Characterization of Leaf Essential Oil Composition ofHomozygous and Heterozygous Citrus clementina Hort.Extan. and its Ancestors

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Clementine is a natural tangor, resulting from an interspecific cross between mandarin andsweet orange. Gametic embryogenesis, allowing the single-step development of complete homozygous linefrom the heterozygous parents, increases the efficiency of perennial crop breeding programs. Tri-haploidshave been regenerated through pollen embryogenesis (specifically, by anther culture) of Citrus clementinaHort. ex Tan., cv. Nules. Two of them (HOMO1 and HOMO2) have been acclimatized and grafted in vivo in2000. Research regarding the chemical characteristics of plant regenerated by pollen embryogenesis orgynogenesis are useful to study the “gametoclonal variation”, as well as for further application of haploidytechnology in Citrus breeding. For these reasons and with the aim to characterize these homozygous clementinegenotypes, in this study leaf essential oils were extracted by Clevenger apparatus and their chemical compositionwas investigated by capillary GC-MS, comparing them with oils obtained from the heterozygous plant andfrom its ancestors (clementine is a hybrid between sweet orange and mandarin). The obtained oil compositions,submitted to a statistical analysis, are partitioned into groups using principal components analysis (PCA) and,compared to those of both parents, distinguished in three major chemotypes sabinene/linalool, γ-terpinene andmethyl N-methylanthranilate.
Lingua originaleEnglish
pagine (da-a)92-101
Numero di pagine10
RivistaJournal of Essential Oil-Bearing Plants
Stato di pubblicazionePublished - 2013


All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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