TY - JOUR
T1 - Citrus limon-derived nanovesicles inhibit cancer cell proliferation and suppress CML xenograft growth by inducing TRAIL-mediated cell death
AU - Monteleone, Francesca
AU - Zito, Giovanni
AU - Lo Dico, Alessia
AU - Fontana, Simona
AU - De Leo, Giacomo
AU - Di Bella, Maria Antonietta
AU - Saieva, Laura
AU - Raimondo, Stefania
AU - Naselli, Flores
AU - Alessandro, Riccardo
AU - Manno, Mauro
AU - Zito, Giovanni
AU - Manno, Mauro
PY - 2015
Y1 - 2015
N2 - Nanosized vesicles are considered key players in cell to cell communication, thus influencing physiological and pathological processes, including cancer. Nanovesicles have also been found in edible-plants and have shown therapeutic activity in inflammatory bowel diseases; however information on their role in affecting cancer progression is missing.Our study identify for the first time a fraction of vesicles from lemon juice (Citrus limon L.), obtained as a result of different ultracentrifugation, with density ranging from 1,15 to 1,19 g/ml and specific proteomic profile. By using an in vitro approach, we show that isolated nanovesicles inhibit cancer cell proliferation in different tumor cell lines, by activating a TRAIL-mediated apoptotic cell death. Furthermore, we demonstrate that lemon nanovesicles suppress CML tumor growth in vivo by specifically reaching tumor site and by activating TRAIL-mediated apoptotic cell processes. Overall, this study suggests the possible use of plant-edible nanovesicles as a feasible approach in cancer treatment.
AB - Nanosized vesicles are considered key players in cell to cell communication, thus influencing physiological and pathological processes, including cancer. Nanovesicles have also been found in edible-plants and have shown therapeutic activity in inflammatory bowel diseases; however information on their role in affecting cancer progression is missing.Our study identify for the first time a fraction of vesicles from lemon juice (Citrus limon L.), obtained as a result of different ultracentrifugation, with density ranging from 1,15 to 1,19 g/ml and specific proteomic profile. By using an in vitro approach, we show that isolated nanovesicles inhibit cancer cell proliferation in different tumor cell lines, by activating a TRAIL-mediated apoptotic cell death. Furthermore, we demonstrate that lemon nanovesicles suppress CML tumor growth in vivo by specifically reaching tumor site and by activating TRAIL-mediated apoptotic cell processes. Overall, this study suggests the possible use of plant-edible nanovesicles as a feasible approach in cancer treatment.
KW - Citrus limon L.
KW - TRAIL-mediated cell death
KW - cancer
KW - exosome-like nanovesicles
KW - Citrus limon L.
KW - TRAIL-mediated cell death
KW - cancer
KW - exosome-like nanovesicles
UR - http://hdl.handle.net/10447/136552
M3 - Article
VL - 6
JO - Oncotarget
JF - Oncotarget
SN - 1949-2553
ER -