Chemical stiffening of constructs between polymeric microparticles based on a hyaluronic acid derivative and mesenchymal stem cells: rheological and in vitro viability studies

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Abstract

Our research group has recently developed microparticles of a hyaluronic acid derivative used for bottom-up growth of microparticles/human mesenchymal stem cells (hMSCs). In this work, we investigated a strategy to increase the stiffening of aggregated constructs between microparticles and hMSCs. In particular, we applied a Michael-type crosslinking procedure between microparticles to allow a chemically driven and cell-compatible stiffening of constructs. Two batches of microparticles were functionalized with thiol and maleimide groups, respectively, and were then mixed to allow chemical crosslinking. The adhesion of hMSCs was controlled through addition of the adhesive peptide cyclo(-Arg-Gly-Asp-D-Phe-Cys) (cyRGDC). Rheological measurements performed in this study showed that the chemical stiffening strategy allows the G′ modulus of bottom-up growing constructs to be increased, while viability tests suggest that the chemical procedure did not negatively affect cell viability compared with constructs obtained without chemical crosslinking. © 2018 Society of Chemical Industry.
Lingua originaleEnglish
pagine (da-a)394-399
Numero di pagine6
RivistaPolymer International
Volume68
Stato di pubblicazionePublished - 2019

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Hyaluronic acid
Hyaluronic Acid
Stem cells
Crosslinking
Derivatives
Sulfhydryl Compounds
Peptides
Adhesives
Adhesion
Cells

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

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title = "Chemical stiffening of constructs between polymeric microparticles based on a hyaluronic acid derivative and mesenchymal stem cells: rheological and in vitro viability studies",
abstract = "Our research group has recently developed microparticles of a hyaluronic acid derivative used for bottom-up growth of microparticles/human mesenchymal stem cells (hMSCs). In this work, we investigated a strategy to increase the stiffening of aggregated constructs between microparticles and hMSCs. In particular, we applied a Michael-type crosslinking procedure between microparticles to allow a chemically driven and cell-compatible stiffening of constructs. Two batches of microparticles were functionalized with thiol and maleimide groups, respectively, and were then mixed to allow chemical crosslinking. The adhesion of hMSCs was controlled through addition of the adhesive peptide cyclo(-Arg-Gly-Asp-D-Phe-Cys) (cyRGDC). Rheological measurements performed in this study showed that the chemical stiffening strategy allows the G′ modulus of bottom-up growing constructs to be increased, while viability tests suggest that the chemical procedure did not negatively affect cell viability compared with constructs obtained without chemical crosslinking. {\circledC} 2018 Society of Chemical Industry.",
keywords = "hyaluronic acid; mesenchymal stem cells, mechanical properties; microparticles; rheology; Polymers and Plastics; Organic Chemistry; Materials Chemistry2506 Metals and Alloys",
author = "Giovanna Pitarresi and Calogero Fiorica and Palumbo, {Fabio Salvatore} and Stefano Agnello and Gaetano Giammona and Gaetano Giammona",
year = "2019",
language = "English",
volume = "68",
pages = "394--399",
journal = "Polymer International",
issn = "0959-8103",
publisher = "John Wiley and Sons Ltd",

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TY - JOUR

T1 - Chemical stiffening of constructs between polymeric microparticles based on a hyaluronic acid derivative and mesenchymal stem cells: rheological and in vitro viability studies

AU - Pitarresi, Giovanna

AU - Fiorica, Calogero

AU - Palumbo, Fabio Salvatore

AU - Agnello, Stefano

AU - Giammona, Gaetano

AU - Giammona, Gaetano

PY - 2019

Y1 - 2019

N2 - Our research group has recently developed microparticles of a hyaluronic acid derivative used for bottom-up growth of microparticles/human mesenchymal stem cells (hMSCs). In this work, we investigated a strategy to increase the stiffening of aggregated constructs between microparticles and hMSCs. In particular, we applied a Michael-type crosslinking procedure between microparticles to allow a chemically driven and cell-compatible stiffening of constructs. Two batches of microparticles were functionalized with thiol and maleimide groups, respectively, and were then mixed to allow chemical crosslinking. The adhesion of hMSCs was controlled through addition of the adhesive peptide cyclo(-Arg-Gly-Asp-D-Phe-Cys) (cyRGDC). Rheological measurements performed in this study showed that the chemical stiffening strategy allows the G′ modulus of bottom-up growing constructs to be increased, while viability tests suggest that the chemical procedure did not negatively affect cell viability compared with constructs obtained without chemical crosslinking. © 2018 Society of Chemical Industry.

AB - Our research group has recently developed microparticles of a hyaluronic acid derivative used for bottom-up growth of microparticles/human mesenchymal stem cells (hMSCs). In this work, we investigated a strategy to increase the stiffening of aggregated constructs between microparticles and hMSCs. In particular, we applied a Michael-type crosslinking procedure between microparticles to allow a chemically driven and cell-compatible stiffening of constructs. Two batches of microparticles were functionalized with thiol and maleimide groups, respectively, and were then mixed to allow chemical crosslinking. The adhesion of hMSCs was controlled through addition of the adhesive peptide cyclo(-Arg-Gly-Asp-D-Phe-Cys) (cyRGDC). Rheological measurements performed in this study showed that the chemical stiffening strategy allows the G′ modulus of bottom-up growing constructs to be increased, while viability tests suggest that the chemical procedure did not negatively affect cell viability compared with constructs obtained without chemical crosslinking. © 2018 Society of Chemical Industry.

KW - hyaluronic acid; mesenchymal stem cells

KW - mechanical properties; microparticles; rheology; Polymers and Plastics; Organic Chemistry; Materials Chemistry2506 Metals and Alloys

UR - http://hdl.handle.net/10447/342940

UR - http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0126

M3 - Article

VL - 68

SP - 394

EP - 399

JO - Polymer International

JF - Polymer International

SN - 0959-8103

ER -