TY - JOUR
T1 - COLON MICROBIAL COMPOSITION IS CORRELATED WITH THE SEVERITY OF COLITIS INDUCED BY 2,4,6-TRINITROBENZESULFONIC ACID IN MICE.
AU - Tomasello, Giovanni
AU - Barbato, Olimpia
AU - Menchetti, Laura
AU - Piro, Federica
AU - Proietti, Patrizia Casagrande
AU - Leonardi, Leonardo
AU - Tomasello, Giovanni
AU - Proietti, Patrizia Casagrande
AU - Proietti, Patrizia Casagrande
AU - Traina, Giovanna
AU - Brecchia, Gabriele
PY - 2016
Y1 - 2016
N2 - The objective of this study was to evaluate the changes in some bacterial species of colonic microbiota, the clinical signs and the intestinal changes in mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS) induced colitis. CD-1 male mice were randomly divided into three groups and inoculated intrarectally with saline ethanol or TNBS solutions. ethanol and TNBS treatments induced weight loss accompained by mild and severe inflammation of the colon mucosa, respectively. however, TNBS-treated mice displayed significant differences compared to the saline group in terms of disease activity index and histological scoring. Both ethanol and TNBS groups showed an increased prevalence of escherichia coli and Clostridium supp., a decrease in lactobacilli and Bifidobacteria counts, as well as changes in the relative proportions of bacteria in the colon. The results confirm the validity of TNBS treatment to study the mechanism involved in the pathogenesis and progression of inflammatory bowel diseases (IBD) in CD-1 mice. Gut microbiota may become a diagnostic biomarker with therapeutic potential for IBD in the future.
AB - The objective of this study was to evaluate the changes in some bacterial species of colonic microbiota, the clinical signs and the intestinal changes in mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS) induced colitis. CD-1 male mice were randomly divided into three groups and inoculated intrarectally with saline ethanol or TNBS solutions. ethanol and TNBS treatments induced weight loss accompained by mild and severe inflammation of the colon mucosa, respectively. however, TNBS-treated mice displayed significant differences compared to the saline group in terms of disease activity index and histological scoring. Both ethanol and TNBS groups showed an increased prevalence of escherichia coli and Clostridium supp., a decrease in lactobacilli and Bifidobacteria counts, as well as changes in the relative proportions of bacteria in the colon. The results confirm the validity of TNBS treatment to study the mechanism involved in the pathogenesis and progression of inflammatory bowel diseases (IBD) in CD-1 mice. Gut microbiota may become a diagnostic biomarker with therapeutic potential for IBD in the future.
UR - http://hdl.handle.net/10447/219723
M3 - Article
SN - 2279-7165
VL - 11
SP - 165
EP - 175
JO - EuroMediterranean Biomedical Journal
JF - EuroMediterranean Biomedical Journal
ER -