Construction and evaluation of sponge scaffolds from hyaluronic acid derivatives for potential cartilage regeneration

Giovanna Pitarresi, Gaetano Giammona, Fabio Salvatore Palumbo, Mauro Di Stefano, Alessandro Gulino, Calogero Fiorica, Alessandro Gulino, Francesca Abramo, Alessandro Gulino, Mario Giorgi, Giovanna Pitarresi, Francesca Abramo, Gaetano Giammona

Risultato della ricerca: Articlepeer review

12 Citazioni (Scopus)


A two or one pot synthesis has been used for the reaction of hyaluronic acid (HA) with octadecylamine (C-18) and hydrazine (Hy). In both cases, the chemical derivatization involved primary hydroxyl groups of hyaluronic acid and not its carboxyl groups, whose presence is important for receptor interaction. In this way, Hy-HA-C-18 derivatives have been obtained with appropriate hydrophobic and hydrophilic character. Their ability to form homogeneous physical hydrogels has been evaluated as well as the possibility to obtain porous sponges through salt leaching technology. Sponges showing the highest porosity, potentially compatible with cell entrapment, have been characterized with regard to their physicochemical and biological properties. Swelling ability under simulated physiological conditions and stability in the absence or in the presence of hyaluronidase have been investigated. Bovine chondrocytes were viable in Hy-HA-C-18 sponges as determined with MTS assay and were able to produce collagen and glycosaminoglycans, as assessed by using Masson's trichrome and Alcian blue, respectively. Finally, in vivo degradation of Hy-HA-C-18 sponges has been confirmed after subcutaneous implantation in mice until 6 weeks
Lingua originaleEnglish
pagine (da-a)3243-3253
Numero di pagine11
RivistaJournal of Materials Chemistry
Stato di pubblicazionePublished - 2014

All Science Journal Classification (ASJC) codes

  • ???subjectarea.asjc.1600.1600???
  • ???subjectarea.asjc.2200.2204???
  • ???subjectarea.asjc.2500.2500???


Entra nei temi di ricerca di 'Construction and evaluation of sponge scaffolds from hyaluronic acid derivatives for potential cartilage regeneration'. Insieme formano una fingerprint unica.

Cita questo