Constitutive hsp70 is essential to mitosis during early cleavage of Paracentrotus lividus embryos: The blockage of constitutive hsp70 impairs mitosis

Fabiana Geraci, Gabriella Sconzo, Franco Palla, Giovanni Spinelli, Franco Palla, Cecilia Agueli, Domenico Cascino, Giovanni Giudice

Risultato della ricerca: Articlepeer review

26 Citazioni (Scopus)

Abstract

Localization of constitutive hsp70 in eggs and early embryos of sea urchin Paracentrotus lividus is shown by means of in situ immunostaining. An accumulation of this protein is shown in the mitotic structures tasters, spindles and centrosomes). Microinjection of anti-hsp70 antibodies into eggs causes impairment of formation of mitotic structures and of cell division. This impairment goes from a complete mitotic block, to irregular mitotic apparatus formation with irregular cleavage, depending upon the antibody concentration. The localization of hsp70 after antibody microinjection is also described. Blockage of mitotic apparatus formation by nocodazole also blocks the concentration of hsp70 molecules observed in nontreated eggs. That the constitutive hsp70 plays a role in sea urchin mitosis is indicated.
Lingua originaleEnglish
pagine (da-a)143-149
Numero di pagine7
RivistaBiochemical and Biophysical Research Communications
Volume260
Stato di pubblicazionePublished - 1999

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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