CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA.

Francesco Dieli, Giacomo De Leo, Alessia Lo Dico, Laura Saieva, Alice Conigliaro, Riccardo Alessandro, Simona Buccheri, Alice Conigliaro, Carmine Mancone, Mauro Manno, Samuele Raccosta, Viviana Costa, Riccardo Alessandro, Marco Tripodi, Simona Buccheri

Risultato della ricerca: Article

143 Citazioni (Scopus)

Abstract

BACKGROUND:CD90+ liver cancer cells have been described as cancer stem-cell-like (CSC), displaying aggressive and metastatic phenotype. Using two different in vitro models, already described as CD90+ liver cancer stem cells, our aim was to study their interaction with endothelial cells mediated by the release of exosomes.METHODS:Exosomes were isolated and characterized from both liver CD90+ cells and hepatoma cell lines. Endothelial cells were treated with exosomes, as well as transfected with a plasmid containing the full length sequence of the long non-coding RNA (lncRNA) H19. Molecular and functional analyses were done to characterize the endothelial phenotype after treatments.RESULTS:Exosomes released by CD90+ cancer cells, but not by parental hepatoma cells, modulated endothelial cells, promoting angiogenic phenotype and cell-to-cell adhesion. LncRNA profiling revealed that CD90+ cells were enriched in lncRNA H19, and released this through exosomes. Experiments of gain and loss of function of H19 showed that this LncRNA plays an important role in the exosome-mediated phenotype of endothelial cells.CONCLUSIONS:Our data indicate a new exosome-mediated mechanism by which CSC-like CD90+ cells could influence their tumor microenvironment by promoting angiogenesis. Moreover, we suggest the lncRNA H19 as a putative therapeutic target in hepatocellular carcinoma.
Lingua originaleEnglish
pagine (da-a)155-165
Numero di pagine11
RivistaMolecular Cancer
Volume14
Stato di pubblicazionePublished - 2015

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Exosomes
Liver Neoplasms
Endothelial Cells
Phenotype
Neoplastic Stem Cells
Hepatocellular Carcinoma
Tumor Microenvironment
H19 long non-coding RNA
Cell Adhesion
Plasmids
Cell Line
Liver

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Oncology
  • Cancer Research

Cita questo

CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA. / Dieli, Francesco; De Leo, Giacomo; Lo Dico, Alessia; Saieva, Laura; Conigliaro, Alice; Alessandro, Riccardo; Buccheri, Simona; Conigliaro, Alice; Mancone, Carmine; Manno, Mauro; Raccosta, Samuele; Costa, Viviana; Alessandro, Riccardo; Tripodi, Marco; Buccheri, Simona.

In: Molecular Cancer, Vol. 14, 2015, pag. 155-165.

Risultato della ricerca: Article

Dieli, F, De Leo, G, Lo Dico, A, Saieva, L, Conigliaro, A, Alessandro, R, Buccheri, S, Conigliaro, A, Mancone, C, Manno, M, Raccosta, S, Costa, V, Alessandro, R, Tripodi, M & Buccheri, S 2015, 'CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA.', Molecular Cancer, vol. 14, pagg. 155-165.
Dieli, Francesco ; De Leo, Giacomo ; Lo Dico, Alessia ; Saieva, Laura ; Conigliaro, Alice ; Alessandro, Riccardo ; Buccheri, Simona ; Conigliaro, Alice ; Mancone, Carmine ; Manno, Mauro ; Raccosta, Samuele ; Costa, Viviana ; Alessandro, Riccardo ; Tripodi, Marco ; Buccheri, Simona. / CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA. In: Molecular Cancer. 2015 ; Vol. 14. pagg. 155-165.
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title = "CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA.",
abstract = "BACKGROUND:CD90+ liver cancer cells have been described as cancer stem-cell-like (CSC), displaying aggressive and metastatic phenotype. Using two different in vitro models, already described as CD90+ liver cancer stem cells, our aim was to study their interaction with endothelial cells mediated by the release of exosomes.METHODS:Exosomes were isolated and characterized from both liver CD90+ cells and hepatoma cell lines. Endothelial cells were treated with exosomes, as well as transfected with a plasmid containing the full length sequence of the long non-coding RNA (lncRNA) H19. Molecular and functional analyses were done to characterize the endothelial phenotype after treatments.RESULTS:Exosomes released by CD90+ cancer cells, but not by parental hepatoma cells, modulated endothelial cells, promoting angiogenic phenotype and cell-to-cell adhesion. LncRNA profiling revealed that CD90+ cells were enriched in lncRNA H19, and released this through exosomes. Experiments of gain and loss of function of H19 showed that this LncRNA plays an important role in the exosome-mediated phenotype of endothelial cells.CONCLUSIONS:Our data indicate a new exosome-mediated mechanism by which CSC-like CD90+ cells could influence their tumor microenvironment by promoting angiogenesis. Moreover, we suggest the lncRNA H19 as a putative therapeutic target in hepatocellular carcinoma.",
author = "Francesco Dieli and {De Leo}, Giacomo and {Lo Dico}, Alessia and Laura Saieva and Alice Conigliaro and Riccardo Alessandro and Simona Buccheri and Alice Conigliaro and Carmine Mancone and Mauro Manno and Samuele Raccosta and Viviana Costa and Riccardo Alessandro and Marco Tripodi and Simona Buccheri",
year = "2015",
language = "English",
volume = "14",
pages = "155--165",
journal = "Molecular Cancer",
issn = "1476-4598",
publisher = "BioMed Central",

}

TY - JOUR

T1 - CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA.

AU - Dieli, Francesco

AU - De Leo, Giacomo

AU - Lo Dico, Alessia

AU - Saieva, Laura

AU - Conigliaro, Alice

AU - Alessandro, Riccardo

AU - Buccheri, Simona

AU - Conigliaro, Alice

AU - Mancone, Carmine

AU - Manno, Mauro

AU - Raccosta, Samuele

AU - Costa, Viviana

AU - Alessandro, Riccardo

AU - Tripodi, Marco

AU - Buccheri, Simona

PY - 2015

Y1 - 2015

N2 - BACKGROUND:CD90+ liver cancer cells have been described as cancer stem-cell-like (CSC), displaying aggressive and metastatic phenotype. Using two different in vitro models, already described as CD90+ liver cancer stem cells, our aim was to study their interaction with endothelial cells mediated by the release of exosomes.METHODS:Exosomes were isolated and characterized from both liver CD90+ cells and hepatoma cell lines. Endothelial cells were treated with exosomes, as well as transfected with a plasmid containing the full length sequence of the long non-coding RNA (lncRNA) H19. Molecular and functional analyses were done to characterize the endothelial phenotype after treatments.RESULTS:Exosomes released by CD90+ cancer cells, but not by parental hepatoma cells, modulated endothelial cells, promoting angiogenic phenotype and cell-to-cell adhesion. LncRNA profiling revealed that CD90+ cells were enriched in lncRNA H19, and released this through exosomes. Experiments of gain and loss of function of H19 showed that this LncRNA plays an important role in the exosome-mediated phenotype of endothelial cells.CONCLUSIONS:Our data indicate a new exosome-mediated mechanism by which CSC-like CD90+ cells could influence their tumor microenvironment by promoting angiogenesis. Moreover, we suggest the lncRNA H19 as a putative therapeutic target in hepatocellular carcinoma.

AB - BACKGROUND:CD90+ liver cancer cells have been described as cancer stem-cell-like (CSC), displaying aggressive and metastatic phenotype. Using two different in vitro models, already described as CD90+ liver cancer stem cells, our aim was to study their interaction with endothelial cells mediated by the release of exosomes.METHODS:Exosomes were isolated and characterized from both liver CD90+ cells and hepatoma cell lines. Endothelial cells were treated with exosomes, as well as transfected with a plasmid containing the full length sequence of the long non-coding RNA (lncRNA) H19. Molecular and functional analyses were done to characterize the endothelial phenotype after treatments.RESULTS:Exosomes released by CD90+ cancer cells, but not by parental hepatoma cells, modulated endothelial cells, promoting angiogenic phenotype and cell-to-cell adhesion. LncRNA profiling revealed that CD90+ cells were enriched in lncRNA H19, and released this through exosomes. Experiments of gain and loss of function of H19 showed that this LncRNA plays an important role in the exosome-mediated phenotype of endothelial cells.CONCLUSIONS:Our data indicate a new exosome-mediated mechanism by which CSC-like CD90+ cells could influence their tumor microenvironment by promoting angiogenesis. Moreover, we suggest the lncRNA H19 as a putative therapeutic target in hepatocellular carcinoma.

UR - http://hdl.handle.net/10447/189989

M3 - Article

VL - 14

SP - 155

EP - 165

JO - Molecular Cancer

JF - Molecular Cancer

SN - 1476-4598

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