Characterization of transfected HT-29 cells expressing the oncogenic Ras isoform KrasG13D.

Risultato della ricerca: Paper

Abstract

Point mutations in codon 12 and 13 of K-ras are frequently found in DNA of colorectal cancer. It has been suggested that particular mutations at these sites may be associated with specific tumour phenotypes. To shed light on the molecular mechanisms on which depends this specificity we set up a system of HT-29 cells stably transfected with a cDNA coding for K-rasG13D under the control of an inducible promoter. Proliferation assay performed on one of the positives clones, showed a decreased growth rate in response to K-rasG13D expression and preliminary gene expression analysis showed an up-regulation of the cell-cycle inhibitor p21 WAF1.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2010

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HT29 Cells
Point Mutation
Codon
Colorectal Neoplasms
Cell Cycle
Protein Isoforms
Up-Regulation
Complementary DNA
Clone Cells
Phenotype
Gene Expression
Mutation
DNA
Growth
Neoplasms

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title = "Characterization of transfected HT-29 cells expressing the oncogenic Ras isoform KrasG13D.",
abstract = "Point mutations in codon 12 and 13 of K-ras are frequently found in DNA of colorectal cancer. It has been suggested that particular mutations at these sites may be associated with specific tumour phenotypes. To shed light on the molecular mechanisms on which depends this specificity we set up a system of HT-29 cells stably transfected with a cDNA coding for K-rasG13D under the control of an inducible promoter. Proliferation assay performed on one of the positives clones, showed a decreased growth rate in response to K-rasG13D expression and preliminary gene expression analysis showed an up-regulation of the cell-cycle inhibitor p21 WAF1.",
keywords = "K-ras; HT-29; proliferation",
author = "{La Farina}, Mario and Ida Albanese and Paolo Colomba and Vincenzo Eterno and Maurizio Bellavia and Fabrizio Miranda",
year = "2010",
language = "English",

}

TY - CONF

T1 - Characterization of transfected HT-29 cells expressing the oncogenic Ras isoform KrasG13D.

AU - La Farina, Mario

AU - Albanese, Ida

AU - Colomba, Paolo

AU - Eterno, Vincenzo

AU - Bellavia, Maurizio

AU - Miranda, Fabrizio

PY - 2010

Y1 - 2010

N2 - Point mutations in codon 12 and 13 of K-ras are frequently found in DNA of colorectal cancer. It has been suggested that particular mutations at these sites may be associated with specific tumour phenotypes. To shed light on the molecular mechanisms on which depends this specificity we set up a system of HT-29 cells stably transfected with a cDNA coding for K-rasG13D under the control of an inducible promoter. Proliferation assay performed on one of the positives clones, showed a decreased growth rate in response to K-rasG13D expression and preliminary gene expression analysis showed an up-regulation of the cell-cycle inhibitor p21 WAF1.

AB - Point mutations in codon 12 and 13 of K-ras are frequently found in DNA of colorectal cancer. It has been suggested that particular mutations at these sites may be associated with specific tumour phenotypes. To shed light on the molecular mechanisms on which depends this specificity we set up a system of HT-29 cells stably transfected with a cDNA coding for K-rasG13D under the control of an inducible promoter. Proliferation assay performed on one of the positives clones, showed a decreased growth rate in response to K-rasG13D expression and preliminary gene expression analysis showed an up-regulation of the cell-cycle inhibitor p21 WAF1.

KW - K-ras; HT-29; proliferation

UR - http://hdl.handle.net/10447/44554

UR - http://www.unipa.it/dipbio/congresso2009/congresso2009.html

M3 - Paper

ER -