Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 394-399 |
| Numero di pagine | 6 |
| Rivista | Polymer International |
| Volume | 68 |
| Stato di pubblicazione | Published - 2019 |
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All Science Journal Classification (ASJC) codes
- Polymers and Plastics
- Organic Chemistry
- Materials Chemistry
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Chemical stiffening of constructs between polymeric microparticles based on a hyaluronic acid derivative and mesenchymal stem cells: rheological and in vitro viability studies. / Pitarresi, Giovanna; Fiorica, Calogero; Palumbo, Fabio Salvatore; Agnello, Stefano; Giammona, Gaetano; Giammona, Gaetano.
In: Polymer International, Vol. 68, 2019, pag. 394-399.Risultato della ricerca: Article
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TY - JOUR
T1 - Chemical stiffening of constructs between polymeric microparticles based on a hyaluronic acid derivative and mesenchymal stem cells: rheological and in vitro viability studies
AU - Pitarresi, Giovanna
AU - Fiorica, Calogero
AU - Palumbo, Fabio Salvatore
AU - Agnello, Stefano
AU - Giammona, Gaetano
AU - Giammona, Gaetano
PY - 2019
Y1 - 2019
N2 - Our research group has recently developed microparticles of a hyaluronic acid derivative used for bottom-up growth of microparticles/human mesenchymal stem cells (hMSCs). In this work, we investigated a strategy to increase the stiffening of aggregated constructs between microparticles and hMSCs. In particular, we applied a Michael-type crosslinking procedure between microparticles to allow a chemically driven and cell-compatible stiffening of constructs. Two batches of microparticles were functionalized with thiol and maleimide groups, respectively, and were then mixed to allow chemical crosslinking. The adhesion of hMSCs was controlled through addition of the adhesive peptide cyclo(-Arg-Gly-Asp-D-Phe-Cys) (cyRGDC). Rheological measurements performed in this study showed that the chemical stiffening strategy allows the G′ modulus of bottom-up growing constructs to be increased, while viability tests suggest that the chemical procedure did not negatively affect cell viability compared with constructs obtained without chemical crosslinking. © 2018 Society of Chemical Industry.
AB - Our research group has recently developed microparticles of a hyaluronic acid derivative used for bottom-up growth of microparticles/human mesenchymal stem cells (hMSCs). In this work, we investigated a strategy to increase the stiffening of aggregated constructs between microparticles and hMSCs. In particular, we applied a Michael-type crosslinking procedure between microparticles to allow a chemically driven and cell-compatible stiffening of constructs. Two batches of microparticles were functionalized with thiol and maleimide groups, respectively, and were then mixed to allow chemical crosslinking. The adhesion of hMSCs was controlled through addition of the adhesive peptide cyclo(-Arg-Gly-Asp-D-Phe-Cys) (cyRGDC). Rheological measurements performed in this study showed that the chemical stiffening strategy allows the G′ modulus of bottom-up growing constructs to be increased, while viability tests suggest that the chemical procedure did not negatively affect cell viability compared with constructs obtained without chemical crosslinking. © 2018 Society of Chemical Industry.
KW - hyaluronic acid; mesenchymal stem cells
KW - mechanical properties; microparticles; rheology; Polymers and Plastics; Organic Chemistry; Materials Chemistry2506 Metals and Alloys
UR - http://hdl.handle.net/10447/342940
UR - http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0126
M3 - Article
VL - 68
SP - 394
EP - 399
JO - Polymer International
JF - Polymer International
SN - 0959-8103
ER -