Characterization of the first extended-spectrum b-lactamase–producing nontyphoidal Salmonella strains Isolated in Tehran, Iran

Giovanni Giammanco, Caterina Mammina, Parviz Owlia, Ali Naghoni, Reza Ranjbar, Aurora Aleo, Maria Rosa Anna Plano

Risultato della ricerca: Article

33 Citazioni (Scopus)

Abstract

The infections caused by Salmonella remain a significant public health problem throughout the world. b-Lactams and fluoroquinolones are generally used to treat invasive Salmonella infections, but emergence and spread of antibiotic-resistant strains are being increasingly notified in many countries. In particular, detection of extended spectrum b-lactamases (ESBLs) in Salmonella spp. is a newly emerging threat worldwide. This study was carriedout to characterize b-lactamase–producing Salmonella strains identified in Tehran, Iran. Over the 2-year period from 2007 to 2008, 6 of 136 Salmonella isolates recovered from pediatrics patients, including three Salmonella enterica serotypes Enteritidis (S. Enteritidis) and three S. Infantis, showed an ESBL-positive phenotype. Polymerasechain reaction and sequencing were used to identify the genetic determinants responsible for ESBL phenotypes. The Salmonella isolates were also compared by pulsed-field gel electrophoresis. All ESBL-producing strains, but one, carried the blaCTX-M-15 gene. Moreover, three of four strains that proved to be positive for ablaTEM gene were producing a TEM-1 b-lactamase. Two strains of S. Infantis tested positive for a previously unidentified CTX-M and TEM ESBL, respectively. All ESBL-producing strains carried the insertion sequence ISEcp1 gene. Except for one strain of serotype Infantis, all strains were able to transfer the ESBL determinants byconjugation. Distinct, but closely related, pulsed-field gel electrophoresis patterns were observed among the strains belonging to both serotypes. This study reports for the first time the emergence and characterization of ESBL-producing S. Enteritidis and Infantis strains in Iran.
Lingua originaleEnglish
pagine (da-a)91-95
Numero di pagine5
RivistaFoodborne Pathogens and Disease
Volume7
Stato di pubblicazionePublished - 2010

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Iran
Salmonella
Salmonella Infections
Pulsed Field Gel Electrophoresis
Genes
Phenotype
serotypes
Lactams
Salmonella enterica
Insertional Mutagenesis
Fluoroquinolones
pulsed-field gel electrophoresis
Public Health
Pediatrics
Anti-Bacterial Agents
Serogroup
Salmonella Infantis
lactams
phenotype
fluoroquinolones

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Food Science
  • Animal Science and Zoology

Cita questo

Characterization of the first extended-spectrum b-lactamase–producing nontyphoidal Salmonella strains Isolated in Tehran, Iran. / Giammanco, Giovanni; Mammina, Caterina; Owlia, Parviz; Naghoni, Ali; Ranjbar, Reza; Aleo, Aurora; Plano, Maria Rosa Anna.

In: Foodborne Pathogens and Disease, Vol. 7, 2010, pag. 91-95.

Risultato della ricerca: Article

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title = "Characterization of the first extended-spectrum b-lactamase–producing nontyphoidal Salmonella strains Isolated in Tehran, Iran",
abstract = "The infections caused by Salmonella remain a significant public health problem throughout the world. b-Lactams and fluoroquinolones are generally used to treat invasive Salmonella infections, but emergence and spread of antibiotic-resistant strains are being increasingly notified in many countries. In particular, detection of extended spectrum b-lactamases (ESBLs) in Salmonella spp. is a newly emerging threat worldwide. This study was carriedout to characterize b-lactamase–producing Salmonella strains identified in Tehran, Iran. Over the 2-year period from 2007 to 2008, 6 of 136 Salmonella isolates recovered from pediatrics patients, including three Salmonella enterica serotypes Enteritidis (S. Enteritidis) and three S. Infantis, showed an ESBL-positive phenotype. Polymerasechain reaction and sequencing were used to identify the genetic determinants responsible for ESBL phenotypes. The Salmonella isolates were also compared by pulsed-field gel electrophoresis. All ESBL-producing strains, but one, carried the blaCTX-M-15 gene. Moreover, three of four strains that proved to be positive for ablaTEM gene were producing a TEM-1 b-lactamase. Two strains of S. Infantis tested positive for a previously unidentified CTX-M and TEM ESBL, respectively. All ESBL-producing strains carried the insertion sequence ISEcp1 gene. Except for one strain of serotype Infantis, all strains were able to transfer the ESBL determinants byconjugation. Distinct, but closely related, pulsed-field gel electrophoresis patterns were observed among the strains belonging to both serotypes. This study reports for the first time the emergence and characterization of ESBL-producing S. Enteritidis and Infantis strains in Iran.",
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T1 - Characterization of the first extended-spectrum b-lactamase–producing nontyphoidal Salmonella strains Isolated in Tehran, Iran

AU - Giammanco, Giovanni

AU - Mammina, Caterina

AU - Owlia, Parviz

AU - Naghoni, Ali

AU - Ranjbar, Reza

AU - Aleo, Aurora

AU - Plano, Maria Rosa Anna

PY - 2010

Y1 - 2010

N2 - The infections caused by Salmonella remain a significant public health problem throughout the world. b-Lactams and fluoroquinolones are generally used to treat invasive Salmonella infections, but emergence and spread of antibiotic-resistant strains are being increasingly notified in many countries. In particular, detection of extended spectrum b-lactamases (ESBLs) in Salmonella spp. is a newly emerging threat worldwide. This study was carriedout to characterize b-lactamase–producing Salmonella strains identified in Tehran, Iran. Over the 2-year period from 2007 to 2008, 6 of 136 Salmonella isolates recovered from pediatrics patients, including three Salmonella enterica serotypes Enteritidis (S. Enteritidis) and three S. Infantis, showed an ESBL-positive phenotype. Polymerasechain reaction and sequencing were used to identify the genetic determinants responsible for ESBL phenotypes. The Salmonella isolates were also compared by pulsed-field gel electrophoresis. All ESBL-producing strains, but one, carried the blaCTX-M-15 gene. Moreover, three of four strains that proved to be positive for ablaTEM gene were producing a TEM-1 b-lactamase. Two strains of S. Infantis tested positive for a previously unidentified CTX-M and TEM ESBL, respectively. All ESBL-producing strains carried the insertion sequence ISEcp1 gene. Except for one strain of serotype Infantis, all strains were able to transfer the ESBL determinants byconjugation. Distinct, but closely related, pulsed-field gel electrophoresis patterns were observed among the strains belonging to both serotypes. This study reports for the first time the emergence and characterization of ESBL-producing S. Enteritidis and Infantis strains in Iran.

AB - The infections caused by Salmonella remain a significant public health problem throughout the world. b-Lactams and fluoroquinolones are generally used to treat invasive Salmonella infections, but emergence and spread of antibiotic-resistant strains are being increasingly notified in many countries. In particular, detection of extended spectrum b-lactamases (ESBLs) in Salmonella spp. is a newly emerging threat worldwide. This study was carriedout to characterize b-lactamase–producing Salmonella strains identified in Tehran, Iran. Over the 2-year period from 2007 to 2008, 6 of 136 Salmonella isolates recovered from pediatrics patients, including three Salmonella enterica serotypes Enteritidis (S. Enteritidis) and three S. Infantis, showed an ESBL-positive phenotype. Polymerasechain reaction and sequencing were used to identify the genetic determinants responsible for ESBL phenotypes. The Salmonella isolates were also compared by pulsed-field gel electrophoresis. All ESBL-producing strains, but one, carried the blaCTX-M-15 gene. Moreover, three of four strains that proved to be positive for ablaTEM gene were producing a TEM-1 b-lactamase. Two strains of S. Infantis tested positive for a previously unidentified CTX-M and TEM ESBL, respectively. All ESBL-producing strains carried the insertion sequence ISEcp1 gene. Except for one strain of serotype Infantis, all strains were able to transfer the ESBL determinants byconjugation. Distinct, but closely related, pulsed-field gel electrophoresis patterns were observed among the strains belonging to both serotypes. This study reports for the first time the emergence and characterization of ESBL-producing S. Enteritidis and Infantis strains in Iran.

KW - CTX-M-15

KW - ESBL

KW - Iran

KW - S. Enteritidis

KW - Salmonella

KW - TEM-1

KW - extended-spectrum b-lactamases

UR - http://hdl.handle.net/10447/42870

M3 - Article

VL - 7

SP - 91

EP - 95

JO - Foodborne Pathogens and Disease

JF - Foodborne Pathogens and Disease

SN - 1535-3141

ER -