Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus.

Sisino, G.

Risultato della ricerca: Article

18 Citazioni (Scopus)

Abstract

In this paper the chromosomal localization and molecular cloning and characterization of three 5S rDNA clusters of 700 bp (base pairs), 900 bp, and 950 bp in the sea urchin Paracentrotus lividus are reported. Southern blot hybridization demonstrated the existence of three 5S rDNA repeats of differing length in the P. lividus genome. Fluorescence in situ hybridization analysis, performed in parallel on both haploid and diploid metaphases and interphase nuclei using different 5S rDNA units as probes, localized these 5S rDNA clusters in 3 different pairs of P. lividus chromosomes. This is the first complete gene mapping not only in a sea urchin but also in the phylum of echinoderms as a whole.
Lingua originaleEnglish
pagine (da-a)867-870
Numero di pagine4
RivistaGenome
Volume50
Stato di pubblicazionePublished - 2007

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Paracentrotus
Sea Urchins
Ribosomal DNA
Base Pairing
Chromosome Mapping
Haploidy
Interphase
Molecular Cloning
Metaphase
Southern Blotting
Diploidy
Fluorescence In Situ Hybridization
Chromosomes
Genome

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Genetics
  • Molecular Biology

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title = "Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus.",
abstract = "In this paper the chromosomal localization and molecular cloning and characterization of three 5S rDNA clusters of 700 bp (base pairs), 900 bp, and 950 bp in the sea urchin Paracentrotus lividus are reported. Southern blot hybridization demonstrated the existence of three 5S rDNA repeats of differing length in the P. lividus genome. Fluorescence in situ hybridization analysis, performed in parallel on both haploid and diploid metaphases and interphase nuclei using different 5S rDNA units as probes, localized these 5S rDNA clusters in 3 different pairs of P. lividus chromosomes. This is the first complete gene mapping not only in a sea urchin but also in the phylum of echinoderms as a whole.",
author = "{Sisino, G.} and Rainero Barbieri and Fabio Caradonna and Andrea Clemente and Daniele Bellavia",
year = "2007",
language = "English",
volume = "50",
pages = "867--870",
journal = "Genome",
issn = "0831-2796",
publisher = "National Research Council of Canada",

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TY - JOUR

T1 - Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus.

AU - Sisino, G.

AU - Barbieri, Rainero

AU - Caradonna, Fabio

AU - Clemente, Andrea

AU - Bellavia, Daniele

PY - 2007

Y1 - 2007

N2 - In this paper the chromosomal localization and molecular cloning and characterization of three 5S rDNA clusters of 700 bp (base pairs), 900 bp, and 950 bp in the sea urchin Paracentrotus lividus are reported. Southern blot hybridization demonstrated the existence of three 5S rDNA repeats of differing length in the P. lividus genome. Fluorescence in situ hybridization analysis, performed in parallel on both haploid and diploid metaphases and interphase nuclei using different 5S rDNA units as probes, localized these 5S rDNA clusters in 3 different pairs of P. lividus chromosomes. This is the first complete gene mapping not only in a sea urchin but also in the phylum of echinoderms as a whole.

AB - In this paper the chromosomal localization and molecular cloning and characterization of three 5S rDNA clusters of 700 bp (base pairs), 900 bp, and 950 bp in the sea urchin Paracentrotus lividus are reported. Southern blot hybridization demonstrated the existence of three 5S rDNA repeats of differing length in the P. lividus genome. Fluorescence in situ hybridization analysis, performed in parallel on both haploid and diploid metaphases and interphase nuclei using different 5S rDNA units as probes, localized these 5S rDNA clusters in 3 different pairs of P. lividus chromosomes. This is the first complete gene mapping not only in a sea urchin but also in the phylum of echinoderms as a whole.

UR - http://hdl.handle.net/10447/31681

M3 - Article

VL - 50

SP - 867

EP - 870

JO - Genome

JF - Genome

SN - 0831-2796

ER -