Immunohistochemical and in situ hybridization assays were performed to answer the question whetherthe endostyle, that is the initial gastro-intestinal trait of Ciona intestinalis pharynx, is involved in galectin(CiLgals-a and CiLgals-b) production during the pharynx inflammatory response to LPS inoculation.Specific anti-CiLgal-a and anti-CiLgals-b antibodies, and oligonucleotide probes, that mark inflammatoryhemocytes inside the pharynx vessels and vessel epithelium as shown by a previous paper, were assayedon endostyle histological sections. For the first time, we show that galectins are produced by endostylezones, and both CiLgals-a and eb genes are upregulated by LPS. CiLgals-a and CiLgals-b are constitutivelyexpressed in the endostyle zone 2 and 3, respectively, both genes are upregulated by LPS in the zone 2,and CiLgals-b in the zone 3 and 4. The antibody-reacting material contained in intracellular and extracellularlarge vesicles suggest an unexpected vesicle-dependent transporting mechanism of galectins notprovided with signal peptide. Differential expression and gene upregulation in not-treated and LPStreatedspecimens, support the role of endostyle galectins both in filter feeding and defense responses.
|Numero di pagine||6|
|Rivista||FISH AND SHELLFISH IMMUNOLOGY|
|Stato di pubblicazione||Published - 2015|
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