In humans, IL-17 is a proinflammatory cytokine that plays a key role in the clearance ofextracellular bacteria promoting cell infiltration and production of several cytokines andchemokines. Here, we report on three Ciona intestinalis IL-17 homologues (CiIL17-1, CiIL17-2,CiIL17-3). The gene organisation, phylogenetic tree and modelling supported the close relationshipwith the mammalian IL-17A and IL-17F suggesting that the C. intestinalis IL-17 genes share acommon ancestor in the chordate lineages. Real time PCR analysis showed a prompt expressioninduced by LPS inoculation suggesting that they are involved in the first phase of inflammatoryresponse. In situ hybridization assays disclosed that the genes transcription was upregulated in thepharynx, the main organ of the ascidian immune system, and expressed by hemocytes (granulocytesand univacuolar refractile granulocyte) inside the pharynx vessels.
|Numero di pagine||9|
|Rivista||Developmental and Comparative Immunology|
|Stato di pubblicazione||Published - 2015|
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