Complete decontamination and regeneration of DNA purification silica colum

Risultato della ricerca: Article

3 Citazioni (Scopus)

Abstract

Silica columns are among the most used DNA purification systems, allowing a good yield of high-quality nucleic acids without organic extractions. Silica column regeneration protocols reported up to now toremove DNA traces are time-consuming, and their effectiveness on genomic DNA has not been demonstrated. Here we report a very rapid regeneration procedure that ensures no DNA carryover, independentof its size, without impairing column efficiency. The method takes advantage of the improved DNA removal by low concentrations of TritonX-100.
Lingua originaleEnglish
pagine (da-a)182-183
Numero di pagine2
RivistaAnalytical Biochemistry
Volume385
Stato di pubblicazionePublished - 2009

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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