The extracellular matrix (ECM) underlying epithelial tissues isinvolved in the maintenance of cell polarity and homeostasis.ECM is a dynamic structure under the regulated remodeling ofits components. The major enzymes responsible of matrix degradationare the matrix metalloproteinases (MMPs), a well knownfamily of zinc-dependent endopeptidases. Much attention hasbeen focused on MMP-2 and MMP-9 because of their ability todegrade type IV collagen, a major constituent of basementmembranes.A deregulated proteolysis of ECM molecules may cause thealteration of cell polarity and may contribute to the disruptionof cell–cell and cell–ECM adhesions, promoting cancer progression.These alterations are responsible for a poor prognosis, anda positive correlation between the increase of MMPs and thedegree of malignancy has also been observed FOR many tumorhistotypes. To approach these issues on in vitro models, we performeda comparative study, between a couple of tumoral andnon-tumoral mammary cell lines and a couple of thyroid celllines derived respectively from a benign and malignant cancer.This experimental approach, based on scanning electronmicroscopy, on proteomic analysis and on gelatin zimography,highlighted a similar profiling of the two differential couples ofcell lines: that is between malignant and non-malignant cellsrespectively, regardless of their histological origin.In particular, it was observed that the cell lines derived fromaggressive cancers, when compared with their non-malignantcounterpart, showed an increased secretion of MMPs, a cellshape highly pleomorphic and a higher expression of proteinclusters potentially associated with invasion and metastasis. Theanalysis of the interactions between the expression of MMPsand of selected proteomic clusters have offered important indicationon the complex network existing between neoplastic cellsand their environment.The work was co-funded by the Italian 5x1000 to COBS.
|Numero di pagine||1|
|Stato di pubblicazione||Published - 2015|