Complete sequence, genotyping and comparative analysis of Pepino mosaic virus discovered isolates from Italy

Salvatore Davino, Mario Davino, Tiberini, Davino, Tomassoli

Risultato della ricerca: Article

4 Citazioni (Scopus)

Abstract

Pepino mosaic virus (PepMV) causes economic damageto the tomato industry worldwide. In Italy, the virusis endemic in Sicily, an important tomato-production region,and a new outbreak has been recorded in Sardiniaafter the first PepMV appearance in 2001. Italian PepMVisolates from these two geographically distant regionswere molecularly characterized and the complete nucleotidesequence of four isolates was determined bywalking primer strategy. The complete nucleotide anddeduced amino acid sequences were analyzed and comparedwith published PepMV sequences. Italian isolatesbelong to the PepMV-Ch2 strain and had 97.2% to98.5% identity with those of the reference PepMV-Ch2deposited in GenBank. The two Sicilian isolates wereslightly more variable in the RdRp and TGB1 genes.This study provides new insights into the occurrenceand variability of PepMV isolates in Italy.
Lingua originaleEnglish
pagine (da-a)437-442
Numero di pagine6
RivistaJournal of Plant Pathology
Volume93
Stato di pubblicazionePublished - 2011

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Pepino mosaic virus
genotyping
Italy
tomatoes
Sicily
amino acid sequences
nucleotides
industry
economics

All Science Journal Classification (ASJC) codes

  • Plant Science

Cita questo

Complete sequence, genotyping and comparative analysis of Pepino mosaic virus discovered isolates from Italy. / Davino, Salvatore; Davino, Mario; Tiberini; Davino; Tomassoli.

In: Journal of Plant Pathology, Vol. 93, 2011, pag. 437-442.

Risultato della ricerca: Article

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abstract = "Pepino mosaic virus (PepMV) causes economic damageto the tomato industry worldwide. In Italy, the virusis endemic in Sicily, an important tomato-production region,and a new outbreak has been recorded in Sardiniaafter the first PepMV appearance in 2001. Italian PepMVisolates from these two geographically distant regionswere molecularly characterized and the complete nucleotidesequence of four isolates was determined bywalking primer strategy. The complete nucleotide anddeduced amino acid sequences were analyzed and comparedwith published PepMV sequences. Italian isolatesbelong to the PepMV-Ch2 strain and had 97.2{\%} to98.5{\%} identity with those of the reference PepMV-Ch2deposited in GenBank. The two Sicilian isolates wereslightly more variable in the RdRp and TGB1 genes.This study provides new insights into the occurrenceand variability of PepMV isolates in Italy.",
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AU - Davino, null

AU - Tomassoli, null

PY - 2011

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AB - Pepino mosaic virus (PepMV) causes economic damageto the tomato industry worldwide. In Italy, the virusis endemic in Sicily, an important tomato-production region,and a new outbreak has been recorded in Sardiniaafter the first PepMV appearance in 2001. Italian PepMVisolates from these two geographically distant regionswere molecularly characterized and the complete nucleotidesequence of four isolates was determined bywalking primer strategy. The complete nucleotide anddeduced amino acid sequences were analyzed and comparedwith published PepMV sequences. Italian isolatesbelong to the PepMV-Ch2 strain and had 97.2% to98.5% identity with those of the reference PepMV-Ch2deposited in GenBank. The two Sicilian isolates wereslightly more variable in the RdRp and TGB1 genes.This study provides new insights into the occurrenceand variability of PepMV isolates in Italy.

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