Abstract

Recently it has been hypothesized that a reactivation of the epithelial-mesenchymal trophic unit (EMTU) of the respiratory mucosa, caused by extensive epithelial damage, may be responsible for the pathogenesis of asthma. However, most research on this subject so far has been limited by the unavailability of in vitro models correctly mimicking the three-dimensional architecture of the properly differentiated human bronchial mucosa. For this reason, we have developed a novel 3D outgrowth model, obtained from bronchial biopsies and composed of both human bronchial epithelial cells (goblet and columnar cells) and fibroblasts with their basement membrane. Here we present the preliminary data obtained by both SEM and TEM on the characterization of these bronchial outgrowths showing that cells and matrices within the 3D structures are well differentiated, presenting all the normal features of human bronchial mucosa such as microvilli, cilia, mucus, tight junctions, collagens etc. We also show the effects of medium-term treatment with cigarette smoke extracts in terms of cell survival and remodeling.
Lingua originaleEnglish
Numero di pagine1
Stato di pubblicazionePublished - 2011

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Mucous Membrane
Respiratory Mucosa
Goblet Cells
Cilia
Tight Junctions
Mucus
Microvilli
Basement Membrane
Smoke
Tobacco Products
Cell Survival
Collagen
Asthma
Fibroblasts
Epithelial Cells
Biopsy
Research
In Vitro Techniques

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title = "Characterization of a novel three-dimensional outgrowth model of human bronchial mucosa",
abstract = "Recently it has been hypothesized that a reactivation of the epithelial-mesenchymal trophic unit (EMTU) of the respiratory mucosa, caused by extensive epithelial damage, may be responsible for the pathogenesis of asthma. However, most research on this subject so far has been limited by the unavailability of in vitro models correctly mimicking the three-dimensional architecture of the properly differentiated human bronchial mucosa. For this reason, we have developed a novel 3D outgrowth model, obtained from bronchial biopsies and composed of both human bronchial epithelial cells (goblet and columnar cells) and fibroblasts with their basement membrane. Here we present the preliminary data obtained by both SEM and TEM on the characterization of these bronchial outgrowths showing that cells and matrices within the 3D structures are well differentiated, presenting all the normal features of human bronchial mucosa such as microvilli, cilia, mucus, tight junctions, collagens etc. We also show the effects of medium-term treatment with cigarette smoke extracts in terms of cell survival and remodeling.",
keywords = "3d models, EMTU, human mucosae, scaffolds, tissue engineering",
author = "Fabio Bucchieri and {Marino Gammazza}, Antonella and Fucarino, {Alberto Giuseppe} and Francesco Cappello and Alessandro Pitruzzella and Giovanni Zummo and Vito Marciano'",
year = "2011",
language = "English",

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TY - CONF

T1 - Characterization of a novel three-dimensional outgrowth model of human bronchial mucosa

AU - Bucchieri, Fabio

AU - Marino Gammazza, Antonella

AU - Fucarino, Alberto Giuseppe

AU - Cappello, Francesco

AU - Pitruzzella, Alessandro

AU - Zummo, Giovanni

AU - Marciano', Vito

PY - 2011

Y1 - 2011

N2 - Recently it has been hypothesized that a reactivation of the epithelial-mesenchymal trophic unit (EMTU) of the respiratory mucosa, caused by extensive epithelial damage, may be responsible for the pathogenesis of asthma. However, most research on this subject so far has been limited by the unavailability of in vitro models correctly mimicking the three-dimensional architecture of the properly differentiated human bronchial mucosa. For this reason, we have developed a novel 3D outgrowth model, obtained from bronchial biopsies and composed of both human bronchial epithelial cells (goblet and columnar cells) and fibroblasts with their basement membrane. Here we present the preliminary data obtained by both SEM and TEM on the characterization of these bronchial outgrowths showing that cells and matrices within the 3D structures are well differentiated, presenting all the normal features of human bronchial mucosa such as microvilli, cilia, mucus, tight junctions, collagens etc. We also show the effects of medium-term treatment with cigarette smoke extracts in terms of cell survival and remodeling.

AB - Recently it has been hypothesized that a reactivation of the epithelial-mesenchymal trophic unit (EMTU) of the respiratory mucosa, caused by extensive epithelial damage, may be responsible for the pathogenesis of asthma. However, most research on this subject so far has been limited by the unavailability of in vitro models correctly mimicking the three-dimensional architecture of the properly differentiated human bronchial mucosa. For this reason, we have developed a novel 3D outgrowth model, obtained from bronchial biopsies and composed of both human bronchial epithelial cells (goblet and columnar cells) and fibroblasts with their basement membrane. Here we present the preliminary data obtained by both SEM and TEM on the characterization of these bronchial outgrowths showing that cells and matrices within the 3D structures are well differentiated, presenting all the normal features of human bronchial mucosa such as microvilli, cilia, mucus, tight junctions, collagens etc. We also show the effects of medium-term treatment with cigarette smoke extracts in terms of cell survival and remodeling.

KW - 3d models

KW - EMTU

KW - human mucosae

KW - scaffolds

KW - tissue engineering

UR - http://hdl.handle.net/10447/104379

M3 - Other

ER -