Comparative Study of Human and Automated Screening for Antinuclear Antibodies by Immunofluorescence on HEp-2 Cells

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Abstract

Background: Several automated systems had been developed in order to reduce inter-observer variability inindirect immunofluorescence (IIF) interpretation. We aimed to evaluate the performance of a processing system inantinuclear antibodies (ANA) screening on HEp-2 cells.Patients and Methods: This study included 64 ANA-positive sera and 107 ANA-negative sera that underwent IIF on twocommercial kits of HEp-2 cells (BioSystems® and Euroimmun®). IIF results were compared with a novel automatedinterpretation system, the “CyclopusCADImmuno®” (CAD).Results: All ANA-positive sera images were recognized as positive by CAD (sensitivity = 100%), while 17 (15.9%) of theANA-negative sera images were interpreted as positive (specificity = 84.1%), =0.799 (SD=0.045). Comparison of IIFpattern determination between human and CAD system revealed on HEp-2 (BioSystems®), a complete concordance in6 (9.37%) sera, a partial concordance (sharing of at least 1 pattern) in 42 (65.6%) cases and in 16 (25%) sera thepattern interpretation was discordant. Similarly, on HEp-2 (Euroimmun®) the concordance in pattern interpretation wastotal in 5 (7.8%) sera, partial in 39 (60.9%) and absent in 20 (31.25%). For both tested HEp-2 cells kits agreement wasenhanced for the most common patterns, homogenous, fine speckled and coarse speckled. While there was an issue inidentification of nucleolar, dots and nuclear membranous patterns by CAD.Conclusion: Assessment of ANA by IIF on HEp-2 cells using the automated interpretation system, the“CyclopusCADImmuno®” is a reliable method for positive/negative differentiation. Continuous integration of IIF imageswould improve the pattern identification by the CAD.
Lingua originaleEnglish
pagine (da-a)270-276
Numero di pagine7
RivistaINTERNATIONAL JOURNAL OF STATISTICS IN MEDICAL RESEARCH
Volume4
Stato di pubblicazionePublished - 2015

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