Clinical relevance of thymidylate syntetase expression in the signet ring cell histotype component of colorectal carcinoma

Rosario Sanguedolce, Daniela Cabibi, Federico Aragona, Anna Calascibetta, Aragona, Campione, Dardanoni, Calascibetta, Rosario Sanguedolce, Cabibi, Barresi, Rausa, Elisabetta Barresi, Luciano Rausa, Maria Campione

Risultato della ricerca: Article

8 Citazioni (Scopus)

Abstract

Thymidylate Synthase (TS) is the key enzyme for DNA synthesis pathways and is inhibited by 5-fluorouracil (5FU). The aim of this work was to study TS expression and the proliferation rate in the different histological types of colorectal carcinoma (CRC). 50 patients with CRC were included in this study and evaluated immunohistochemically using the monoclonal antibodies, TS106 and Ki67. 20 tumours were of the intestinal type, 15 cases were signet ring cell carcinoma (SRCCs) and 15 cases were "mixed-type", with at least two different histological components. Intestinal and mucinous histotypes were positive for TS and Ki67, while "signet ring cell" samples were negative or showed only weak and focal positivity for both the TS and Ki67 antibodies. Our results show that signet ring cells (that are also often present in intestinal and mucinous carcinomas), are in the post-mitotic phase of the cell cycle and show a low proliferation index and TS expression. As TS is the key enzyme for DNA synthesis pathways and is inhibited by 5-fluorouracil (5FU), we can hypothesise that TS expression levels in the different histotypes of CRC could affect the potential responsiveness of these tumours to fluoropyrimidine chemotherapy, with a low efficacy being expected in signet ring cell areas.
Lingua originaleEnglish
pagine (da-a)2845-2850
Numero di pagine6
RivistaEuropean Journal of Cancer
Volume40
Stato di pubblicazionePublished - 2004

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Thymidylate Synthase
Cellular Structures
Colorectal Neoplasms
Fluorouracil
Signet Ring Cell Carcinoma
Mucinous Adenocarcinoma
DNA
Enzymes
Neoplasms
Cell Cycle
Monoclonal Antibodies
Drug Therapy
Antibodies

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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Clinical relevance of thymidylate syntetase expression in the signet ring cell histotype component of colorectal carcinoma. / Sanguedolce, Rosario; Cabibi, Daniela; Aragona, Federico; Calascibetta, Anna; Aragona; Campione; Dardanoni; Calascibetta; Sanguedolce, Rosario; Cabibi; Barresi; Rausa; Barresi, Elisabetta; Rausa, Luciano; Campione, Maria.

In: European Journal of Cancer, Vol. 40, 2004, pag. 2845-2850.

Risultato della ricerca: Article

Sanguedolce, R, Cabibi, D, Aragona, F, Calascibetta, A, Aragona, Campione, Dardanoni, Calascibetta, Sanguedolce, R, Cabibi, Barresi, Rausa, Barresi, E, Rausa, L & Campione, M 2004, 'Clinical relevance of thymidylate syntetase expression in the signet ring cell histotype component of colorectal carcinoma', European Journal of Cancer, vol. 40, pagg. 2845-2850.
Sanguedolce, Rosario ; Cabibi, Daniela ; Aragona, Federico ; Calascibetta, Anna ; Aragona ; Campione ; Dardanoni ; Calascibetta ; Sanguedolce, Rosario ; Cabibi ; Barresi ; Rausa ; Barresi, Elisabetta ; Rausa, Luciano ; Campione, Maria. / Clinical relevance of thymidylate syntetase expression in the signet ring cell histotype component of colorectal carcinoma. In: European Journal of Cancer. 2004 ; Vol. 40. pagg. 2845-2850.
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abstract = "Thymidylate Synthase (TS) is the key enzyme for DNA synthesis pathways and is inhibited by 5-fluorouracil (5FU). The aim of this work was to study TS expression and the proliferation rate in the different histological types of colorectal carcinoma (CRC). 50 patients with CRC were included in this study and evaluated immunohistochemically using the monoclonal antibodies, TS106 and Ki67. 20 tumours were of the intestinal type, 15 cases were signet ring cell carcinoma (SRCCs) and 15 cases were {"}mixed-type{"}, with at least two different histological components. Intestinal and mucinous histotypes were positive for TS and Ki67, while {"}signet ring cell{"} samples were negative or showed only weak and focal positivity for both the TS and Ki67 antibodies. Our results show that signet ring cells (that are also often present in intestinal and mucinous carcinomas), are in the post-mitotic phase of the cell cycle and show a low proliferation index and TS expression. As TS is the key enzyme for DNA synthesis pathways and is inhibited by 5-fluorouracil (5FU), we can hypothesise that TS expression levels in the different histotypes of CRC could affect the potential responsiveness of these tumours to fluoropyrimidine chemotherapy, with a low efficacy being expected in signet ring cell areas.",
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T1 - Clinical relevance of thymidylate syntetase expression in the signet ring cell histotype component of colorectal carcinoma

AU - Sanguedolce, Rosario

AU - Cabibi, Daniela

AU - Aragona, Federico

AU - Calascibetta, Anna

AU - Aragona, null

AU - Campione, null

AU - Dardanoni, null

AU - Calascibetta, null

AU - Sanguedolce, Rosario

AU - Cabibi, null

AU - Barresi, null

AU - Rausa, null

AU - Barresi, Elisabetta

AU - Rausa, Luciano

AU - Campione, Maria

PY - 2004

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N2 - Thymidylate Synthase (TS) is the key enzyme for DNA synthesis pathways and is inhibited by 5-fluorouracil (5FU). The aim of this work was to study TS expression and the proliferation rate in the different histological types of colorectal carcinoma (CRC). 50 patients with CRC were included in this study and evaluated immunohistochemically using the monoclonal antibodies, TS106 and Ki67. 20 tumours were of the intestinal type, 15 cases were signet ring cell carcinoma (SRCCs) and 15 cases were "mixed-type", with at least two different histological components. Intestinal and mucinous histotypes were positive for TS and Ki67, while "signet ring cell" samples were negative or showed only weak and focal positivity for both the TS and Ki67 antibodies. Our results show that signet ring cells (that are also often present in intestinal and mucinous carcinomas), are in the post-mitotic phase of the cell cycle and show a low proliferation index and TS expression. As TS is the key enzyme for DNA synthesis pathways and is inhibited by 5-fluorouracil (5FU), we can hypothesise that TS expression levels in the different histotypes of CRC could affect the potential responsiveness of these tumours to fluoropyrimidine chemotherapy, with a low efficacy being expected in signet ring cell areas.

AB - Thymidylate Synthase (TS) is the key enzyme for DNA synthesis pathways and is inhibited by 5-fluorouracil (5FU). The aim of this work was to study TS expression and the proliferation rate in the different histological types of colorectal carcinoma (CRC). 50 patients with CRC were included in this study and evaluated immunohistochemically using the monoclonal antibodies, TS106 and Ki67. 20 tumours were of the intestinal type, 15 cases were signet ring cell carcinoma (SRCCs) and 15 cases were "mixed-type", with at least two different histological components. Intestinal and mucinous histotypes were positive for TS and Ki67, while "signet ring cell" samples were negative or showed only weak and focal positivity for both the TS and Ki67 antibodies. Our results show that signet ring cells (that are also often present in intestinal and mucinous carcinomas), are in the post-mitotic phase of the cell cycle and show a low proliferation index and TS expression. As TS is the key enzyme for DNA synthesis pathways and is inhibited by 5-fluorouracil (5FU), we can hypothesise that TS expression levels in the different histotypes of CRC could affect the potential responsiveness of these tumours to fluoropyrimidine chemotherapy, with a low efficacy being expected in signet ring cell areas.

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