Correlates of Human Herpesvirus-8 DNA detection among adults in Italy without Kaposi sarcoma

Francesco Vitale, Fabio Tramuto, Concetta Castilletti, Catia Valdarchi, Carmela Lauria, Pierluca Piselli, Anna Crispo, Paolo Ascierto, Michele Massimino, Anthony J. Alberg, Maria Rosaria De Marco, Mario Tamburini, Stefania Stella, Diego Serraino, Barbara Kroner, Denise Whitby, Mauizio Montella, Robert J. Biggar, Massimo Giuliani, Angelo Messina & 11 others Giovanni Rezza, Vickie Marshall, Carla Del Carpio, Sonia De Sicato, Caruso Laura, Paola Cordiali Fei, Charles S. Rabkin, Rosa Maria Corona, Lorenzo Gafa, James J. Goedert, Elizabeth E. Brown

Risultato della ricerca: Article

15 Citazioni (Scopus)

Abstract

Background: The presence of Human Herpesvirus-8 (HHV8) DNA is predictive of Kaposi sarcoma (KS) among patients with HIV-associated or iatrogenic immunosuppression. However, correlates of HHV8-DNA detection in the general population remain undefined. Methods: We assessed correlates of HHV8-DNA detection among Italian adults without KS who had antibodies against HHV8-latent nuclear antigen by immunofluorescence assay. HHV8-K6 DNA sequences were detected in peripheral blood mononuclear cells using TaqMan CR. Results: Of the 158 subjects 26 (16.5%) had detectable HHV8-DNA [median copies/million cells, 53; (13-2128)]. Adjusted for age, sex, and laboratory, HHV8-DNA was detected more frequently in participants with >7 total residents in the childhood home [OR = 3.7 (1.5-9.1)], >2 younger siblings [OR = 2.6 (1.1-6.5)], and current cardiovascular [OR = 3.6 (1.3-9.7)] or renal [OR = 3.1 (1.2-8.0)] disease. Excluding the participants using immune modulating drugs, HHV8-DNA was more frequent among those with low red blood cells (RBC) [<4.5 106/ μl; OR = 5.3 (1.7-16.2)], slightly elevated mean corpuscular volume [>92 μm3/red cell; OR = 2.8 (1.0-7.8)], and mild thrombocytopenia [<151 K/μl; OR = 5.6 1.9-16.3)]. Conclusions: Presence of HHV8-DNA in elderly Italians is associated with childhood crowding, low RBCs, and platelets, perhaps indicating roles for early infection and chronic inflammation. These risk factors are the first to be reported for non-immunosuppressed HHV8-seropositive adults.
Lingua originaleEnglish
pagine (da-a)1110-1117
Numero di pagine8
RivistaInternational Journal of Epidemiology
Volume34(5)
Stato di pubblicazionePublished - 2005

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Human Herpesvirus 8
Kaposi's Sarcoma
Italy
DNA
Crowding
Erythrocyte Indices
Thrombocytopenia
Immunosuppression
Fluorescent Antibody Technique
Siblings
Blood Cells
Blood Platelets
Erythrocytes
HIV
Inflammation
Kidney

All Science Journal Classification (ASJC) codes

  • Epidemiology

Cita questo

Vitale, F., Tramuto, F., Castilletti, C., Valdarchi, C., Lauria, C., Piselli, P., ... Brown, E. E. (2005). Correlates of Human Herpesvirus-8 DNA detection among adults in Italy without Kaposi sarcoma. International Journal of Epidemiology, 34(5), 1110-1117.

Correlates of Human Herpesvirus-8 DNA detection among adults in Italy without Kaposi sarcoma. / Vitale, Francesco; Tramuto, Fabio; Castilletti, Concetta; Valdarchi, Catia; Lauria, Carmela; Piselli, Pierluca; Crispo, Anna; Ascierto, Paolo; Massimino, Michele; Alberg, Anthony J.; De Marco, Maria Rosaria; Tamburini, Mario; Stella, Stefania; Serraino, Diego; Kroner, Barbara; Whitby, Denise; Montella, Mauizio; Biggar, Robert J.; Giuliani, Massimo; Messina, Angelo; Rezza, Giovanni; Marshall, Vickie; Del Carpio, Carla; De Sicato, Sonia; Laura, Caruso; Fei, Paola Cordiali; Rabkin, Charles S.; Corona, Rosa Maria; Gafa, Lorenzo; Goedert, James J.; Brown, Elizabeth E.

In: International Journal of Epidemiology, Vol. 34(5), 2005, pag. 1110-1117.

Risultato della ricerca: Article

Vitale, F, Tramuto, F, Castilletti, C, Valdarchi, C, Lauria, C, Piselli, P, Crispo, A, Ascierto, P, Massimino, M, Alberg, AJ, De Marco, MR, Tamburini, M, Stella, S, Serraino, D, Kroner, B, Whitby, D, Montella, M, Biggar, RJ, Giuliani, M, Messina, A, Rezza, G, Marshall, V, Del Carpio, C, De Sicato, S, Laura, C, Fei, PC, Rabkin, CS, Corona, RM, Gafa, L, Goedert, JJ & Brown, EE 2005, 'Correlates of Human Herpesvirus-8 DNA detection among adults in Italy without Kaposi sarcoma', International Journal of Epidemiology, vol. 34(5), pagg. 1110-1117.
Vitale, Francesco ; Tramuto, Fabio ; Castilletti, Concetta ; Valdarchi, Catia ; Lauria, Carmela ; Piselli, Pierluca ; Crispo, Anna ; Ascierto, Paolo ; Massimino, Michele ; Alberg, Anthony J. ; De Marco, Maria Rosaria ; Tamburini, Mario ; Stella, Stefania ; Serraino, Diego ; Kroner, Barbara ; Whitby, Denise ; Montella, Mauizio ; Biggar, Robert J. ; Giuliani, Massimo ; Messina, Angelo ; Rezza, Giovanni ; Marshall, Vickie ; Del Carpio, Carla ; De Sicato, Sonia ; Laura, Caruso ; Fei, Paola Cordiali ; Rabkin, Charles S. ; Corona, Rosa Maria ; Gafa, Lorenzo ; Goedert, James J. ; Brown, Elizabeth E. / Correlates of Human Herpesvirus-8 DNA detection among adults in Italy without Kaposi sarcoma. In: International Journal of Epidemiology. 2005 ; Vol. 34(5). pagg. 1110-1117.
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title = "Correlates of Human Herpesvirus-8 DNA detection among adults in Italy without Kaposi sarcoma",
abstract = "Background: The presence of Human Herpesvirus-8 (HHV8) DNA is predictive of Kaposi sarcoma (KS) among patients with HIV-associated or iatrogenic immunosuppression. However, correlates of HHV8-DNA detection in the general population remain undefined. Methods: We assessed correlates of HHV8-DNA detection among Italian adults without KS who had antibodies against HHV8-latent nuclear antigen by immunofluorescence assay. HHV8-K6 DNA sequences were detected in peripheral blood mononuclear cells using TaqMan CR. Results: Of the 158 subjects 26 (16.5{\%}) had detectable HHV8-DNA [median copies/million cells, 53; (13-2128)]. Adjusted for age, sex, and laboratory, HHV8-DNA was detected more frequently in participants with >7 total residents in the childhood home [OR = 3.7 (1.5-9.1)], >2 younger siblings [OR = 2.6 (1.1-6.5)], and current cardiovascular [OR = 3.6 (1.3-9.7)] or renal [OR = 3.1 (1.2-8.0)] disease. Excluding the participants using immune modulating drugs, HHV8-DNA was more frequent among those with low red blood cells (RBC) [<4.5 106/ μl; OR = 5.3 (1.7-16.2)], slightly elevated mean corpuscular volume [>92 μm3/red cell; OR = 2.8 (1.0-7.8)], and mild thrombocytopenia [<151 K/μl; OR = 5.6 1.9-16.3)]. Conclusions: Presence of HHV8-DNA in elderly Italians is associated with childhood crowding, low RBCs, and platelets, perhaps indicating roles for early infection and chronic inflammation. These risk factors are the first to be reported for non-immunosuppressed HHV8-seropositive adults.",
keywords = "HHV8; Kaposi sarcoma herpesvirus; KSHV; viral DNA; viral load",
author = "Francesco Vitale and Fabio Tramuto and Concetta Castilletti and Catia Valdarchi and Carmela Lauria and Pierluca Piselli and Anna Crispo and Paolo Ascierto and Michele Massimino and Alberg, {Anthony J.} and {De Marco}, {Maria Rosaria} and Mario Tamburini and Stefania Stella and Diego Serraino and Barbara Kroner and Denise Whitby and Mauizio Montella and Biggar, {Robert J.} and Massimo Giuliani and Angelo Messina and Giovanni Rezza and Vickie Marshall and {Del Carpio}, Carla and {De Sicato}, Sonia and Caruso Laura and Fei, {Paola Cordiali} and Rabkin, {Charles S.} and Corona, {Rosa Maria} and Lorenzo Gafa and Goedert, {James J.} and Brown, {Elizabeth E.}",
year = "2005",
language = "English",
volume = "34(5)",
pages = "1110--1117",
journal = "International Journal of Epidemiology",
issn = "0300-5771",
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}

TY - JOUR

T1 - Correlates of Human Herpesvirus-8 DNA detection among adults in Italy without Kaposi sarcoma

AU - Vitale, Francesco

AU - Tramuto, Fabio

AU - Castilletti, Concetta

AU - Valdarchi, Catia

AU - Lauria, Carmela

AU - Piselli, Pierluca

AU - Crispo, Anna

AU - Ascierto, Paolo

AU - Massimino, Michele

AU - Alberg, Anthony J.

AU - De Marco, Maria Rosaria

AU - Tamburini, Mario

AU - Stella, Stefania

AU - Serraino, Diego

AU - Kroner, Barbara

AU - Whitby, Denise

AU - Montella, Mauizio

AU - Biggar, Robert J.

AU - Giuliani, Massimo

AU - Messina, Angelo

AU - Rezza, Giovanni

AU - Marshall, Vickie

AU - Del Carpio, Carla

AU - De Sicato, Sonia

AU - Laura, Caruso

AU - Fei, Paola Cordiali

AU - Rabkin, Charles S.

AU - Corona, Rosa Maria

AU - Gafa, Lorenzo

AU - Goedert, James J.

AU - Brown, Elizabeth E.

PY - 2005

Y1 - 2005

N2 - Background: The presence of Human Herpesvirus-8 (HHV8) DNA is predictive of Kaposi sarcoma (KS) among patients with HIV-associated or iatrogenic immunosuppression. However, correlates of HHV8-DNA detection in the general population remain undefined. Methods: We assessed correlates of HHV8-DNA detection among Italian adults without KS who had antibodies against HHV8-latent nuclear antigen by immunofluorescence assay. HHV8-K6 DNA sequences were detected in peripheral blood mononuclear cells using TaqMan CR. Results: Of the 158 subjects 26 (16.5%) had detectable HHV8-DNA [median copies/million cells, 53; (13-2128)]. Adjusted for age, sex, and laboratory, HHV8-DNA was detected more frequently in participants with >7 total residents in the childhood home [OR = 3.7 (1.5-9.1)], >2 younger siblings [OR = 2.6 (1.1-6.5)], and current cardiovascular [OR = 3.6 (1.3-9.7)] or renal [OR = 3.1 (1.2-8.0)] disease. Excluding the participants using immune modulating drugs, HHV8-DNA was more frequent among those with low red blood cells (RBC) [<4.5 106/ μl; OR = 5.3 (1.7-16.2)], slightly elevated mean corpuscular volume [>92 μm3/red cell; OR = 2.8 (1.0-7.8)], and mild thrombocytopenia [<151 K/μl; OR = 5.6 1.9-16.3)]. Conclusions: Presence of HHV8-DNA in elderly Italians is associated with childhood crowding, low RBCs, and platelets, perhaps indicating roles for early infection and chronic inflammation. These risk factors are the first to be reported for non-immunosuppressed HHV8-seropositive adults.

AB - Background: The presence of Human Herpesvirus-8 (HHV8) DNA is predictive of Kaposi sarcoma (KS) among patients with HIV-associated or iatrogenic immunosuppression. However, correlates of HHV8-DNA detection in the general population remain undefined. Methods: We assessed correlates of HHV8-DNA detection among Italian adults without KS who had antibodies against HHV8-latent nuclear antigen by immunofluorescence assay. HHV8-K6 DNA sequences were detected in peripheral blood mononuclear cells using TaqMan CR. Results: Of the 158 subjects 26 (16.5%) had detectable HHV8-DNA [median copies/million cells, 53; (13-2128)]. Adjusted for age, sex, and laboratory, HHV8-DNA was detected more frequently in participants with >7 total residents in the childhood home [OR = 3.7 (1.5-9.1)], >2 younger siblings [OR = 2.6 (1.1-6.5)], and current cardiovascular [OR = 3.6 (1.3-9.7)] or renal [OR = 3.1 (1.2-8.0)] disease. Excluding the participants using immune modulating drugs, HHV8-DNA was more frequent among those with low red blood cells (RBC) [<4.5 106/ μl; OR = 5.3 (1.7-16.2)], slightly elevated mean corpuscular volume [>92 μm3/red cell; OR = 2.8 (1.0-7.8)], and mild thrombocytopenia [<151 K/μl; OR = 5.6 1.9-16.3)]. Conclusions: Presence of HHV8-DNA in elderly Italians is associated with childhood crowding, low RBCs, and platelets, perhaps indicating roles for early infection and chronic inflammation. These risk factors are the first to be reported for non-immunosuppressed HHV8-seropositive adults.

KW - HHV8; Kaposi sarcoma herpesvirus; KSHV; viral DNA; viral load

UR - http://hdl.handle.net/10447/8438

M3 - Article

VL - 34(5)

SP - 1110

EP - 1117

JO - International Journal of Epidemiology

JF - International Journal of Epidemiology

SN - 0300-5771

ER -