Confocal microscopy study of the distribution, content and activity of mitochondria during Paracentrotus lividus development

Maria Carmela Roccheri, Filippo Spagnolo, Carlo Maria Di Liegro, Anna Maria Rinaldi, Giovanni Morici, Maria Agnello

Risultato della ricerca: Articlepeer review

18 Citazioni (Scopus)

Abstract

In the present paper we applied confocal microscopy and fluorescence technologies for studying the distribution and the oxidative activity of sea urchin (Paracentrotus lividus) mitochondria during development, by in vivo incubating eggs and embryos with cell-permeant MitoTracker probes. We calculated, by a mathematical model, the intensity values, the variations of intensity, and the variation index of incorporated fluorochromes. Data demonstrate that mitochondrial mass does not change during development, whereas mitochondrial respiration increases. In addition, starting from 16 blastomeres stage, some regions of the embryo contain organelles more active in oxygen consumption.
Lingua originaleEnglish
pagine (da-a)165-173
Numero di pagine9
RivistaJournal of Microscopy
Volume228
Stato di pubblicazionePublished - 2007

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

Fingerprint Entra nei temi di ricerca di 'Confocal microscopy study of the distribution, content and activity of mitochondria during Paracentrotus lividus development'. Insieme formano una fingerprint unica.

Cita questo