Congenital reduction in number of nephrons accelerates renal damage, and limits proximal tubular growth response to release of neonatal partial ureteral obstruction

Risultato della ricerca: Other

Abstract

Aim: to analyze the evolution of the response to persistent partial ureteral obstruction (PUO) andobstruction/release (PUO/Rel) in mice with 50% reduction in the number of nephrons from 21days of age (time of weaning) to 42 days of age (adulthood).Methods: C57/BL6 wild-type (WT) and mice with oligosyndactylism and 50% reduction of nephronnumber (Os/+), were subjected to sham operation or PUO in the first 2 days of life. Additionalmice underwent release of obstruction at 7 days (PUO-Rel). The study was performed in kidneysharvested at 21 and 42 days. Using histomorphometry, glomerular area (GA) was determined byPAS staining, and fractional proximal tubular (PT) mass and intact glomerulotubular junctions (GTJ)were measured in Lotus tetragonolobus lectin-stained kidneys.Results:Mean per group (n = 6-14 mice per group). P<0.05 †vs WT, ‡vs sham, *vs 21 dBody weight doubled and kidney weight increased ~50% from 21 to 42 days of age in all mice. At42 days, kidney weight was lower in Os/+ than WT kidneys, regardless of obstruction. Glomerulararea increased with age in all groups except in WT PUO/Rel. In contrast to WT, glomerular areawas reduced by PUO in Os/+ mice at both 21 and 42 days. Fractional PT mass increased with ageof sham-operated mice, but this was prevented by PUO in both strains. Release of obstructionprevented the decrease of PT mass in 42 day-old WT, but not in Os/+ mice. Fraction of intact GTJincreased with age in sham kidneys in both strains, but did not increase in obstructed kidneysregardless of release. Intact GTJ % was decreased by PUO in both strains regardless of age; thiswas prevented by release of obstruction.Conclusions: PUO impaired glomerular growth in Os/+ but not WT mice, and prevented GTJmaturation and PT growth in both strains. Release of obstruction had no effect on glomerulararea, allowed GTJ maturation in both strains, and permitted PT growth in WT but not Os/+ mice.Because preterm and intrauterine growth-restricted infants are born with reduced nephronnumber, these patients may be at greater risk for renal injury due to urinary tract obstruction
Lingua originaleEnglish
Numero di pagine1
Stato di pubblicazionePublished - 2012

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