Combining inulin multifunctional polycation and magnetic nanoparticles: redox-responsive siRNA-loaded systems for magnetofection

Risultato della ricerca: Article

Abstract

Superparamagnetic Iron Oxide Nanoparticles (SPIONs) are recognized as one of the most promising agents for theranostic applications. Among methods designed for siRNA delivery, magnetofection, that is, nucleic acid cell uptake under the influence of a magnetic field acting on magnetic nucleic acid vectors, is emerging as a unique approach to combining advantages such as strong improvement of the kinetics of the delivery process and the possibility of localizing nucleic acid delivery to an area where the magnetic field is applied. This paper reports on the preparation of siRNA loaded magnetoplexes—named ICD@SS@SPIONs/siRNA—by controlled crosslinking, in the presence of SPIONs, of the polycation INU-C-DETA, synthesized starting from the polysaccharide inulin by grafting diethylenetriamine and cystamine molecules. The obtained ICD@SS@SPIONs/siRNA have suitable chemical-physical characteristics to be employed for iv administration and are also able to release siRNA in a redox-triggered manner thanks to intracellularglutathione (GSH) mediated reduction of disulphide bridges formed during the crosslinking process.Moreover, ICD@SS@SPIONs/siRNA are able to produce magnetic targeting in vitro on breast cancer cells, without appreciable cyto- and hemo-toxic eects, in a wide range of concentrations. Finally, protein binding to nanoparticles revealed that obtained systems are potentially longer circulating and applicable as a smart multifunctional agents for cancer therapy.
Lingua originaleEnglish
pagine (da-a)889-
Numero di pagine22
RivistaPolymers
Volume11
Stato di pubblicazionePublished - 2019

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Inulin
Small Interfering RNA
Iron oxides
Nanoparticles
Nucleic acids
Nucleic Acids
Crosslinking
Cystamine
DEET
Magnetic fields
Poisons
Polysaccharides
Disulfides
polycations
Oxidation-Reduction
Cells
ferric oxide
Molecules
Kinetics

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Polymers and Plastics

Cita questo

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title = "Combining inulin multifunctional polycation and magnetic nanoparticles: redox-responsive siRNA-loaded systems for magnetofection",
abstract = "Superparamagnetic Iron Oxide Nanoparticles (SPIONs) are recognized as one of the most promising agents for theranostic applications. Among methods designed for siRNA delivery, magnetofection, that is, nucleic acid cell uptake under the influence of a magnetic field acting on magnetic nucleic acid vectors, is emerging as a unique approach to combining advantages such as strong improvement of the kinetics of the delivery process and the possibility of localizing nucleic acid delivery to an area where the magnetic field is applied. This paper reports on the preparation of siRNA loaded magnetoplexes—named ICD@SS@SPIONs/siRNA—by controlled crosslinking, in the presence of SPIONs, of the polycation INU-C-DETA, synthesized starting from the polysaccharide inulin by grafting diethylenetriamine and cystamine molecules. The obtained ICD@SS@SPIONs/siRNA have suitable chemical-physical characteristics to be employed for iv administration and are also able to release siRNA in a redox-triggered manner thanks to intracellularglutathione (GSH) mediated reduction of disulphide bridges formed during the crosslinking process.Moreover, ICD@SS@SPIONs/siRNA are able to produce magnetic targeting in vitro on breast cancer cells, without appreciable cyto- and hemo-toxic eects, in a wide range of concentrations. Finally, protein binding to nanoparticles revealed that obtained systems are potentially longer circulating and applicable as a smart multifunctional agents for cancer therapy.",
author = "Gennara Cavallaro and Gaetano Giammona and Carla Sardo and Craparo, {Emanuela Fabiola} and Barbara Porsio",
year = "2019",
language = "English",
volume = "11",
pages = "889--",
journal = "Polymers",
issn = "2073-4360",
publisher = "MDPI AG",

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TY - JOUR

T1 - Combining inulin multifunctional polycation and magnetic nanoparticles: redox-responsive siRNA-loaded systems for magnetofection

AU - Cavallaro, Gennara

AU - Giammona, Gaetano

AU - Sardo, Carla

AU - Craparo, Emanuela Fabiola

AU - Porsio, Barbara

PY - 2019

Y1 - 2019

N2 - Superparamagnetic Iron Oxide Nanoparticles (SPIONs) are recognized as one of the most promising agents for theranostic applications. Among methods designed for siRNA delivery, magnetofection, that is, nucleic acid cell uptake under the influence of a magnetic field acting on magnetic nucleic acid vectors, is emerging as a unique approach to combining advantages such as strong improvement of the kinetics of the delivery process and the possibility of localizing nucleic acid delivery to an area where the magnetic field is applied. This paper reports on the preparation of siRNA loaded magnetoplexes—named ICD@SS@SPIONs/siRNA—by controlled crosslinking, in the presence of SPIONs, of the polycation INU-C-DETA, synthesized starting from the polysaccharide inulin by grafting diethylenetriamine and cystamine molecules. The obtained ICD@SS@SPIONs/siRNA have suitable chemical-physical characteristics to be employed for iv administration and are also able to release siRNA in a redox-triggered manner thanks to intracellularglutathione (GSH) mediated reduction of disulphide bridges formed during the crosslinking process.Moreover, ICD@SS@SPIONs/siRNA are able to produce magnetic targeting in vitro on breast cancer cells, without appreciable cyto- and hemo-toxic eects, in a wide range of concentrations. Finally, protein binding to nanoparticles revealed that obtained systems are potentially longer circulating and applicable as a smart multifunctional agents for cancer therapy.

AB - Superparamagnetic Iron Oxide Nanoparticles (SPIONs) are recognized as one of the most promising agents for theranostic applications. Among methods designed for siRNA delivery, magnetofection, that is, nucleic acid cell uptake under the influence of a magnetic field acting on magnetic nucleic acid vectors, is emerging as a unique approach to combining advantages such as strong improvement of the kinetics of the delivery process and the possibility of localizing nucleic acid delivery to an area where the magnetic field is applied. This paper reports on the preparation of siRNA loaded magnetoplexes—named ICD@SS@SPIONs/siRNA—by controlled crosslinking, in the presence of SPIONs, of the polycation INU-C-DETA, synthesized starting from the polysaccharide inulin by grafting diethylenetriamine and cystamine molecules. The obtained ICD@SS@SPIONs/siRNA have suitable chemical-physical characteristics to be employed for iv administration and are also able to release siRNA in a redox-triggered manner thanks to intracellularglutathione (GSH) mediated reduction of disulphide bridges formed during the crosslinking process.Moreover, ICD@SS@SPIONs/siRNA are able to produce magnetic targeting in vitro on breast cancer cells, without appreciable cyto- and hemo-toxic eects, in a wide range of concentrations. Finally, protein binding to nanoparticles revealed that obtained systems are potentially longer circulating and applicable as a smart multifunctional agents for cancer therapy.

UR - http://hdl.handle.net/10447/367202

M3 - Article

VL - 11

SP - 889-

JO - Polymers

JF - Polymers

SN - 2073-4360

ER -