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloadingsolution containing 1.2M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tipswas achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.
|Numero di pagine||5|
|Stato di pubblicazione||Published - 2011|
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